In VitroStimulation of Protein Synthesis in Uterine Microsomal Supernatant by Estrone Sulfate¹

Steroid sulfotransferases

“…17/3-Estradiol sulfonate, but not unconjugated 17/3-estradiol, is a potent inhibitor of glutathione S-transferase, a key enzyme in protection against metabolic stress (221). Estrone sulfonate directly produces ultrastructural changes (222) and has a specific effect on protein synthesis and secretion by endometrial glandular cells in primary culture (223,224). The activity of phospholipase A 2 in amniotic membranes is enhanced by the sulfonates of dehydroepiandrosterone and pregnenolone but not their unconjugated forms (225).…”

Metabolism of sodium oestrone [35S] sulphate in the rat

“…human, Butenandt & Hofstetter, 1939; the mare, Schachter & Marrian, 1938) suggests that it may represent an end product of metabolism in these species. However, Brooks, Leithauser, de Loecker & de Wever (1969) have claimed that sulphation of oestrone is a prerequisite of the hormone's stimulatory effect on protein synthesis in rat and rabbit uterine preparations.…”

Specific effect of oestrone sulphate on protein synthesis and secretion by cultured epithelial cells from guinea-pig endometrium

“…These modifications were distinguishable from those induced by oestradiol-17ß but resembled those observed under the influence of progesterone during the secretory phase of the endometrium. A direct effect of oestrone sulphate on protein synthesis in microsomal supernatants isolated from the uterus of both rat and rabbit has been reported by Brooks & Leithauser (1969). This effect was shown to be distinct from the action of oestradiol-17ß on induced proteins which is mediated through interactions with the oestradiol receptor.…”