<i>In vitro</i> Screening of Antioxidant, Anti-Inflammatory activity of <i>Vitex negundo</i> Methanolic leaf extract

Anusha, D; Kancherla, Nagaraju; Chellathai, Darling

doi:10.5958/0974-360x.2019.00475.x

Cited by 4 publications

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“…Plant-based antioxidant compounds play a defensive role by preventing the generation of free radicals and hence are extremely beneficial to alleviating the diseases caused by oxidative stress. 2 A number of medicinal plants with potent antioxidant and anti-inflammatory potential are Artemisia vulgaris, 3 Vitex negundo, 4 Albizia myriophylla, 5 Cressa cretica 6 . Mitragyna parvifolia is also an important tree with antioxidant properties 7 and thus could be a potential candidate for treatments in inflammation.…”

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confidence: 99%

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2022

IJPSR

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Inflammation is considered a primary physiologic defense mechanism that helps the body to protect itself against infection, burn, toxic chemicals, allergens, or other noxious stimuli. Inflammatory diseases are currently treated with steroidal and non-steroidal anti-inflammatory drugs (NSAIDs). Unfortunately, both of these widely prescribed drug classes have significant negative side effects. Hence, there is a need to develop new drugs with novel modes of action that do not produce considerable side effects. The use of traditional medicine and medicinal plants in most developing countries as a normative basis for the maintenance of good health, has been widely observed. So, crude flavonoid fraction of Mitragyna parvifolia was screened for antiinflammatory activity using Carrageenan induced method and Formalin induced method at various doses (50,100 &200 mg/kg), and the results were compared with standard drug diclofenac sodium (20 mg/kg). It showed significant activity at 200 mg/kg. The Crude flavonoid fraction was also screened for antioxidant activity at various concentrations (20, 40, 60, 80,100μg/ml) using the DPPH free radical scavenging method. The antioxidant activity was concentrationdependent which was compared with standard antioxidant ascorbic acid. The highest antioxidant activity of CFF of M. parvifolia was observed at a concentration of 100μg/ml. INTRODUCTION:Reactive oxygen species (ROS) such as superoxide anion (O 2 •-), hydroxyl radicals (OH • ), and hydrogen peroxide (H 2 O 2 ) play a major role in the development of oxidative stress that can lead to many illnesses, including cardiovascular diseases, diabetes, inflammation, psychiatric diseases (depression, epilepsy, dementia and anxiety), cancer, anaemia, ischemia etc. 1 . Exposure of skin to solar radiation causes harmful effects like sunburn, premature ageing of skin and skin cancers.

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confidence: 99%

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2022

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Chemical Datasets, Antioxidant, Free Radicals Scavenger activities estimate in Aqueous Garlic (Allium sativum) extract

Jasim¹,

Al-Hilu²

2021

RJPT

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Garlic is one of many medicinal plants that used over thousands of years due to its organosulfur compounds. Materials and methods: commercial garlic was used for the extraction, phytochemicals detections and Gas Chromatography-Mass (GC- Mass) analysis utilized to determine the main compounds in the aqueous extract. Antioxidant activity was evaluated in the garlic extract using total antioxidant capacity (TAC) and DPPH for detecting the concentration that takes half of the free radicals. The phytochemical tests revealed several compounds in the extract, included alkaloid, glycosides, tannins, and flavonoid and phenol while saponin compound was not detected. GC-Mass spotted 15 different compounds included allyl trisulfide with the concentration (1.89%) followed by ascorbic acid 2, 6-dihexadecanoate (22.24%), 9, 12-Octadecadienoic acid (Z, Z) (11.44), and 6-Octadecenoic acid, (Z) (40, 28). Total Antioxidant Activity of garlic aqueous extract reached 60 mg/ml compared to that of ascorbic acid. IC50 examination observed that the aqueous extract of garlic had 308.57µg/ml value.

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In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F.

Krishnaveni

Samuel

2021

RJPT

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Clerodendrum phlomid is was a common shrub of arid plains, low hills, and tropical deserts. It belongs to the family Verbenaceae. Many species of this genus have been documented in traditional system of medicine in India. The roots of the plant have been extensively studied in Ayurveda but in folk and traditional medicine, it is the leaf that finds more application in alleviating many diseases. Oxidation byproducts of metabolism was responsible for many pathophysiological conditions. Anti-oxidants are molecules that quench the free radicals. The present study aims at evaluating the in-vitro anti-oxidant and anti-inflammatory activity of the aqueous and ethanol leaf extract of Clerodendrum phlomidis. The anti-oxidant activity was evaluated by three in vitro models, namely the DPPH radical scavenging activity, hydroxyl radical scavenging activity and NO radical scavenging activity. The anti-inflammatory activity was evaluated by the RBC membrane Inhibition method. The aqueous extract was found to have better anti- oxidant activity than the ethanol extract in DPPH and NO radical scavenging activity. The RBC membrane inhibition method clearly states that the aqueous leaf extract of Clerodendrum phlomidis was found to have a comparatively an effective anti-inflammatory activity than ethanol extract.

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In vitro Screening of Antioxidant, Anti-Inflammatory activity of Vitex negundo Methanolic leaf extract

Cited by 4 publications

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Chemical Datasets, Antioxidant, Free Radicals Scavenger activities estimate in Aqueous Garlic (Allium sativum) extract

In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F.

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