In Vitro morphological, optical and microbiological evaluation of nanosilver fluoride in the remineralization of deciduous teeth enamel

Abstract

BackgroundNanosilver fluoride (NSF) was developed as an alternative in the prevention of dental caries.PurposeThe aim of this study was to test the remineralizing action of NSF on incipient enamel caries and its antimicrobial action on the acid production and adhesion of Streptococcus mutans.MethodsDeciduous enamel fragments were treated with sodium fluoride (NaF), NSF and deionized water. Microhardness, fluorescence spectroscopy and optical coherence tomography imaging were performed on each specimen before chemical caries induction, after caries induction and after 14 days of pH cycling. The treated enamel fragments were also placed into test tubes containing bacterial suspension and saliva. The pH readings and quantification of the adhered microorganisms to the dental enamel were determined. Analysis of variance, Kruskal-Wallis, Mann-Whitney, Tukey and mixed linear regression model were applied.ResultsNSF and NaF were effective in enamel remineralization, with a statistically significant difference (p<0.001) to deionized water, and they had no statistically significant difference between themselves (p>0.005). NSF had greater effectiveness compared to NaF in preventing decreases of pH and adhesion of S. mutans to the enamel surface, with statistically significant (p<0.001) differences.ConclusionNSF may be more effective than conventional fluorides in treating incipient caries lesions due to its remineralization and antibacterial actions.

<p>Remineralising Dentine Caries Using Sodium Fluoride with Silver Nanoparticles: An In Vitro Study</p>

“…25,26 Vieira Costa and Silva et al evaluated the antibacterial and cytotoxic activity of NSF against Streptococcus mutans, and they found that NSF was a bacteriostatic and bactericidal compound with a similar antimicrobial property to SDF and low toxicity to living cells. 27 Further research can be conducted to evaluate the effect of fluoridated PEG-AgNPs on multi-species cariogenic biofilm.…”

<p>Use of Silver Nanomaterials for Caries Prevention: A Concise Review</p>

“…33 Sodium fluoride with silver nanoparticles can act as a strategy for preventing and arresting caries. 17,18 Also, the addition of silver nanoparticles into restorative materials, such as adhesives and filling resins, can prevent secondary caries without compromising mechanical properties. 23,24 Silver nanoparticles can furthermore be utilized in orthodontic treatment to prevent initial caries by cooperating with adhesives, brackets, elastomeric ligatures, and removable retainers.…”

“…Sound enamel treated with nano silver fluoride has a similar value of microhardness to that of enamel treated with sodium fluoride. 18,19 The microhardness value of enamel caries treated with nano silver fluoride is higher than that of enamel caries treated with sodium fluoride. 20 However, the difference in the mineral content between nano silver fluorideand sodium fluoride-treated decayed enamel cannot be detected using optical coherence tomography.…”

“…13 Seven studies reported the use of nano silver fluoride solution for remineralizing early enamel caries and arresting dentin caries. [16][17][18][19][20][21][22] Thirty-one studies used resins with silver nanoparticles to prevent caries. Silver nanoparticles were added into restorative materials, such as filling resins and adhesives, to prevent secondary caries.…”

Efficiency of Nano Silver Fluoride on Prevention of Dental Caries on Intact Enamel Surface Assessed by Edx-Analysis (In Vitro Study)

“…On the other hand, Vieira Costa e Silva et al and Teixeira et al proved that the microhardness value of sound enamel surface treated by nanosilver fluoride is similar to that treated with sodium fluoride. (29,30) Furthermore, two separate clinical trials by Tirupathi et al and Dos Santos et al concluded that nanosilver fluoride has no effect on arresting the dentin caries in children. (31,32) Accordingly, further clinical studies are needed to accurately assess the nanosilver fluoride as a preventive measure.…”