<i>In vitro</i>pollen germination of some wild species of pigeonpea (<i>Cajanus cajan</i>) using PGM droplet technique

Jayaprakash, P.; Sabesan, T.

doi:10.5958/j.0975-6906.73.2.031

Cited by 7 publications

(3 citation statements)

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“…• PGM droplet technique [15]: In a Petri dish a droplet of pollen germination medium was placed using a glass rod. Thus drops of different media could be placed within few centimeter distance between them For example nine media can be placed in 3 × 3 fashion (Figure 2).…”

Section: Pollen Germination Methodsmentioning

confidence: 99%

Pollen Germination in vitro

Jayaprakash¹

2018

Pollination in Plants

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Pollen germination in vitro is a reliable method to test the pollen viability. It also addresses many basic questions in sexual reproduction and particularly useful in wide hybridization. Many pollen germination medium ranging from simple sugars to complex one having vitamins, growth regulators, etc. in addition to various minerals have been standardized to germinate pollen artificially. The different media, successful pollen germination methods, procedures from pollen germination studies with wheat, rye, brinjal, pigeonpea and its wild relatives are discussed.

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Section: Pollen Germination Methodsmentioning

confidence: 99%

Pollen Germination in vitro

Jayaprakash¹

2018

Pollination in Plants

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“…Pollen was collected from appropriate ear heads by cutting off half the spikelet. Once anther started dehiscence, the pollen was dusted directly on to the germination medium by gentle tapping and cultured according to the pollen germination media (PGM) droplet method (Jayaprakash et al 2013). All observations on pollen germination and pollen tube growth were recorded 15 min after incubation at 17±1°C for wheat pollen.…”

Section: Pollen Collection and Culturementioning

confidence: 99%

“…However, even 13% PEG concentration showed pollen tube bursting. The role of PEG in pollen germination medium was well established and choosing appropriate molecular weight is the key for specific crop (Jayaprakash and Sabesan 2013. ).…”

Section: Polyethylene Glycol 6000 Supplemented Mediummentioning

confidence: 99%

An improvedin vitrogermination medium for recalcitrant bread wheat (Triticum aestivumL.) pollen

Jayaprakash¹,

Annapoorani²,

Vikas³

et al. 2015

Intern. Jour. Contemp. Microbiol.

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There is no robust and quick technique available for testing germination of wheat pollen. In this study, Brewbaker and Kwack (BK) pollen germination medium (PGM) was adjusted with α α α α α-amino caproic acid (EACA), peptone water and maltose to develop an improved medium for wheat. Germination of pollen in this PGM ranged from 92% to 98.2% depending upon genotype pollen germination media (PGM). This is the first report of PGM which has >95% wheat pollen germination in vitro.

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