In VivoBiocompatibility of ZIF-8 for Slow Release via Intranasal Administration

Abstract

Zeolitic Imidazolate Framework-8 (ZIF-8) is becoming popular in research for its potential in antigen protection and for providing a thermally stable, slow-release platform. While papers applying these materials for immunological applications are aplenty in literature, studies that explore the biosafety of ZIF-8 in mammals - especially when administered intranasally - are not well represented. We checked the body clearance of uncoated and ZIF-coated liposomes and observed that the release slowed as ZIF-8 is easily degraded by mucosal fluid in the nasal cavity. We delivered varying doses of ZIF-8, checked their short- and long-term effects on diagnostic proteins found in blood serum, and found no noticeable differences from the saline control group. We also studied their lung diffusing capacity and tissue morphology; neither showed significant changes in morphology or function.

Biosurfactant production by Bacillus amyloliquefaciens, characterization and its potential applications

“…Their findings indicated 90% cell viability, suggesting minimal toxicity. Kumari et al (2023) assessed the cytotoxicity of the lipopeptide biosurfactant on HEA 293 cells using MTT assay, revealing negligible cytotoxic effects. The amphiphilic nature of lipopeptides allows them to interact with cell membranes without disrupting their integrity significantly.…”

A scalable synthesis of adjuvanting antigen depots based on metal–organic frameworks

“…The emergence of biomimetic mineralization of delicate biomaterials in a metal-organic framework (MOF) has allowed for the development of thermally and enzymatically protected biological material from numerous stressors. [24][25][26][27][28][29][30] Recent work has shown that the biofriendly zeolitic imidazolate framework-8 (ZIF-8)-encapsulated vaccines promote a more robust immune response against viral nanoparticles or whole-cell bacteria when injected subcutaneously. 24,25,[31][32][33] These results are striking, but in all cases, the vaccines used have been "self-adjuvanting" as the RNA or DNA within a virus or the lipopolysaccharides on the inactivated bacteria can induce their own strong immune response.…”

Supercharged precision killers: Genetically engineered biomimetic drugs of screened metalloantibiotics against Acinetobacter baumanni

“…To ensure the stability and efficient transport of Zn(Bq) 2 , we used zeolitic imidazolate framework-8 (ZIF-8) as a drug carrier platform. ZIF-8 was chosen because of its exceptional characteristics such as ultrahigh porosity, large specific surface area, pH-induced biodegradability, and excellent biocompatibility ( 38 , 39 ). Furthermore, nanoscale ZIF-8 can prevent drug extravasation and renal clearance during transport.…”