1997
DOI: 10.1073/pnas.94.14.7667
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In vitro reconstitution of the photosystem I light-harvesting complex LHCI-730: Heterodimerization is required for antenna pigment organization

Abstract: Here we describe the in vitro reconstitution of photosystem I light-harvesting complexes with pigments and proteins (Lhca1 and Lhca4) obtained by overexpression of tomato Lhca genes in Escherichia coli. Using Lhca1 and Lhca4 individually for reconstitution results in monomeric pigmentproteins, whereas a combination thereof yields a dimeric complex. Interactions of the apoproteins is highly specific, as reconstitution of either of the two constituent proteins in combination with a light-harvesting protein of ph… Show more

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Cited by 155 publications
(215 citation statements)
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“…The close relation between Lhca2 and Lhca4 is confirmed by sequence analysis, which shows 55% identity and 75% homology (14). Surprisingly, the distribution of red forms does not fit into the picture, because these are associated mainly with Lhca3 and Lhca4 (fluorescence emission at 725-730 nm), whereas Lhca1 and Lhca2 have emission at higher energy (702 nm) (12,13,15). Based on high homology between proteins with different content in red forms, a sequence motif associated with the presence of lowest absorption band, common to Lhca3 and Lhca4 and not present in Lhca1 and Lhca2, should possibly be detected.…”
mentioning
confidence: 68%
“…The close relation between Lhca2 and Lhca4 is confirmed by sequence analysis, which shows 55% identity and 75% homology (14). Surprisingly, the distribution of red forms does not fit into the picture, because these are associated mainly with Lhca3 and Lhca4 (fluorescence emission at 725-730 nm), whereas Lhca1 and Lhca2 have emission at higher energy (702 nm) (12,13,15). Based on high homology between proteins with different content in red forms, a sequence motif associated with the presence of lowest absorption band, common to Lhca3 and Lhca4 and not present in Lhca1 and Lhca2, should possibly be detected.…”
mentioning
confidence: 68%
“…The low-energy emission of the Lhca1/4 dimer is known to originate from the red Chls of the Lhca4 monomeric subunit (30). The role of Lhca1 in the observed spectral dynamics of Lhca1/4, and whether similar dynamics are exhibited when Lhca1 is absent, was of interest.…”
Section: Resultsmentioning
confidence: 99%
“…The degree of mixing and accordingly the emission maximum strongly depend on the local protein environment and in particular on the nature of the ligand of Chl 603 (22). Lhca1 and Lhca2, which contain the ligand histidine (H), have low-temperature emission maxima at 690 and 701 nm, respectively, whereas the ligand asparagine (N) in Lhca3 and Lhca4 assists in shifting the maxima to 725 and 732 nm, respectively (29,30). Changing in Lhca4 the ligand of Chl 603 from an N to an H, thus producing the mutant Lhca4-NH, shifts the low-temperature emission maximum to 686 nm, while an additional emission band is present at approximately 702 nm (22).…”
mentioning
confidence: 99%
“…Lhca1 seems to possess the less red-shifted spectral forms (684 nm) (14,15), which is in agreement with its close relatedness to a minor lightharvesting polypeptide of PSII, CP29. On the contrary, the Lhca4 binds the "reddest" pigments (9,16).…”
mentioning
confidence: 99%