2020
DOI: 10.1101/2020.05.13.092551
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In vivoanalysis ofTrypanosoma cruzipersistence foci at single cell resolution

Abstract: 247 23 Text: 4892 24 25 2 ABSTRACT Infections with Trypanosoma cruzi are usually life-long despite 26 generating a strong adaptive immune response. Identifying the sites of parasite 27persistence is therefore crucial to understand how T. cruzi avoids immune-mediated 28 destruction. However, this is a major technical challenge because the parasite burden 29 during chronic infections is extremely low. Here, we describe an integrated approach 30 involving comprehensive tissue processing, ex vivo imaging, and conf… Show more

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Cited by 10 publications
(23 citation statements)
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“…Visible light towards the red end of the spectrum (617 nm in this case) has greater tissue penetration due to reductions in the absorbance and scattering of the emitted light. Using these systems, chronic T. cruzi infections in mice can be monitored for more than one year, with a limit of detection close to 100 parasites using in vivo imaging [100], and less than 20 parasites when ex vivo imaging is subsequently used to examine mouse tissue [103]. With in vivo imaging, there is a linear relationship between the parasite burden (above 1000 parasites) and whole-body bioluminescence intensity [99].…”
Section: Highly Sensitive In Vivo Bioluminescence Imaging and Its Appmentioning
confidence: 99%
See 3 more Smart Citations
“…Visible light towards the red end of the spectrum (617 nm in this case) has greater tissue penetration due to reductions in the absorbance and scattering of the emitted light. Using these systems, chronic T. cruzi infections in mice can be monitored for more than one year, with a limit of detection close to 100 parasites using in vivo imaging [100], and less than 20 parasites when ex vivo imaging is subsequently used to examine mouse tissue [103]. With in vivo imaging, there is a linear relationship between the parasite burden (above 1000 parasites) and whole-body bioluminescence intensity [99].…”
Section: Highly Sensitive In Vivo Bioluminescence Imaging and Its Appmentioning
confidence: 99%
“…parasites using in vivo imaging [100], and less than 20 parasites when ex vivo imaging is subsequently used to examine mouse tissue [103]. With in vivo imaging, there is a linear relationship between the parasite burden (above 1000 parasites) and whole-body bioluminescence intensity [99].…”
Section: Highly Sensitive In Vivo Bioluminescence Imaging and Its Appmentioning
confidence: 99%
See 2 more Smart Citations
“…The well‐studied parasite strain CL Brener is also commonly detected in the skin of BALB/c mice 38 but only sporadically in other sites, for example skeletal muscle, lung, adipose. More virulent parasite strains (eg Brazil, Colombiana, Tulahuen, VFRA) and certain mice (eg C3H) are associated with more disseminated infections, including heart and skeletal muscle localization 29,30,170‐175 . There have been few robust data on the relevant cell types within any of these chronically infected tissues, but recent in vivo imaging analysis at single cell resolution revealed smooth muscle cells as the most frequent targets in the colon 175 …”
Section: Stage 3: the 1% And The Chronic Host‐parasite Equilibriummentioning
confidence: 99%