<i>In vivo</i> abnormal keratinization in Darier‐White's disease as viewed by real‐time confocal imaging

González, Salvador; Rubinstein, Gennady; Mordovtseva, V. V.; Rajadhyaksha, Milind; Anderson, R. Rox

doi:10.1111/j.1600-0560.1999.tb01797.x

Cited by 44 publications

(33 citation statements)

References 12 publications

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“…Further, well-established histological features of psoriasis and other inflammatory skin lesions can be visualized with this technique, including characteristic PMN accumulations. It has been shown that these RCM images correlated highly with histology (27)(28)(29)(30)(31).…”

Section: Introductionmentioning

confidence: 95%

Establishing the dynamics of neutrophil accumulation in vivo by reflectance confocal microscopy

Wolberink

Peppelman

Kerkhof

et al. 2014

Experimental Dermatology

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Reflectance confocal microscopy (RCM) is an imaging tool, which visualizes the epidermal skin layers in vivo with a cellular resolution. Neutrophil accumulation is a characteristic feature in psoriasis and is thought to play a role in the pathophysiology of psoriasis. Until now, imaging of neutrophil accumulation in vivo is not performed. We evaluated the dynamics of neutrophil migration in active psoriatic lesions by non-invasive RCM imaging. Additionally, we evaluated the time phasing and duration of neutrophil trafficking. We performed RCM imaging prior to the start of topical treatment and for seven consecutive days with a 24-h time interval at the Radboud University Medical Center, Nijmegen, the Netherlands. Twelve psoriatic lesions in three patients with a severe exacerbation of psoriasis were included. The four most active lesions were selected in each patient based on the highest degree of redness, induration and expansion in the previous 2 weeks. In all lesions, a cyclic pattern of neutrophil migration was observed, consisting of squirting papillae, transepidermal migration, accumulation in the stratum spinosum, accumulation in the stratum corneum and degeneration of the abscesses. The time interval of a neutrophil-trafficking cycle was 5-7 days and showed a synchronic time phasing. This study is the first to establish the dynamics and time phasing of neutrophil migration in vivo in psoriatic lesions. Previously reported theories were confirmed by these novel in vivo data. RCM might distinguish between active or chronic psoriatic areas, which might contribute to new insights into the pathogenesis of psoriasis.

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Section: Introductionmentioning

confidence: 95%

Establishing the dynamics of neutrophil accumulation in vivo by reflectance confocal microscopy

Wolberink

Peppelman

Kerkhof

et al. 2014

Experimental Dermatology

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“…González et al [14] presented confocal images of dyskeratosis in the form of 7-10 µm cells in the stratum corneum, some of them containing a pyknotic nucleus, called Darier's grains. Below the stratum corneum the so-called 'corps ronds' 20-25 µm in diameter were found.…”

Section: Darier's Diseasementioning

confidence: 99%

Review paper The use of reflectance confocal microscopy in selected inflammatory skin diseases

Białek-Galas¹,

Wielowieyska-Szybińska²,

Dyduch³

et al. 2015

pjp

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Reflectance confocal microscopy is a modern, non-invasive diagnostic method that enables real-time imaging of the epidermis and upper layers of the dermis with nearly histological precision and high contrast. The application of this technology to skin imaging during the last years has resulted in progress of dermatological diagnosis, providing virtual access to living skin, without the need for conventional histopathology. The presented method potentially has broad application in the diagnosis of skin diseases. This article provides a summary of the latest reports and previous achievements in the field of reflectance confocal microscopy. General characteristics of confocal images in selected inflammatory skin diseases are presented.

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“…Studies comparing and identifying structures imaged by conventional histological sectioning and the confocal laser scanning microscopy are available [15][16][17][18]. The microscope used in this study consisted of a light source, pin holes, a movable lens, a beam splitter and a photo detector.…”

Section: Study Design Of the Determination Of The Number Of Dermal Pamentioning

confidence: 99%

Topical Activity of Ascorbic Acid: From in vitro Optimization to in vivo Efficacy

Raschke

Koop²,

Düsing³

et al. 2004

Skin Pharmacol Physiol

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We present here a new cosmetic formula system containing 3% ascorbic acid based on an optimized oil-in-water (O/W) emulsion. This formulation demonstrated a good long-term stability of the active ingredient and also of the emulsion itself. It could be deduced from in vitro release studies that this O/W emulsion enabled a better release of the hydrophilic active agent than an alternative W/O emulsion. By measuring the ultraweak photon emission, which is a well-established parameter for the oxidative stress in the skin, the high in vivo antioxidant capacity of 3% ascorbic acid was demonstrated after 1 week of product application. This placebo-controlled study also proved that ascorbic acid in an O/W cream reduced oxidative stress in human skin significantly better than the derivative sodium ascorbyl-2-phosphate, a more stable vitamin C replacement commonly used in cosmetic formulations. With increasing age, the number of papillae in the epidermal-dermal junction zone in human skin are reduced. This implies a possible consequence of reduced mechanical resistance of the skin and impaired supply of the epidermis with nutrients. In a 1-month placebo-controlled study on 25 human volunteers, a significant increase in the number of dermal papillae after application of the 3% ascorbic acid cream was demonstrated, using a confocal laser scanning microscope. Fine lines and wrinkles are a characteristic sign of aged and especially photo-aged skin. Application of 3% ascorbic acid in a 12-week placebo-controlled usage study indicated a significant reduction of facial wrinkles. Altogether, 3% ascorbic acid in a cosmetic O/W emulsion has been shown to be appropriately stable and to enable a good release of the active agent in vitro as a precondition for a high efficacy in vivo. Application in vivo resulted in a significant reduction of oxidative stress in the skin, an improvement of the epidermal-dermal microstructure and a reduction of fine lines and wrinkles in aged skin. These results were received within a relatively short period of time of product application.

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In vivo abnormal keratinization in Darier‐White's disease as viewed by real‐time confocal imaging

Cited by 44 publications

References 12 publications

Establishing the dynamics of neutrophil accumulation in vivo by reflectance confocal microscopy

Establishing the dynamics of neutrophil accumulation in vivo by reflectance confocal microscopy

Review paper The use of reflectance confocal microscopy in selected inflammatory skin diseases

Topical Activity of Ascorbic Acid: From in vitro Optimization to in vivo Efficacy

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