<i>In vitro</i> stimulation of naive mouse lymphocytes by <i>Heligmosomoides polygyrus</i> adult worm antigens induces the production of IgG1

Robinson, Michael B.; Gustad, Thomas R.

doi:10.1046/j.1365-3024.1996.d01-52.x

Cited by 10 publications

(13 citation statements)

References 12 publications

(20 reference statements)

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“…For example, the nematode Heligmosomoides polygyrus contains immunomodulatory factors capable of either stimulating (1,2) or inhibiting (3,4) T and B cell responses. With respect to Ascaris suum, we and others have shown that infection with the nematode or the use of its soluble products or extracts at different stages of purification potentiates or suppresses the immune responses (5)(6)(7).…”

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confidence: 99%

Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum

Oshiro

Rafael

Enobe

et al. 2004

Braz J Med Biol Res

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The extract of Ascaris suum suppresses the humoral and cellular immune responses to unrelated antigens in the mouse. In order to further characterize the suppressive components of A. suum, we produced specific monoclonal antibodies which can provide an important tool for the identification of these proteins. The A. suum immunosuppressive fractions isolated by gel filtration from an extract of adult worms were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells and the cloned hybrid cells obtained were screened to determine the specificity of secreted antibodies. Three monoclonal antibodies named MAIP-1, MAIP-2 and MAIP-3 were selected and were shown to react with different epitopes of high molecular weight proteins from the A. suum extract.

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confidence: 99%

Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum

Oshiro

Rafael

Enobe

et al. 2004

Braz J Med Biol Res

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“…An extract of the intestinal nematode parasite Heligmosomoides polygyrus has recently been reported to stimulate the production of IgG1 in naive spleen cell culture in vitro (56). The data in that study showed that the extract mediated its effect by stimulating T cells to produce a factor (presumably IL-4) that induced IgG1 production in the culture.…”

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confidence: 94%

Extract ofNippostrongylus brasiliensisStimulates Polyclonal Type-2 Immunoglobulin Response by Inducing De Novo Class Switch

2000

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Infection with the nematode parasite Nippostrongylus brasiliensis induces a pronounced type-2 T-cell response that is associated with marked polyclonal immunoglobulin E (IgE) and IgG1 production in mice. To examine the differential roles of the infection and products produced by nematodes, we investigated a soluble extract of N. brasiliensis for the ability to mediate this type-2 response. We found that the extract induced a marked increase in IgE and IgG1 levels, similar to that induced by the infection. The extract did not affect the level of IgG2a in serum, showing that the effect was specific to IgE and IgG1 (type-2-associated immunoglobulin) rather than inducing a nonspecific increase in all immunoglobulin isotypes. This response was also associated with increased interleukin-4 production in vitro. These results confirm that the extract, like infection, is a strong

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“…It is important to note that both the immune response to nematodes and the modulation of that immune response by nematode factors are likely to be very complex. For example, Price and Turner (33) have shown that a somatic extract of Heligmosomoides polygyrus inhibits B-cell proliferation but, in contrast, Robinson and coworkers (38) reported that a similar extract stimulated IgG1 production in vitro. Moreover, extracts from A. suum have been reported to stimulate (20) and to inhibit (13, 39) B-cell function.…”

Section: Discussionmentioning

confidence: 99%

“…For example, in contrast to the immunostimulatory activity of the body fluid of Ascaris reported above, Ferreira and coworkers (13) and Soares and coworkers (39, 40) have reported immunomodulatory factors in secretions of A. suum, which ablate B-cell responses. Similarly, the nematode Heligmosomoides polygyrus has been reported to contain immunomodulatory factors capable of either stimulating (37,38) The prevailing evidence supports a significant role for accessory cells in the activation of T and B cells both in vivo and in vitro (7, 41). This has prompted numerous investigations into the role of macrophages in helminth-mediated immunomodulation of lymphocyte function (2,15,17,22,27,28,36).…”

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confidence: 99%

“…For example, in contrast to the immunostimulatory activity of the body fluid of Ascaris reported above, Ferreira and coworkers (13) and Soares and coworkers (39,40) have reported immunomodulatory factors in secretions of A. suum, which ablate B-cell responses. Similarly, the nematode Heligmosomoides polygyrus has been reported to contain immunomodulatory factors capable of either stimulating (37,38) or inhibiting (33,34) T-and B-cell responses. These studies demonstrate the diverse modulatory effects that nematodes or nematode factors have on developing immune responses.…”

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Modulation of B-Cell Proliferative Response by a Soluble Extract ofNippostrongylus brasiliensis

2000

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We and others have previously shown that nematodes or nematode products can stimulate or inhibit the generation of lymphocyte responses, suggesting that nematodes exert diverse effects on the developing immune responses of their host. In this study we examined the immunomodulatory effect of a soluble extract of Nippostrongylus brasiliensis (adult worm homogenate [AWH]) on B-cell responsiveness. We found that the extract inhibited the proliferation of B cells to lipopolysaccharide (LPS) stimulation in a dose-dependent manner. This effect was specific to B cells, since the extract did not inhibit T-cell proliferation to concanavalin A or anti-CD3 stimulation. The data presented here confirm that the extract is not toxic to B cells. We present evidence that the active factor is proteinaceous in nature and that the inhibitory activity is restricted to the adult stage of Nb. The extract does not appear to interfere with early activation events since it can be added up to 48 h after LPS stimulation, and it inhibited responses to phorbol myristate acetate and ionomycin. Furthermore, the proliferation of B cells to other activators was also inhibited by AWH. This observation shows that the inhibitory activity of AWH is not restricted to LPS-mediated B-cell proliferation. We present evidence that, in the absence of accessory cells, the inhibitory effect of the extract was ablated. This observation shows that the activity of AWH is not mediated directly on B cells but is mediated via the production of negative signals from accessory cells (macrophages), which affect a downstream pathway required by all B-cell activators tested. These effects on B-cell and accessory cell function are likely to have a significant effect on the outcome of infections experienced concurrently.To ensure survival and continuation of their life cycle, nematodes have evolved a unique relationship with their hosts. Part of this accommodation is the ability to modulate host immune responsiveness (25). Modulation of lymphocyte function has been widely reported among nematodes (2,3,19,21,22,28) and other parasites (6,15,27,36). For example, we have previously shown a dramatic potentiation of antibody responses to third party antigens by treatment with a body fluid extract of Ascaris suum (19). This observation is significant in that it suggests that the modulatory effect of nematodes on immune responses is far-reaching and may have profound effects on developing immune responses to unrelated antigenic challenge. We have also reported the presence of a B cell mitogen in the body fluid of A. suum, which stimulated G 0 B cells to enter the cell cycle (20). This activity of the Ascaris factor was dependent on viable accessory cells.The effect of helminth products on the developing immune response is likely to be complex. For example, in contrast to the immunostimulatory activity of the body fluid of Ascaris reported above, Ferreira and coworkers (13) and Soares and coworkers (39, 40) have reported immunomodulatory factors in secretions of A. suum, which a...

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In vitro stimulation of naive mouse lymphocytes by Heligmosomoides polygyrus adult worm antigens induces the production of IgG1

Cited by 10 publications

References 12 publications

Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum

Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum

Extract ofNippostrongylus brasiliensisStimulates Polyclonal Type-2 Immunoglobulin Response by Inducing De Novo Class Switch

Modulation of B-Cell Proliferative Response by a Soluble Extract ofNippostrongylus brasiliensis

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