In vivoantibacterial activity ofGarcinia mangostanapericarp extract against methicillin-resistantStaphylococcus aureusin a mouse superficial skin infection model

Tatiya-aphiradee, Nitima; Chatuphonprasert, Waranya; Jarukamjorn, Kanokwan

doi:10.3109/13880209.2016.1172321

Cited by 24 publications

(23 citation statements)

References 42 publications

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“…Recent reports have revealed that α-mangostin from G. mangostana fruit possesses several medicinal properties, such as anti-microbial activity against bacteria [ 4 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ], anti-oxidant and neuro-protective activity [ 19 , 20 , 21 , 22 ], lipase inhibition [ 23 ], and anti-inflammatory and anti-cancer properties [ 24 ]. Biologically active molecules from medicinal plants are utilized as therapeutic agents, but most of the secondary metabolites do not exhibit optimum efficacy.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, by elucidating the structure of the active compound and the pharmacophores, the functional groups are considered as essential for the bioactivity of a compound. Since α- and non-α-mangostin xanthones have been shown to possess anti-bacterial activity, especially against Gram-positive bacteria [ 12 , 13 , 14 , 15 , 16 , 17 , 18 ], it is most probable that semi-synthetic analogs would be produced with enhanced anti-bacterial activity. In order to increase the bioactivity and antibacterial properties of α-mangostin, semi-synthetic modification of the compound was performed by several authors, to lead to more active compounds [ 25 , 26 , 27 , 28 , 29 ], with no excessive toxicity.…”

Section: Introductionmentioning

confidence: 99%

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Anti-Bacterial and Anti-Fungal Activity of Xanthones Obtained via Semi-Synthetic Modification of α-Mangostin from Garcinia mangostana

Narasimhan¹,

Maheshwaran²,

Abu‐Yousef

et al. 2017

Molecules

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The microbial contamination in food packaging has been a major concern that has paved the way to search for novel, natural anti-microbial agents, such as modified α-mangostin. In the present study, twelve synthetic analogs were obtained through semi-synthetic modification of α-mangostin by Ritter reaction, reduction by palladium-carbon (Pd-C), alkylation, and acetylation. The evaluation of the anti-microbial potential of the synthetic analogs showed higher bactericidal activity than the parent molecule. The anti-microbial studies proved that I E showed high anti-bacterial activity whereas I I showed the highest anti-fungal activity. Due to their microbicidal potential, modified α-mangostin derivatives could be utilized as active anti-microbial agents in materials for the biomedical and food industry.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anti-Bacterial and Anti-Fungal Activity of Xanthones Obtained via Semi-Synthetic Modification of α-Mangostin from Garcinia mangostana

Narasimhan¹,

Maheshwaran²,

Abu‐Yousef

et al. 2017

Molecules

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“…The minimum inhibitory concentration (MIC) was evaluated by the broth dilution method in MHB as described elsewhere (9). An inoculum of MSSA or MRSA (2 × 10 6 CFU/mL) was mixed with D. alatus extracts, α,βgurjunene, dipterocarpol, oxacillin, erythromycin, and tetracycline, and incubated at 37°C for 24 hours.…”

Section: Determination Of Minimum Inhibitory Concentration and Minimumentioning

confidence: 99%

“…The transepidermal water loss (TEWL) value was measured by DermaLab ® Combo (Hadsund, Denmark) and standardized at the level of 70 -75 g/m 2 h to normalize the skin damage of all mice. After that, a 10 µL-MRSA inoculum (1 × 10 8 CFU/mL) was applied to wounds (9). A 100-µL aliquot of each sample, i.e., 10% ethanol in propylene glycol (vehicle), 20 mg/mL of D. alatus twig/oleoresin/wood extract, 4 mg/mL of α,β-gurjunene, 1.1 mg/mL of dipterocarpol, or 160 µg/mL of tetracycline, was applied to the wound area daily for nine consecutive days.…”

Section: Tape-stripping Mouse Modelmentioning

confidence: 99%

Antibacterial and Wound Healing Activity of Dipterocarpus alatus Crude Extract Against Methicillin-resistant Staphylococcus aureus-induced Superficial Skin Infection in Mice

