In VitroSkin Permeation Methodology

Finnin, Barrie C.; Walters, K.; Franz, Thomas

doi:10.1002/9781118140505.ch5

Cited by 25 publications

(22 citation statements)

References 88 publications

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“…The phosphate buffer solution was chosen for maintenance of the physiological conditions. 37 1%SLS was added to maintain sink conditions. The rotation was adjusted at 50 rpm and the temperature of the medium was kept at 37±0.5 °C.…”

Section: Eementioning

confidence: 99%

Formulation of Nanospanlastics as a Promising Approach for ‎Improving the Topical Delivery of a Natural Leukotriene Inhibitor (3-‎Acetyl-11-Keto-β-Boswellic Acid): Statistical Optimization, in vitro ‎Characterization, and ex vivo Permeation Study

Badria¹,

Mazyed²

2020

DDDT

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The current study aimed to discuss the potential of nanospanlastics as a surfactantbased vesicular system for improving the topical delivery of 3-acetyl-11-keto-β-boswellic acid (AKBA). AKBA is a potent anti-inflammatory drug, but it has poor oral bioavailability due to its poor aqueous solubility. Moreover, the topical delivery of AKBA is difficult due to its high lipophilicity. To overcome these drawbacks, AKBA was formulated as deformable elastic nanovesicles and nanospanlastics, for improving its topical delivery. Materials and Methods: AKBA-loaded spanlastic nanovesicles (SNVs) were formulated by ethanol injection technique according to 2 3 factorial design using Span 60 as a non-ionic surfactant and Tween 80 as edge activator (EA) to investigate the effect of different independent variables on entrapment efficiency (EE%), % drug released after 8 hr (Q 8h) and particle size (PS) using Design-Expert software. In vitro characterization, stability test and ex vivo permeation study of the optimized formula were performed. Results: The choice of the optimized formula was based on the desirability criteria. F7 was selected as the optimized formula because it has the highest desirability value of 0.648. F7 exhibited EE% of 90.04±0.58%, Q 8h of 96.87±2.67%, PS of 255.8±2.67 nm, and zeta potential of −49.56 mV. F7 appeared as spherical well-defined vesicles in both scanning electron microscope (SEM) and transmission electron microscope (TEM). The Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) studies investigated the absence of interaction between AKBA and different excipients and good encapsulation of AKBA within SNVs. F7 retained both physical and chemical stability after storage for 3 months at 4-8 °C. Ex vivo permeation test exhibited significant enhancement of permeability of F7 across rat skin than the free drug. Conclusion: Nanospanlastics could be a promising approach for improving the permeability and topical delivery of AKBA.

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Section: Eementioning

confidence: 99%

Formulation of Nanospanlastics as a Promising Approach for ‎Improving the Topical Delivery of a Natural Leukotriene Inhibitor (3-‎Acetyl-11-Keto-β-Boswellic Acid): Statistical Optimization, in vitro ‎Characterization, and ex vivo Permeation Study

Badria¹,

Mazyed²

2020

DDDT

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“…Considerable individual variation in the absorption of methimazole was observed between the 6 cats (Figs & ). High intra‐ and interindividual variability is expected in in vitro transdermal trials and reflects the variability in the cat population (Finnin et al ., ). Individual variation between cats was also seen in a previous, in vitro study of methimazole in two transdermal formulations.…”

mentioning

confidence: 97%

Regional variations in percutaneous absorption of methimazole: an in vitro study on cat skin

Hill

Chambers

Jones

et al. 2015

Vet Pharm & Therapeutics

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The use of transdermal gel medications in cats has become popular in veterinary medicine due to the ease of administration compared to oral medication. The research to support systemic absorption of drugs after transdermal gel administration and the preferred skin region to apply these drugs in cats is limited. The aim of this study was to characterize the effect of different skin regions on the percutaneous absorption pharmacokinetics of a commercially available transdermal methimazole after a finite dose was applied to feline skin in vitro. A commercial formulation of methimazole (10 mg) was applied to four skin regions (the inner stratum corneum of the ear, groin, neck, and thorax regions) from six cats. The receptor medium was sampled up to 36 h postapplication, and methimazole concentrations were measured using high-performance liquid chromatography. Methimazole was absorbed more completely across the pinnal skin, compared to the groin, neck, and thorax (P < 0.001), which justifies application to the pinna to maximize efficacy and also to minimize the effects of grooming.

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“…High intra- and inter-individual variability is expected in in vitro transdermal trials. 29 In the current study, each cat received the same dose to the right and left ear, and the left and right ears were compared to determine inter-individual differences. A difference in methimazole absorption was found between the left and right ears, and there was also considerable variability between subjects for the methimazole concentrations remaining in the ear (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

Trans-pinnal movement of methimazole: an in vitro study showing that methimazole can cross from the inner to outer pinna of cats

Hill

Chambers

Jones

et al. 2015

Journal of Feline Medicine and Surgery

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In VitroSkin Permeation Methodology

Cited by 25 publications

References 88 publications

<p>Formulation of Nanospanlastics as a Promising Approach for ‎Improving the Topical Delivery of a Natural Leukotriene Inhibitor (3-‎Acetyl-11-Keto-β-Boswellic Acid): Statistical Optimization, in vitro ‎Characterization, and ex vivo Permeation Study</p>

<p>Formulation of Nanospanlastics as a Promising Approach for ‎Improving the Topical Delivery of a Natural Leukotriene Inhibitor (3-‎Acetyl-11-Keto-β-Boswellic Acid): Statistical Optimization, in vitro ‎Characterization, and ex vivo Permeation Study</p>

Regional variations in percutaneous absorption of methimazole: an in vitro study on cat skin

Trans-pinnal movement of methimazole: an in vitro study showing that methimazole can cross from the inner to outer pinna of cats

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