<i>In Vitro</i>Simulation of Corneal Epithelium Microenvironment Induces a Corneal Epithelial-like Cell Phenotype from Human Adipose Tissue Mesenchymal Stem Cells

Nieto-Miguel, Teresa; Galindo, Sara; Reinoso, Roberto; Corell, Alfredo; Martino, Mario; Pérez-Simón, Josè Antonio; Calonge, Margarita

doi:10.3109/02713683.2013.802809

Cited by 75 publications

(59 citation statements)

References 46 publications

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“…Several therapeutic approaches with alternative stem cells, such as mesenchymal stem cells (MSC) [12–16], cultured oral mucosa epithelial cells [17, 18], embryonic stem cells (ESC) [19], or induced pluripotent stem cells (IPSC) [20–22], have been established to either study their potential to differentiate into corneal epithelium phenotypes or to reconstruct a damaged corneal epithelium in experimental models. However, several key issues remain unresolved and these options require a great deal of development before they are ready for clinical application in humans.…”

Section: Introductionmentioning

confidence: 99%

Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application

Nieto‐Nicolau

Martínez-Conesa

Casaroli‐Marano

2016

Stem Cells International

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Limbal stem cells (LSC) are the progenitor cells that maintain the transparency of the cornea. Limbal stem cell deficiency (LSCD) leads to corneal opacity, inflammation, scarring, and blindness. A clinical approach to treat this condition consists in LSC transplantation (LSCT) after ex vivo expansion of LSC. In unilateral LSCD, an autologous transplant is possible, but cases of bilateral LSCD require allogenic LSCT. Cadaveric donors represent the most important source of LSC allografts for treatment of bilateral LSCD when living relative donors are not available. To evaluate the suitability of aged cadaveric donors for LSCT, we compared three pools of LSC from donors of different ages (<60 years, 60–75 years, and >75 years). We evaluated graft quality in terms of percent of p63-positive (p63+) cells by immunofluorescence, colony forming efficiency, and mRNA and protein expression of p63, PAX6, Wnt7a, E-cadherin, and cytokeratin (CK) 12, CK3, and CK19. The results showed that LSC cultures from aged donors can express ≥3% of p63+ cells—considered as the minimum value for predicting favorable clinical outcomes after LSCT—suggesting that these cells could be a suitable source of LSC for transplantation. Our results also indicate the need to evaluate LSC graft quality criteria for each donor.

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Section: Introductionmentioning

confidence: 99%

Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application

Nieto‐Nicolau

Martínez-Conesa

Casaroli‐Marano

2016

Stem Cells International

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“…Inducing MSCs in the AM significantly improves the reconstruction of the corneal surface of rats with corneal alkali burn. In a recent in vitro experiment, corneal epithelia-like cells were induced from human adipose tissue-derived MSCs by subjecting them to a medium conditioned with corneal epithelial cells [53] . In addition, MSCs can differentiate into corneal epithelial progenitor cells in Rb-LSC deficiency models [54] .…”

Section: Msc Mobilization and Homingmentioning

confidence: 99%

Mesenchymal stem cells: Potential role in corneal wound repair and transplantation

Li¹

2014

WJSC

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Corneal diseases are a major cause of blindness in the world. Although great progress has been achieved in the treatment of corneal diseases, wound healing after severe corneal damage and immunosuppressive therapy after corneal transplantation remain problematic. Mesenchymal stem cells (MSCs) derived from bone marrow or other adult tissues can differentiate into various types of mesenchymal lineages, such as osteocytes, adipocytes, and chondrocytes, both in vivo and in vitro . These cells can further differentiate into specific cell types under specific conditions. MSCs migrate to injury sites and promote wound healing by secreting anti-inflammatory and growth factors. In addition, MSCs interact with innate and acquired immune cells and modulate the immune response through their powerful paracrine function. Over the last decade, MSCs have drawn considerable attention because of their beneficial properties and promising therapeutic prospective. Furthermore, MSCs have been applied to various studies related to wound healing, autoimmune diseases, and organ transplantation. This review discusses the potential functions of MSCs in protecting corneal tissue and their possible mechanisms in corneal wound healing and corneal transplantation.

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“…Optical PK following the COMET treatment has been reported by a group to achieve good improvement in the visual outcome with a stable corneal graft (Ma et al 2009 ). Recent studies have also shown that mesenchymal stem cells derived from various sources such as bone marrow, dental pulp, adipose tissues and limbal stroma are effective in controlling infl ammation and regeneration of corneal surface (Gu et al 2009 ;Gomes et al 2010 ;Nieto-Miguel et al 2013 ;Rohaina et al 2014 ;Katikireddy et al 2014 ;Acar et al 2015 ;Syed-Picard et al 2015 ). Another group has reported the presence of stromal stem cells at the limbal niche and demonstrated the usefulness of these autologous, mesenchymal-like stem cells in the treatment of corneal stromal scars (Li et al 2012 ;Basu et al 2014 ).…”

Section: Alternate Stem Cell Sources and Treatment Modalities For Ocumentioning

confidence: 99%

Regenerative Therapies for the Ocular Surface

Vemuganti¹,

Sangwan²,

Mariappan³

et al. 2016

Regenerative Medicine - From Protocol to Patient

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Integrity of ocular surface depends on adequate tear fi lm and stability of the surface epithelium consisting of two specialized phenotypically different epithelial cells, the central transparent corneal epithelium and the peripheral conjunctival cells, separated by a more specialized transition zone, called the limbus. Similar to the epithelial regeneration in other parts of the body, the corneal epithelium is regenerated from the stem cells located in limbus. Severe chemical burns and other diseases can cause damage to the limbus, resulting in a condition called Limbal Stem Cell Defi ciency (LSCD). Effective therapeutic modalities for this visionthreatening condition include use of human amniotic membrane, replenishing the stores of limbal stem cells by limbal transplantation. However last decade has witnessed the use of ex-vivo expanded sheet of limbal epithelial cells for ocular surface reconstruction in such cases. Our group has established a simple, feeder-cell free, cost-effective way of culturing the corneal epithelium from limbal tissues within 2 weeks, using human amniotic membrane as a vehicle. The interim results of a clinical trial involving 700 patients with severe unilateral and bilateral LSCD revealed

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In VitroSimulation of Corneal Epithelium Microenvironment Induces a Corneal Epithelial-like Cell Phenotype from Human Adipose Tissue Mesenchymal Stem Cells

Cited by 75 publications

References 46 publications

Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application

Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application

Mesenchymal stem cells: Potential role in corneal wound repair and transplantation

Regenerative Therapies for the Ocular Surface

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