<i>In vitro</i> studies on the influence of surface modification of Ni–Ti alloy on human bone cells

Chrzanowski, Wojciech; Neel, Ensanya A. Abou; Armitage, David; Zhao, Xin; Knowles, Jonathan C.; Salih, Vehid

doi:10.1002/jbm.a.32646

Cited by 24 publications

(21 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[26][27][28][29][30][31] In contrary, other studies demonstrated that despite a high nickel surface content and high nickel release rate, cell response was not drastically aected. 1,9,21,32,33 To modulate cell response, simple surface treatments, such as thermal oxidation or polishing, were shown to be as eective as more expensive and complex treatments such as diamond-like coating (DLC).…”

Section: Introductionmentioning

confidence: 92%

“…[16][17][18][19][20] The manipulation of only surface characteristics, such as local stiness, topography, surface charge, and structure, makes it possible to trigger or inhibit biological action, e.g., cell dierentiation and mineralization. [21][22][23][24][25] Good biocompatibility and/or osseointegration remain a challenge for nickel-titanium. The improvement of the biocompatibility is the primary aim of this article.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biocompatible, Smooth, Plasma-Treated Nickel–Titanium Surface – An Adequate Platform for Cell Growth

et al. 2011

View full text Add to dashboard Cite

High nickel content is believed to reduce the number of biomedical applications of nickel-titanium alloy due to the reported toxicity of nickel. The reduction in nickel release and minimized exposure of the cell to nickel can optimize the biocompatibility of the alloy and increase its use in the application where its shape memory effects and pseudoelasticity are particularly useful, e.g., spinal implants. Many treatments have been tried to improve the biocompatibility of Ni-Ti, and results suggest that a native, smooth surface could provide sufficient tolerance, biologically. We hypothesized that the native surface of nickel-titanium supports cell differentiation and insures good biocompatibility. Three types of surface modifications were investigated: thermal oxidation, alkali treatment, and plasma sputtering, and compared with smooth, ground surface. Thermal oxidation caused a drop in surface nickel content, while negligible chemistry changes were observed for plasma-modified samples when compared with control ground samples. In contrast, alkali treatment caused significant increase in surface nickel concentration and accelerated nickel release. Nickel release was also accelerated in thermally oxidized samples at 600 °C, while in other samples it remained at low level. Both thermal oxidation and alkali treatment increased the roughness of the surface, but mean roughness R(a) was significantly greater for the alkali-treated ones. Ground and plasma-modified samples had 'smooth' surfaces with R(a)=4 nm. Deformability tests showed that the adhesion of the surface layers on samples oxidized at 600 °C and alkali treatment samples was not sufficient; the layer delaminated upon deformation. It was observed that the cell cytoskeletons on the samples with a high nickel content or release were less developed, suggesting some negative effects of nickel on cell growth. These effects were observed primarily during initial cell contact with the surface. The most favorable cell responses were observed for ground and plasma-sputtered surfaces. These studies indicated that smooth, plasma-modified surfaces provide sufficient properties for cells to grow.

show abstract

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Biocompatible, Smooth, Plasma-Treated Nickel–Titanium Surface – An Adequate Platform for Cell Growth

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The absorbance was measured at 570 nm using BioRadmicroplate reader. 36 Each experiment was performed in triplicate and the average value was statistically represented.…”

Section: Mtt Assaymentioning

confidence: 99%

Silver incorporated antibacterial, cell compatible and bioactive titania layer on Ti metal for biomedical applications

Rajendran

Pattanayak

2014

RSC Adv.

View full text Add to dashboard Cite

Surface modification of titanium and titanium alloy is one of the attractive methods to improve the biological affinity of orthopaedic and dental devices. Although osteointegration is enhanced by surface modifications, bacterial related infections sometimes lead to implant failure and secondary surgery. In the present study, attempts were made to incorporate antimicrobial agent silver into titanium metal pre-treated with H 2 O 2 . Fine nano network structures of hydrated titania uniformly formed by the H 2 O 2 treatment were decorated with silver particles of 5-10 nm by subsequent AgNO 3 , and, these silver particles remained stable over titania network even after heat treatment. Antibacterial study of titanium metal subjected to H 2 O 2 -AgNO 3 showed zone of inhibition in Staphylococcus aureus compared to H 2 O 2 pre-treated and untreated control specimen. Steady release of silver from thus treated titanium metals into simulated body fluid indicates that these silver particles are released as silver ions and are responsible for the antibacterial activity. About 99.7% bacterial killing efficiency was observed for 6-8 ppm (1mM AgNO 3 ) silver containing Ti surface is optimised as the highest tolerable silver limit. The cytotoxicity assay and cell adhesion study on MG63 osteosarcoma cell lines confirmed the present silver concentration does not show any toxicity. Further, bone like apatite formation of chemically and heat-treated titanium in simulated body fluid indicates this surface modification method could be suitable in various medical devices.

show abstract

“…30 However, in previous research, those differentiated cell lineages could also be achieved by applying various chemical differentiation factors, including dexamethasone or other growth factors, such as bone morphogenetic proteins. 31 However, at present, it is valuable to know that topography alone can elicit such effects with sufficient proof. 29 Oh et al noted that the osteoinductive ability of nanotubes on BMMSCs was enhanced as the dimension was increased from 30 nm to 100 nm.…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Liu³

et al. 2012

IJN

View full text Add to dashboard Cite

Background:The topography of an implant surface can serve as a powerful signaling cue for attached cells and can enhance the quality of osseointegration. A series of improved implant surfaces functionalized with nanoscale structures have been fabricated using various methods. Methods: In this study, using an H 2 O 2 process, we fabricated two size-controllable sawtooth-like nanostructures with different dimensions on a titanium surface. The effects of the two nanosawtooth structures on rat bone marrow mesenchymal stem cells (BMMSCs) were evaluated without the addition of osteoinductive chemical factors. Results: These new surface modifications did not adversely affect cell viability, and rat BMMSCs demonstrated a greater increase in proliferation ability on the surfaces of the nanosawtooth structures than on a control plate. Furthermore, upregulated expression of osteogenicrelated genes and proteins indicated that the nano-sawtooth structures promote osteoblastic differentiation of rat BMMSCs. Importantly, the large nano-sawtooth structure resulted in the greatest cell responses, including increased adhesion, proliferation, and differentiation. Conclusion: The enhanced adhesion, proliferation, and osteogenic differentiation abilities of rat BMMSCs on the nano-sawtooth structures suggest the potential to induce improvements in bonetitanium integration in vivo. Our study reveals the key role played by the nano-sawtooth structures on a titanium surface for the fate of rat BMMSCs and provides insights into the study of stem cellnanostructure relationships and the related design of improved biomedical implant surfaces.

show abstract

In vitro studies on the influence of surface modification of Ni–Ti alloy on human bone cells

Cited by 24 publications

References 72 publications

Biocompatible, Smooth, Plasma-Treated Nickel–Titanium Surface – An Adequate Platform for Cell Growth

Biocompatible, Smooth, Plasma-Treated Nickel–Titanium Surface – An Adequate Platform for Cell Growth

Silver incorporated antibacterial, cell compatible and bioactive titania layer on Ti metal for biomedical applications

Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells

Contact Info

Product

Resources

About