<i>In vitro</i>toxicity of indoor moulds from Slovak dwellings

Piecková, Elena; Kolláriková, Zuzana

doi:10.2495/etox080241

Cited by 4 publications

(6 citation statements)

References 14 publications

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“…On the other hand, Alternaria alternata , a major allergen and one of the most common fungi often associated with asthma development, persistence, and exacerbation [ 67 , 68 , 69 ], was found abundant in HNM and HVM. In addition, Chaetomium globosum (abundant in both home types) is a fungus with type I and III allergens associated with skin and nail infections in humans and infrequently causes cerebral and systemic infections in immunocompromised individuals [ 70 , 71 , 72 , 73 ]. In a pilot study, Andersen et al investigated the air quality in different houses (with no visible fungi) in Denmark.…”

Section: Discussionmentioning

confidence: 99%

Indoor Bacterial and Fungal Burden in “Moldy” versus “Non-Moldy” Homes: A Case Study Employing Advanced Sequencing Techniques in a US Metropolitan Area

Chauhan,

Higgins Jones,

Banerjee

et al. 2023

Pathogens

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The presence of fungi in the indoor environment is associated with allergies and other respiratory symptoms. The aim of this study was to use sequencing and molecular methods, including next-generation sequencing (NGS) approaches, to explore the bacterial and fungal communities and their abundance in the indoor environment of houses (n = 20) with visible “moldy” (HVM) and nonvisible “non-moldy” (HNM) in Memphis, TN, USA. Dust samples were collected from air vents and ground surfaces, and the total DNA was analyzed for bacteria and fungi by amplifying 16S rRNA and ITS genes on the Illumina Miseq. Results indicated that Leptosphaerulina was the most abundant fungal genus present in the air vent and ground samples from HNM and HVM. At the same time, the most abundant bacterial genera in the air vent and ground samples were Propionibacterium and Streptococcus. The fungi community diversity was significantly different in the air vent samples. The abundance of fungal species known to be associated with respiratory diseases in indoor dust samples was similar, regardless of the visibility of fungi in the houses. The existence of fungi associated with respiratory symptoms was compared with several parameters like dust particulate matter (PM), CO2 level, temperature, and humidity. Most of these parameters are either positively or negatively correlated with the existence of fungi associated with respiratory diseases; however, none of these correlations were significant at p = 0.05. Our results indicate that implementing molecular methods for detecting indoor fungi may strengthen common exposure and risk assessment practices.

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Section: Discussionmentioning

confidence: 99%

Indoor Bacterial and Fungal Burden in “Moldy” versus “Non-Moldy” Homes: A Case Study Employing Advanced Sequencing Techniques in a US Metropolitan Area

Chauhan,

Higgins Jones,

Banerjee

et al. 2023

Pathogens

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“…This species is recognised as a cellulolytic and/or endophytic fungus and produces secondary metabolites and mycotoxins [37,38]. Chaetomium globosum has also been reported as an air contaminant causing adverse health effects such as allergies and as causal agent of human fungal infections [39,40]. These fungi are widespread and commonly live on cellulose, and are found on wood, compost, sheet rock paper, straw [41], and similar materials such as biodeteriorated paint film [42].…”

Section: Microbiologic Analysismentioning

confidence: 99%

Application of imaging spectroscopy, SEM-EDS and DNA analysis for monitoring the preservation status of a manuscript of the first decade of 19th century

Kokla,

Batrinou,

Papatheodorou

et al. 2023

Eur. Phys. J. Plus

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This paper presents an interdisciplinary approach on the preservation status of a manuscript made in the early nineteenth century on paper support. This approach was based on the analysis of the pigments and inks, as well as the damage of the manuscript, which was provoked by biological attack. This analysis was accomplished with the application of innovating techniques such as imaging spectroscopy using high resolution cameras and DNA analysis. The use of new technologies from diverse science can provide useful information on the inks and pigments as well as the type of biological agents found in the manuscript. Furthermore, apart from the knowledge about the inks and pigments, important elements can be retrieved on their preservation status. The aim of this research is the combined use of the detected information on the types of inks and pigments with the biological investigation in order to trace damage caused by biological attack and to investigate the causative agent of this attack. For this reason, imaging techniques, SEM/EDS analysis were applied to the inks and pigments as well as a microbiologic and molecular analysis of the fungi found on the pigments of manuscript.

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“…Our previous study was focused on comparing the effects of metabolites isolated from four different strains of Stachybotrys chartarum [17] in vitro. The results showed that metabolites from all strains were toxic and they differ only in extent of the evoked changes.…”

Section: Discussionmentioning

confidence: 99%

In vitrotoxicity of indoor fungi from dwellings in Slovakia: testing on the isolated lung cells

Kovačíková

Tátrai

Piecková

et al. 2008

WIT Transactions on Ecology and the Environment

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The lung is the target organ of multiple aggressions due to environmental noxious substances, indoor pollution, occupational hazards and personal risk such as cigarette smoke. Metabolites produced by different fungus species can also be present the inhaled air. Their effects are not frequently studied in the lung or in the lung cells. Our study focused on the effects of metabolites (both exoand endometabolites) produced by Aspergillus ustus, Aspergillus versicolor, Penicillium chrysogenum and Stachybotrys chartarum isolated from dwellings in Slovakia. Their effects were studied in vitro on alveolar macrophages and epithelial type II cells isolated from Wistar rats and Clara cells isolated from mice. Alveolar macrophages represent a free living population in the alveolar spaces and play an important role in maintaining clean and sterile alveoli; they contribute also to the production of proinflammatory cytokines. Epithelial type II cells play a critical role in preserving the functional integrity of the alveolar surface and they also produce cytokines. The effects of metabolites were evaluated by estimating their cytotoxicity, the activity of lysosomal enzyme acid phosphatase in alveolar macrophages. Lectins were used for studying the changes on cell surface. The production of cytokines (Monocyte Chemoatractant Protein 1-MCP-1 and Tumor Necrosis Factor α -TNF-α) was also measured. The effects of metabolites were dose dependent, the highest toxicity was evoked by Stachybotrys chartarum metabolites.

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In vitrotoxicity of indoor moulds from Slovak dwellings

Cited by 4 publications

References 14 publications

Indoor Bacterial and Fungal Burden in “Moldy” versus “Non-Moldy” Homes: A Case Study Employing Advanced Sequencing Techniques in a US Metropolitan Area

Indoor Bacterial and Fungal Burden in “Moldy” versus “Non-Moldy” Homes: A Case Study Employing Advanced Sequencing Techniques in a US Metropolitan Area

Application of imaging spectroscopy, SEM-EDS and DNA analysis for monitoring the preservation status of a manuscript of the first decade of 19th century

In vitrotoxicity of indoor fungi from dwellings in Slovakia: testing on the isolated lung cells

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