<i>In vivo</i> characterization of transplanted human embryonic stem cell‐derived pancreatic endocrine islet cells

Eshpeter, Alana; Jiang, Jianming; Au, Melinda; Rajotte, Ray V.; Lu, Kuang‐Hui; Lebkowski, Jane; Majumdar, Anish Sen; Korbutt, Gregory S.

doi:10.1111/j.1365-2184.2008.00564.x

Cited by 39 publications

(29 citation statements)

References 37 publications

(79 reference statements)

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“…2a). [20][21][22][23][24][25][26][27] However, ESCs differentiated into b-cell precursors-characterized by transcription factors that 27 (b) Pancreatic precursors derived from hESCs differentiate into functional glucose responsive islet-like structures that revert hyperglycemia when implanted in vivo and allowed to mature, reproduced with permission from Nature Publishing group. 29 (c) Suspension culture of MIN6 cells creates islet-like structures that reverse hyperglycemia in vivo, while single cells do not, reproduced with permission from Elsevier.…”

Section: Stem Cellsmentioning

confidence: 99%

Tissue Engineering Approaches to Cell-Based Type 1 Diabetes Therapy

Amer

Mahoney

Bryant

2014

Tissue Engineering Part B: Reviews

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Type 1 diabetes mellitus is an autoimmune disease resulting from the destruction of insulin-producing pancreatic b-cells. Cell-based therapies, involving the transplantation of functional b-cells into diabetic patients, have been explored as a potential long-term treatment for this condition; however, success is limited. A tissue engineering approach of culturing insulin-producing cells with extracellular matrix (ECM) molecules in threedimensional (3D) constructs has the potential to enhance the efficacy of cell-based therapies for diabetes. When cultured in 3D environments, insulin-producing cells are often more viable and secrete more insulin than those in two dimensions. The addition of ECM molecules to the culture environments, depending on the specific type of molecule, can further enhance the viability and insulin secretion. This review addresses the different cell sources that can be utilized as b-cell replacements, the essential ECM molecules for the survival of these cells, and the 3D culture techniques that have been used to benefit cell function.

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Section: Stem Cellsmentioning

confidence: 99%

Tissue Engineering Approaches to Cell-Based Type 1 Diabetes Therapy

Amer

Mahoney

Bryant

2014

Tissue Engineering Part B: Reviews

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“…Furthermore, a critical aspect of these culture conditions was the removal of serum components present in hESC medium that could induce the proliferative and renewal capacity of stem cells (McLean et al 2007). The approach of using embryonic signals to instruct hESCs to definitive endoderm and subsequently to b cells was validated by several other groups using modifications of this procedure (Jiang et al 2007;Eshpeter et al 2008;Mao et al 2009). …”

Section: Lessons Learned From Embryonic Pancreas Development: Mimickimentioning

confidence: 99%

Generating Cells from Stem Cells--The Story So Far

Hebrok

2012

Cold Spring Harbor Perspectives in Medicine

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Despite intensive research, a treatment for diabetic patients that completely restores normoglycemia for an indefinite period of time remains elusive. Although islet transplantation temporarily confers normoglycemia to patients, the lack of a renewable source of insulinproducing b cells hampers the use of this treatment option. Although significant hurdles remain, recent advances in stem cell biology indicate that generation of fully matured b cells from uncommitted progenitor cells, including human embryonic stem cells and induced pluripotent stem cells derived from somatic cell populations, is becoming an achievable goal.

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“…Nevertheless, the finding that the insulin-producing cells differentiated in vitro were not homogeneous suggests the possibility of obtaining cells that would be useful for cell therapy by purification based on differences of their properties (Basford et al, 2011). Moreover, long-term survival and functioning in vivo were reported when pancreatic islet-like structures were prepared from insulin-producing cells that had been differentiated from human ES cells and transplanted into animals with diabetes (Eshpeter et al, 2008). The ability of ES cells and iPS cells to differentiate into insulin-producing cells has been verified by many reports.…”

Section: Kunisada Et Al Recently Developed a Methods Of Inducing Hummentioning

confidence: 99%