et al. 2019

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Background: The bark oil of Dipterocarpus alatus Roxb. ex G. Don (resin tree) has been used for the treatment of ulcerated wounds in Ayurvedic medicine. However, information is still scarce about D. alatus extract and its constituents. Objectives: This study aimed to examine the antibacterial and wound healing effects of D. alatus crude extracts against methicillinresistant Staphylococcus aureus (MRSA) in a superficial skin infection using a tape-stripping mouse model. Methods: Different parts (bark, leaves, twig, wood, and oleo-resin) of D. alatus were used for extraction. Crude extracts with two pure compounds (α,β-gurjunene and dipterocarpol) were examined for antibacterial activity against methicillin-susceptible S. aureus (MSSA) and MRSA in vitro. The in vivo antibacterial activity against MRSA was examined in a tape-stripping mouse model of superficial skin infection by evaluating transepidermal water loss (TEWL), colony counts from wound culture swabs, and H&E staining. Results: Dipterocarpus alatus twig extract showed the lowest MIC (250 µg/mL) and MBC (500 µg/mL) against MRSA among extracts from other D. alatus parts. In addition, α,β-gurjunene showed lower MIC (250 µg/mL) and MBC (250 µg/mL) against MRSA than dipterocarpol. In vivo, D. alatus twig extract significantly reduced the number of MRSA recovered from superficial wounds to levels comparable to the non-infected control group on the second day. Oleo-resin, D. alatus wood extract, α,β-gurjunene, and dipterocarpol also significantly reduced the number of MRSA. Moreover, D. alatus twig extract and dipterocarpol healed infected wounds at rates comparable to the non-infected control group. From H&E staining of wounds, fibrin accumulation and neutrophil infiltration were attenuated in mice treated with D. alatus twig extract and dipterocarpol. Conclusions: Dipterocarpus alatus twig extract and dipterocarpol possess antibacterial and wound healing properties against MRSAinduced superficial skin infection in mice.

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“…Currently there is a wide array of wound infection models in mice that can be used to evaluate new topical antimicrobials in vivo. These include the tape strip model (Kugelberg et al, 2005;Tatiya-aphiradee et al, 2016), incisional wound model (Lu et al, 2014), burn wound model (Liu et al, 2014;Turner et al, 2014), and a wound model that uses a biopsy punch (Zhao et al, 2010;Thompson et al, 2014;Chhibber et al, 2018). Though all have shown utility in assessing wound bacterial burden, few of the identified models lend themselves to evaluation of candidate topical antimicrobial therapies nor approximate wound healing in humans.…”

Section: Introductionmentioning

confidence: 99%

In situ Treatment With Novel Microbiocide Inhibits Methicillin Resistant Staphylococcus aureus in a Murine Wound Infection Model

et al. 2020

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Increased prevalence of antibiotic resistance in skin and soft tissue infections is a concerning public health challenge currently facing medical science. A combinatory, broad spectrum biocidal antiseptic has been developed ("ASP") as a topically applied solution to potential resistant and polymicrobial infected wounds that may be encountered in this context. The ASP-105 designate was evaluated in vitro by determining the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), against different strains of methicillin-resistant Staphylococcus aureus (MRSA), resulting estimates of which approximated the positive control (bacitracin). To evaluate in vivo microbicide efficacy, we utilized a murine full thickness wound model to study bacterial infection and wound healing kinetics. Mice were experimentally wounded dorsally and infected with bioluminescent MRSA. The infected wound was splinted, dressed and treated topically with either ASP-105, vehicle (-control), or bacitracin. Bacterial burden and wound healing was monitored using an in vivo imaging system and evaluation of biofilm formation using scanning electron microscopy of wound dressing. Treatment with ASP-105 significantly reduced bacterial burdens in the first 3 days of infection and inhibited MRSA biofilm formation on the surgical dressing. Notably, treatment with ASP-105 resulted in a sterilizing effect of any detectable MRSA in nearly all (80%; 4/5) of treatment group. All mice receiving vehicle control developed highly MRSA-luminescent and purulent wound beds as a result of experimental infection. The ASP-105 therapy facilitated natural healing in the absence of MRSA infection. Results of this study suggests that that the novel "ASP" combinatory topical antiseptic can be used directly in wounds as a potent, broadspectrum microbicide against drug resistant S. aureus without injury to the wound bed and impediment of natural restorative processes associated with wound healing. Further studies are warranted to test the effectiveness of this biocidal formulation against other recalcitrant bacterial and fungal pathogens in the context of serious wound infections, and to assess utility of use in both clinical and self-treat scenarios.

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In vivoantibacterial activity ofGarcinia mangostanapericarp extract against methicillin-resistantStaphylococcus aureusin a mouse superficial skin infection model

Abstract: GM-EtOH showed promising in vivo antibacterial activity against MRSA in a superficial skin infection model in mice. It is of interest to develop a topical formulation of GM-EtOH to further study its potential as a novel antibacterial agent.

Cited by 24 publications

References 42 publications

Anti-Bacterial and Anti-Fungal Activity of Xanthones Obtained via Semi-Synthetic Modification of α-Mangostin from Garcinia mangostana

Anti-Bacterial and Anti-Fungal Activity of Xanthones Obtained via Semi-Synthetic Modification of α-Mangostin from Garcinia mangostana

Antibacterial and Wound Healing Activity of Dipterocarpus alatus Crude Extract Against Methicillin-resistant Staphylococcus aureus-induced Superficial Skin Infection in Mice

In situ Treatment With Novel Microbiocide Inhibits Methicillin Resistant Staphylococcus aureus in a Murine Wound Infection Model

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