In VitroSynergistic Effect ofPsidium guineense(Swartz) in Combination with Antimicrobial Agents against Methicillin-ResistantStaphylococcus aureusStrains

Fernandes, Tiago Gomes; Mesquita, Amanda Rafaela Carneiro de; Randau, Karina Perrelli; Franchitti, Adelisa Alves; Ximenes, Eulália Azevedo

doi:10.1100/2012/158237

Cited by 20 publications

(18 citation statements)

References 21 publications

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“…The roots of P. guineense have been traditionally used in Brazil as antidiarrheals and diuretics, and the bark is used in tanneries due to its high tannin content (Rodrigues and Carvalho, 2001). A study by González et al (2005) potential of the pulp of P. guineense fruit and Fernandes et al (2012) realized a growth inhibitory effect of P. guineense extract on antimicrobial agents against methicillin-resistant Staphylococcus aureus strains due for it combination with beta-lactams, carbapenems and fluoroquinolones in antibiotic, corroborating the hypothesis that P. guineense extract have synergistic effect in antimicrobial activity.…”

Section: Introductionmentioning

confidence: 50%

Antibacterial activity of tannins from Psidium guineense Sw. (Myrtaceae)

Rodrigues¹,

Ferreira²,

Mendes³

et al. 2014

J. Med. Plants Res.

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The aim of this study was to quantify and evaluate the antibacterial activity of tannins extracted from leaves of Psidium guineense, obtained using two different isolation methods, against Staphylococcus aureus and Pseudomonas aeruginosa. The first extraction method was used to isolate condensed tannins (Method A) and the second to isolate mixtures rich in esters of gallic acid and glucose (Method B). Both extraction methods yielded high concentrations of tannins, with 312 and 257 mg/g of dry material obtained using Methods A and B, respectively. These compounds formed halos of growth inhibition in S. aureus and P. aeruginosa cultures. Tannins isolated by method B gave rise to larger inhibition halos than those isolated by method A. Analysis of the antibacterial activity of tannins isolated by method B revealed a minimum inhibitory and bactericidal concentration of 256 μg/ml for S. aureus and 512 μg/ml for P. aeruginosa. These results demonstrate that P. guineense is a promising source of pharmacologically active tannin molecules, and further studies are therefore necessary to determine the toxicity of the plant and ensure its safe use for animal and human health.

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Section: Introductionmentioning

confidence: 50%

Antibacterial activity of tannins from Psidium guineense Sw. (Myrtaceae)

Rodrigues¹,

Ferreira²,

Mendes³

et al. 2014

J. Med. Plants Res.

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“…It should be noted, however, that in vitro data should not be directly extrapolated to the clinic, since in vitro tests do not consider several factors, such as variations in pH, antimicrobial substantivity, and microbiota composition, which can interfere in the clinical efficiency of these products. 25,26…”

Section: Discussionmentioning

confidence: 99%

Microbial susceptibility of bacteria and fungi against extracts of Psidium guajava L.: in vitro evaluation

et al. 2016

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Aim:The present study aimed to evaluate the in vitro antimicrobial activity of hydroalcoholic and aqueous extracts from leaves of P. guajava on four bacterial and two fungal samples to determine the range of minimum inhibitory concentration of the extracts. Material and Methods: The antimicrobial activity of extracts of different polarities was compared using the agar dilution method. Differences in susceptibility to the active ingredient could be observed. Results: The results showed that the hydroalcoholic extract was active against Escherichia coli (1024 µg/ml) and Bacillus cereus (900 µg/ml). The aqueous extract showed no antimicrobial activity. Conclusions: Despite demonstrating antimicrobial efficiency, the P. guajava extract may be insufficient or may require a higher concentration to be fully effective. Uniterms: Psidium. Products with antimicrobial action. Traditional medicine.

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“…46 Moreover, the process for developing a new drug is very expensive which is not possible in most developing countries and the use of a new drug also has risks of unknown side effects. Here in the current study, the neem limonoids plus two first generation cephalosporin antibiotics recorded significant synergistic effect.…”

Section: Discussionmentioning

confidence: 99%

Nimbolide from Azadirachta indica and its derivatives plus first-generation cephalosporin antibiotics: a novel drug combination for wound-infecting pathogens

et al. 2015

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The shortage of effective drugs against wound infecting pathogens poses a serious public health threat. Combination treatment may represent a good choice for treating infections caused by these pathogens. The aim of the present study was to evaluate the in vitro efficacy of nimbolide, desacetylnimbin isolated from Azadirachta indica and the amide derivatives of nimbolide in combination with first generation cephalosporin antibiotics against major wound associated bacterial pathogens. The antibacterial activity of the compounds and antibiotics were studied by calculating minimum inhibitory concentration and minimum bactericidal concentration. The checkerboard and time-kill assay were used to evaluate the interactions between the compounds and antibiotics. Nimbolide recorded highest antimicrobial activity against all tested strains followed by desacetylnimbin but the amide derivatives of nimbolide were found to be less active. The MICs of test compounds ranged from 64 to 2000 µg/mL. In the checkerboard test, nimbolide and its derivatives markedly reduced the MIC values of the antibiotics. Significant synergistic effect was recorded by nimbolide as well as desacetylnimbin in combination with antibiotics and this combination recorded significant reduction of the colony forming units (CFUs) in time kill assay and the maximum reduction was recorded between 4 to 12 h. The above combination was also found to be effective against methicillin-resistant Staphylococcus aureus (MRSA), an important drug resistant bacterium. The cytotoxicity of the compounds was tested against H9c2 and recorded no toxicity up to 200 µM. In summary, the combination of nimbolide/desacetylnimbin and antibiotics demonstrated synergistic activity against the major wound associated bacteria tested in this study. Furthermore, these compounds may potentially widen the therapeutic window of antibiotics, suggesting that these combinations could be used clinically to control infections caused by wound pathogens after in vivo experiments.

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In VitroSynergistic Effect ofPsidium guineense(Swartz) in Combination with Antimicrobial Agents against Methicillin-ResistantStaphylococcus aureusStrains

Cited by 20 publications

References 21 publications

Antibacterial activity of tannins from Psidium guineense Sw. (Myrtaceae)

Antibacterial activity of tannins from Psidium guineense Sw. (Myrtaceae)

Microbial susceptibility of bacteria and fungi against extracts of Psidium guajava L.: in vitro evaluation

Nimbolide from Azadirachta indica and its derivatives plus first-generation cephalosporin antibiotics: a novel drug combination for wound-infecting pathogens

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