In vitropropagation ofInula verbascifolia(Willd.) Hausskn. subsp.verbascifolia

Perica, Mirna Ćurković; Vršek, Ines; Mitić, Božena

doi:10.1080/11263500701872119

Cited by 10 publications

(7 citation statements)

References 10 publications

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“…For root induction, MS basal medium with 1.0 mg/l IAA was found superior. Higher efficacy of IAA over other auxins for root induction has been demonstrated in many medicinal plants of Asteraceae family, like Stevia rebaudiana (Ahmed et al 2007), Inula verbascifolia (Perica et al 2008), Artemisia petrosa (Pace et al 2004). …”

Section: Resultsmentioning

confidence: 99%

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Nayak

Kalidass

2016

Plant Tissue Cult. & Biotech.

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Multiple shoots were induced on cotyledon explants of in vitro grown seedlings of Blepharispermum subsessile DC, cultured on MS medium supplemented with various combinations and concentrations of BAP, IBA and GA 3 . The highest regenerative response was observed on medium containing 2.5 mg/l BAP where shoot buds initiated after 12 days of inoculation and about 32 shoots were produced in 30 days time. Addition of GA 3 played a key role in leaf expansion and elongation of shoot buds. Addition of the auxin IBA to the induction medium resulted in more callus proliferation rather than shoot bud induction. The elongated shoots were transferred to root induction medium consisting of half strength MS supplemented with IAA, NAA and IBA. Highest rooting response (90%) was recorded in ½ MS supplemented with 1.0 mg/l IAA. Acclimatized plants were maintained in polybags with garden soil for future reintroduction program to their natural habitat.

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Section: Resultsmentioning

confidence: 99%

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Nayak

Kalidass

2016

Plant Tissue Cult. & Biotech.

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“…Hausskn. Perica et al (2008) recorded a highest multiplication rate of 6.5 shoots/node explant on MS supplemented with 2.2 mM/L BAP and 2.9 mM/L GA3 (Gibberellic acid) and Thiem et al (2003) induced indirect adventitious shoots on MS containing 0.88 μg/L BAP. Trejgell et al (2018) obtained, on MS medium containing 1.0 mg/L BAP and 0.1 mg/L NAA, the highest caulogenesis rate (83.3%) on hypocotyl explant of I. germanica L., and the highest average number of shoots per shoot tip explant (12.0).…”

Section: Resultsmentioning

confidence: 97%

Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+

BOKRI

KAHLAOUI

Hcini

et al. 2023

Not Bot Horti Agrobo

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The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal and horticultural interests. The objective was to identify the culture media that support the best organogenesis and callogenic expressions of four types of aerial explants; nodal and internodal segments and leaf portions, by varying the mineral composition of twenty culture media by adding increasing concentrations of Na+ (from 0-100 mM) and K+ (from 0-50 mM). After two months of culture, parameters relating to the various expressions of organogenesis and callogenesis were measured. Results showed that the K+ and Na+ interactions affect the explants development. The combination of high concentrations of Na+ (50, 100 mM) and K+ (50 mM) ions allowed the most important regeneration of the axillary shoots (70.6-100%), root neoformation (82.6-96.1%) and callus induction (76.3-100%). Rooted plantlets with well-developed axillary shoots have been successfully acclimatized with a 93% of survival rate. The selected media would allow a large-scale multiplication of this medicinal species, without adding exogenous phytohormones, and could be used for the micropropagation of other threatened halophyte species or for the production of callus; rich in secondary metabolites.

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“…Data regarding the micropropagation of Inula species is scarce, with only five studies involving four species, namely, I. racemosa , I. verbascifolia subsp. verbascifolia , I. germanica , and I. royleana [ 20 , 21 , 22 , 23 ]. Most similar to our work was the regeneration protocol developed for I. racemosa nodal segments obtained from in vitro germinated seeds and cultivated in MS medium supplemented with 0.25 mg/L BAP alone that had the maximum number of shoots (20.7 shoots/explants) [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

A First Approach for the Micropropagation of the Edible and Medicinal Halophyte Inula crithmoides L.

Rodrigues

Castañeda-Loaiza

Fernandes

et al. 2023

Plants

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Inula crithmoides L. (golden samphire) is an edible aromatic halophyte species with confirmed nutritional and medicinal properties attributed to the presence of important metabolites, including proteins, carotenoids, vitamins, and minerals. Therefore, this study aimed at establishing a micropropagation protocol for golden samphire that can serve as a nursery approach to its standardized commercial cultivation. For that purpose, a complete regeneration protocol was developed by improving shoot multiplication from nodal explants, rooting, and acclimatization methodologies. The treatment with BAP alone induced the maximum shoot formation (7–7.8 shoots/explant), while IAA treatment increased the shoot height (9.26–9.5 cm). Furthermore, the treatment that coupled best shoot multiplication (7.8 shoots/explant) and highest shoot height (7.58 cm) was MS medium supplemented with 0.25 mg/L BAP. Moreover, all shoots produced roots (100% rooting), and multiplication treatments did not exert significant effect on root length (7.8–9.7 cm/plantlet). Moreover, by the end of the rooting phase, plantlets cultivated with 0.25 mg/L BAP had the highest shoot number (4.2 shoots/plantlet), and plantlets from 0.6 mg/L IAA + 1 mg/L BAP presented the highest shoot height (14.2 cm) similar to control plantlets (14.0 cm). The survival up to the ex-vitro acclimatization stage was increased from 9.8% (control) to 83.3%, when plants were treated with a paraffin solution. Nevertheless, the in vitro multiplication of golden samphire is a promising way for its rapid propagation and can be used as a nursery method, contributing to the development of this species as an alternative food and medicinal crop.

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In vitropropagation ofInula verbascifolia(Willd.) Hausskn. subsp.verbascifolia

Cited by 10 publications

References 10 publications

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+

A First Approach for the Micropropagation of the Edible and Medicinal Halophyte Inula crithmoides L.

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