<i>In vivo</i> and <i>in vitro</i> digestible fractions in forage

Moir, K. W.

doi:10.1002/jsfa.2740220705

Cited by 19 publications

(11 citation statements)

References 17 publications

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“…Cell wall and digested cell wall were determined by the procedure of Moir (1971) in which the plant and the in vitro undigested residue from the Tilley & Terry (1963) procedure are sequentially extracted with acid-pepsin, ethanol and ammonium oxalate. After correcting for ash and residual protein (N x (6-25 the values were expressed as percentages in the original grass organic matter.…”

Section: Methodsmentioning

confidence: 99%

Thein vitrodigested cell wall and fermentation characteristics of grasses as affected by temperature and humidity during their growth

Moir

Wilson²,

Blight

1977

J. Agric. Sci.

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Total cell wall, in vitro digested cell wall and fermentation-gas production were determined in the separated tops and stubble of five tropical and two temperate grass species grown under controlled temperatures and humidities. As the day/night temperatures increased from 18/10 to 25/17 C C the total cell wall and in vitro digested cell wall increased. With a further increase to 32/24 °C the total cell wall increased, but not the in vitro digested cell wall. In vitro digested cell-wall values were also calculated from a previously derived relationship between in vitro digested cell wall and total cell wall. The differences between observed and calculated values increased (negatively) with increasing growth temperature suggesting that the in vitro digested cell wall was depressed with increasing growth temperatures, but the extent of this depression was small.In vitro gas production from the fermentation of plant tops or stubble in buffered rumen fluid for 24 h was significantly affected by growth temperature and humidity, apparently because of changes in chemical composition induced by the treatments. The volume of gas produced between 24 and 48 h fermentation times was appreciably lower from stubble than from plant tops and this was thought to be due to a higher resistance of part of the cell wall of stubble to digestion by rumen bacteria. Gas production in this period was slightly lower in the tops of grasses grown at the lower temperatures, but this could have been an indirect effect from an associated decrease in the total cell wall.TMTROTyTTPTTOTtf digestible dry matter, and they were led to conclude that the digestibility of cell-wall material was The digested cell wall of both tropical and tern-reduced at the higher temperatures. As the in vitro perate grass species when expressed as an amount digestible cell wall was not directly determined in digested per 100 g forage organic matter tends to this experiment, the conclusion is not unequivocal, be constant (Moir, 1972a(Moir, , 1974. Variation about For the direct determination of in vitro digestible the 'constant' digested cell wall could be due to cell wall, protein and ash corrections are required genetic variation within grass species, or to envir-on the cell-wall residue from extraction of the on mental effects on the cell wall during growth of plant, and on the similarly extracted residue from the plant. A knowledge of factors influencing the in vitro digestion (Moir, 19726). This is a formidable digested cell wall could help plant breeders in the task when there are a large number of plant samples selection of grasses with a high content of digestible for analysis and may be impracticable in pot cell wall.experiments that yield only small amounts of plant In a factorial experiment in which grasses were tissues. Moir (1976) has described a simple method grown at different controlled temperatures and for demonstrating differences between plants in the humidities, Wilson, Taylor & Dolby (1976) found properties of their cell walls by measuring the that t...

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Section: Methodsmentioning

confidence: 99%

Thein vitrodigested cell wall and fermentation characteristics of grasses as affected by temperature and humidity during their growth

Moir

Wilson²,

Blight

1977

J. Agric. Sci.

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“…Ash was determined by combustion in a furnace at 600 °C. Feed and faeces samples were sequentially extracted with acid-pepsin, ethanol and hot water by the procedure of Moir (1971). Cell wall was the residue from these extractions less ash and unextracted protein.…”

Section: Analysesmentioning

confidence: 99%

Effects of induced, subclinical phosphorus deficiency on feed intake and growth of beef heifers

1982

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Hereford heifers were fed a diet (7-0 MJ ME/kg D.M.) containing 0-09 % phosphorus but complete with regard to other nutrient constituents. Only after 19 weeks wore the effects of subclinical aphosphorosis evident in the form of reduction in feed intake, cessation of weight gain, increased feed conversion and decreases in tho concentration of inorganic phosphorus in blood and saliva. Heifers supplemented daily with 12 g phosphorus sustained a weight gain of about 0-2 kg/day over 62 weeks as did other supplemented heifers whose feed intake was restricted to that of tho low phosphorus treatment. The effects of phosphorus supplementation were thus demonstrated without being confounded by concomitant increases in feed intake.Phosphorus supplementation resulted in significantly lowered apparent crude protein digestibility and a slight increaso in cell wall digestibility.

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“…Dry-matter disappearance from 48 h digestion of oesophageal extrusa in nylon bags suspended in the rumen was determined by the method of Mehrez & 0rskov (1977). In vitro digestible organic matter (OM) by the method of Tilley & Terry (1963) was fractionated into digestible cell walls by the method of Moir (1971), but adapted to the equipment described by Moir (1982 a).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of pasture for beef cattle from measurements of cell wall in separated leaf and stem fractions

Moir

Ebersohn

1983

J. Agric. Sci.

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SUMMARYThree pastures, pangola–clover, setaria, and tropical grass–legume, were grazed for a 9-month period by 12 steers of initial live weight of 199 kg on each. For the first 5 months live-weight gains were, respectively, 1·2, 0·76 and 0·43 kg per day, mostly higher than could be expected for estimated dietary energy concentrations of 10·1, 8·9 and 8·7 MJ of metabolizable energy (ME) per kg of dry matter. The high live-weight gains were not explained satisfactorily by dry-matter intakes as measured from faeces voided and dietary digestible dry matter, but were possible if published values for the requirements of ME for growth and fattening were too high. Cell wall, in vitro digested cell wall and nitrogen in separated leaf and stem fractions were useful indices for evaluating pasture for grazing cattle, but they did not predict live-weight gain.

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In vivo and in vitro digestible fractions in forage

Cited by 19 publications

References 17 publications

Thein vitrodigested cell wall and fermentation characteristics of grasses as affected by temperature and humidity during their growth

Thein vitrodigested cell wall and fermentation characteristics of grasses as affected by temperature and humidity during their growth

Effects of induced, subclinical phosphorus deficiency on feed intake and growth of beef heifers

Evaluation of pasture for beef cattle from measurements of cell wall in separated leaf and stem fractions

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