<i>In vitro</i> phototoxicity test using artificial skin with melanocytes

Lee, Jong Hee; Kim, Ji Eun; Kim, Beom Joon; Cho, Kwang Hyun

doi:10.1111/j.1600-0781.2007.00279.x

Cited by 15 publications

(12 citation statements)

References 19 publications

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“…Melanocytes, although normally present in fresh keratinocyte cultures and dermo-epidermal constructs (Rehder et al 2004) were specifically investigated in the work of Hedley and co-workers (Hedley et al 2002) where the regulatory role for fibroblasts in skin pigmentation was revealed since, when added to in vitro skin constructs, fibroblasts downregulated spontaneous pigmentation. Melanocyte-containing skin constructs are used extensively for in vitro studies of ultraviolet light effects on the skin such as phototoxicity and photoageing (Marrot et al 1998;Lee et al 2007).…”

Section: Tissuetech Autograft Systemmentioning

confidence: 99%

A review of tissue-engineered skin bioconstructs available for skin reconstruction

Shevchenko

James

2009

J. R. Soc. Interface.

615

469

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Situations where normal autografts cannot be used to replace damaged skin often lead to a greater risk of mortality, prolonged hospital stay and increased expenditure for the National Health Service. There is a substantial need for tissue-engineered skin bioconstructs and research is active in this field. Significant progress has been made over the years in the development and clinical use of bioengineered components of the various skin layers. Off-the-shelf availability of such constructs, or production of sufficient quantities of biological materials to aid rapid wound closure, are often the only means to help patients with major skin loss. The aim of this review is to describe those materials already commercially available for clinical use as well as to give a short insight to those under development. It seeks to provide skin scientists/tissue engineers with the information required to not only develop in vitro models of skin, but to move closer to achieving the ultimate goal of an off-the-shelf, complete full-thickness skin replacement.

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Section: Tissuetech Autograft Systemmentioning

confidence: 99%

A review of tissue-engineered skin bioconstructs available for skin reconstruction

Shevchenko

James

2009

J. R. Soc. Interface.

615

469

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“…At present, it has been successfully conducted in vitro, but no clinical validation is reported 10,11) . At the same time, preparation of functional dermal substitute containing epidermis, FB, VEC, Langhan's cell, sweat gland cell, hair follicle cell and pigment cell, and constructing ideal tissue-engineered dermal substitute have also be attempted 12,13) . Application of dermal substitute could effectively improve the utilization rate of limited autologous skin source 5) , reconstruct the dermal tissue structure, and significantly reduce the postoperative scar hyperplasia and contracture deformity.…”

Section: Discussionmentioning

confidence: 99%

Vascularization of Novel Porcine Acellular Dermal Matrix

Bian

Chen

et al. 2014

J. Hard Tissue Biology.

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The aims of this study were to observe the growth of vascular endothelial cells (VECs) and the vascularization of novel porcine acellular dermal matrix (PADM), and to investigate the methods for modifying heterologous dermal substitutes in order to improve their compatibility. Twenty-four Wistar rats were divided into a PADM group and a HADM group, which were transplanted with PADM and HADM, respectively. At the 3rd day, and at the 1st, 2nd, and 4th week after the operation, the dermal substitute was removed, and HE staining and CD34 and factor VIII immunohistochemical staining were performed. The distribution and proliferation of VEC, the vascularization, and histological changes in the dermal substitute were observed. At the 3rd postoperative day, tiny capillaries sprouted in the dermal substitute and began to proliferate. At the 1st postoperative week, the new capillaries extended into the dermal substitute. At the 2nd postoperative week, the new capillaries had entered the dermal substitute and further increased in number, and were more mature than the capillaries in the 1 st postoperative week (P<0.05). At the 4th postoperative week, the capillaries were distributed everywhere in the dermal substitute, along with further matured VECs, but the capillary number was not significantly increased compared with the 2nd postoperative week (P> 0.05). There was a good VEC proliferation in implanted PADM, with immigrated fibroblasts and matured capillaries. The novel PADM showed good tissue compatibility.

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“…Other potential UVA/UVB biomarkers include: inflammation markers such as interleukins and their receptors (22), protein oxidation markers (23), or other noninvasive markers such as skin autofluorescence changes (24). In a typical protocol, 10-20 subjects (age 18-60 years; Fitzpatrick skin types II and III) will be randomized and treated with antioxidant product or vehicle control on the lower back for four consecutive days (1).…”

Section: In Vivo Testing Of Antioxidants In Humans Uv Induction Modelmentioning

confidence: 99%

“…In general, biomarkers indicate changes in sunburn cell formation, thymine dimer formation, matrix metalloproteinase-9 expression, and p53 protein expression. Other potential UVA/UVB biomarkers include: inflammation markers such as interleukins and their receptors (22), protein oxidation markers (23), or other noninvasive markers such as skin autofluorescence changes (24). Effects of UV irradiation on immunosuppression can also be quantified by evaluating specific Langerhans cells markers.…”

Section: In Vivo Testing Of Antioxidants In Humans Uv Induction Modelmentioning

confidence: 99%

Antioxidants and the skin: Understanding formulation and efficacy

et al. 2012

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Antioxidants are molecules capable of inhibiting the oxidation of other molecules. Although oxidation reactions are essential for life, they can also be damaging. All living organisms maintain complex systems of multiple types of antioxidants to protect their cells from oxidative damage. Antioxidants can also act as pro-oxidants, under certain circumstances. The efficacy and benefit of an antioxidant is, therefore, very much dependent on the delivery of the antioxidant to the organism. Topically applied antioxidants constitute an important group of pharmacologically active agents capable of preventing the occurrence and reducing the severity of UV-induced skin damage and skin aging. Antioxidants protect skin cells against the damaging effects of reactive oxygen species (ROS), such as singlet oxygen, superoxide, peroxyl radicals, hydroxyl radicals, and peroxynitrite. ROS induced oxidative stress in the skin has been linked to cancer, aging, inflammation, and photodamage. This review focuses on antioxidants used in the cosmetic industry for protection of skin, formulation methods used to enhance their efficacy, and methods used to test the efficacy of antioxidants in topical formulations.

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In vitro phototoxicity test using artificial skin with melanocytes

Abstract: The AS with melanocytes may be a useful tool especially for examining UV-induced cutaneous changes and a promising in vitro phototoxicity test model for topical agents.

Cited by 15 publications

References 19 publications

A review of tissue-engineered skin bioconstructs available for skin reconstruction

A review of tissue-engineered skin bioconstructs available for skin reconstruction

Vascularization of Novel Porcine Acellular Dermal Matrix

Antioxidants and the skin: Understanding formulation and efficacy

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