<i>IN VITRO</i> STUDIES WITH HYALURONIDASE

Tolksdorf, Sibylle; Cassidy, James W.; McCready, Marion H.; McCullagh, D. Roy

doi:10.1111/j.1749-6632.1950.tb54000.x

Cited by 11 publications

(3 citation statements)

References 4 publications

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“…All of the assays were standardized to the turbidity reducing unit (TRU) with commercial hyaluronidase (Sigma type VI hyaluronidase) as a standard. One TRU of hyaluronidase is defined as the amount of enzyme that will decrease the turbidity producing capacity of 0.2 mg HA to that of 0.1 mg HA in 30 min at 37°C [16]. Protein concentrations were measured with the detergent‐compatible Micro BCA assay (Pierce, Rockford, IL, USA).…”

Section: Methodsmentioning

confidence: 99%

Purification and microsequencing of hyaluronidase isozymes from human urine

et al. 1997

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We recently cloned the major hyaluronidase of human plasma, which we termed HYAL1. All hyaluronidase activity could be purified from human urine on an anti-HYALl monoclonal antibody column. However, urine contains two hyaluronidases of 57 kDa and 45 kDa, whereas plasma only contains the 57 kDa activity. Microsequencing confirmed that both urinary isozymes have N-termini identical to plasma hyaluronidase, but a second N-terminus was found in the smaller isozyme, apparently derived from the terminal 25 amino acids of HYAL1, at the C-terminus. The two polypeptides of the 45 kDa isozyme resulting from endoproteolytic cleavage of the 57 kDa isoform are presumably linked by disulfide bonds. Sperm contains two isozymes of the testicular hyaluronidase, PH-20, and the lower molecular weight isozyme is believed to be an endoproteolytically processed form of the larger protein.Analogously to PH-20, the smaller isozyme of HYAL1 is likely to be a proteolytically processed product of the larger isozyme.

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Section: Methodsmentioning

confidence: 99%

Purification and microsequencing of hyaluronidase isozymes from human urine

et al. 1997

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“…Estimation of hyaluronidase. This was determined by a method essentially similar to that of Tolksdorf, McCready, McCullagh & Schwenk (1949), a lyophilized preparation of hyaluronidase being used as standard. Hyaluronic acid was prepared from vitreous humour of the ox by the method described by Seastone (1939).…”

Section: Methodsmentioning

confidence: 99%

The separation and properties of the neurotoxins from the venom of the tiger snake Notechis scutatus scutatus

Doery

1958

Biochemical Journal

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DEHYDROGENASES IN CHICK EMBRYO 535 4. Although LDH activity per milligram of protein in the developing brain and skeletal muscle did not increase during early development there was a slight increase of LDH in the heart and a threefold temporary increase in the liver between 12 and 16 days of incubation. 5. MDH activity per milligram of protein increased steadily throughout early development in the brain, heart and muscle, but showed a temporary twofold increase in the liver between 12 and 16 days of incubation. 6. The levels of LDH and MDH activity of embryonic organs are compared with those of the adult hen. I wish to thank Professors A. Haddow and F. Bergel for their interest and encouragement in this work.

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“…Enzyme assays: substrate. Hyaluronic acid was prepared from the vitreous humor of beef eyes by the method of Seastone (1939) or from human umbilical cords by the method of Tolksdorf, Cassidy, McCready, and McCullagh (1950).…”

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The Production of Hyaluronidase by Lancefield's Group B Streptococci

Gochnauer

Wilson

1951

J Bacteriol

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The relationship of hyaluronidase produced by a microorganism to the virulence and invasiveness of the organism has been widely investigated (Meyer, 1947). The discovery by McClean et al. (1943) that Clostridium perfringens growing in tissues produced hyaluronidase gave impetus to these investigations. Since, during the course of our studies on bovine mastitis, Streptococcus agalactiae (Lancefield's Group B) has been found to be the species of streptococcus most frequently associated with clinical and subelinical mastitis in the area of Madison, the following work was undertaken to determine the extent of hyaluronidase production by these cocci and, also, the relation of the production of hyaluronidase to various environmental factors. Few studies of hyaluronidase production by the various serological groups of streptococci have been concerned with enzymatic activity by cultures in Group B. McClean (1941) reported production of hyaluronidase by these streptococci; Pierce (1947) found 3 of 11 Group B streptococci isolated from human beings to have activity; Russell and Sherwood (1949) studied a series of cultures from human beings and reported that Group B streptococci exhibited the highest percentage of hyaluronidase producing strains. Sellers (1949) reported that all of 54 strains of Group B streptococci isolated during the course of a study on bovine mastitis produced in vitro a low titer of hyaluronidase. MATERIALS AND METHODS Cultures. All the bovine cultures used were obtained from blood agar streak plates used to confirm the Hotis test for mastitis streptococci. These tests were conducted by G. R. Spencer and J. Simon of the Department of Veterinary Science as part of their studies of the treatment of mastitis. Each isolate was obtained from one-quarter of the udder of a cow, since previous work had indicated that a single species of streptococcus was usually found in a given quarter. The cultures were picked from the blood agar plates into tryptose broth for purposes of identification and hyaluronidase assay. The cocci were identified on the basis of one or more of the following reactions: (1) their action in hippurate and aesculin (Soulides, 1942); (2) the Lancefield precipitin reaction; (3) the CAMP reaction (Christie, Atkins, and Munch-Peterson, 1944). Cultures which fermented hippurate without the hydrolysis of aesculin, which gave positive precipitin

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IN VITRO STUDIES WITH HYALURONIDASE

Cited by 11 publications

References 4 publications

Purification and microsequencing of hyaluronidase isozymes from human urine

Purification and microsequencing of hyaluronidase isozymes from human urine

The separation and properties of the neurotoxins from the venom of the tiger snake Notechis scutatus scutatus

The Production of Hyaluronidase by Lancefield's Group B Streptococci

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