<i>In vitro</i> methane and gas production with inocula from cows and goats fed an identical diet

Mengistu, Genet; Hendriks, W.H.; Pellikaan, W.F.

doi:10.1002/jsfa.8597

Cited by 3 publications

(9 citation statements)

References 34 publications

(59 reference statements)

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“…Extensive variability among individual animals with respect to the microbial species composition of the rumen, known as host individuality, is observed . Different types of substrates would cause different rumen microbial communities, and there were still variations even under uniform feeding and management conditions. , However, the differences in rumen microbial communities, which cause different fermentation efficiencies, are still unclear, and it is worth exploring these differences in subsequent studies. Some prior studies have found that the anaerobic fermentation mode of mixed inoculation could improve the efficiency of in vitro ruminal fermentation. , In the current study, the coinoculum group inoculated with biomass-normalized rumen content from goats and cows was investigated.…”

Section: Results and Dscussionmentioning

confidence: 99%

“…Some studies have demonstrated that the fermentative capacity of cows is higher than the fermentative capacity of goats. However, other studies reported different viewpoints . As seen from different results, a standardized in vitro ruminal fermentation system and experimental process are urgently needed to develop rumen microflora as effective resources for degrading cellulose.…”

Section: Results and Dscussionmentioning

confidence: 99%

“…However, other studies reported different viewpoints. 34 As seen from different results, a standardized in vitro ruminal fermentation system and experimental process are urgently needed to develop rumen microflora as effective resources for degrading cellulose. The RUSITEC was partially improved as the fermentative system used in this study, and it is now equipped with a completely automated control system that allows it to inoculate 1 L of rumen inoculum and sustain long-term and stable anaerobic fermentation conditions for in vitro ruminal fermentation.…”

Section: ■ Results and Dscussionmentioning

confidence: 99%

“…36 Different types of substrates would cause different rumen microbial communities, and there were still variations even under uniform feeding and management conditions. 10,34 However, the differences in rumen microbial communities, which cause different fermentation efficiencies, are still unclear, and it is worth exploring these differences in subsequent studies. Some prior studies have found that the anaerobic fermentation mode of mixed inoculation could improve the efficiency of in vitro ruminal fermentation.…”

Section: ■ Results and Dscussionmentioning

confidence: 99%

See 3 more Smart Citations

Metagenomic Analysis of in Vitro Ruminal Fermentation Reveals the Role of the Copresent Microbiome in Plant Biomass Degradation

Shi

Zhang

Wang

et al. 2022

J. Agric. Food Chem.

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In vitro ruminal fermentation is considered an efficient way to degrade crop residue. To better understand the microbial communities and their functions during in vitro ruminal fermentation, the microbiome and short chain fatty acid (SCFA) production were investigated using the metagenomic sequencing and rumen simulation technique (RUSITEC) system. A total of 1677 metagenome-assembled genomes (MAGs) were reconstructed, and 298 MAGs were found copresenting in metagenomic data of the current work and 58 previously ruminal representative samples. Additionally, the domains related to pectin and xylan degradation were overrepresented in the copresent MAGs compared with total MAGs. Among the copresent MAGs, we obtained 14 MAGs with SCFA-synthesis-related genes positively correlated with SCFA concentrations. The MAGs obtained from this study enable a better understanding of dominant microbial communities across in vivo and in vitro ruminal fermentation and show promise for pointing out directions for further research on in vitro ruminal fermentation.

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Section: Results and Dscussionmentioning

confidence: 99%

Section: Results and Dscussionmentioning

confidence: 99%

Section: ■ Results and Dscussionmentioning

confidence: 99%

Section: ■ Results and Dscussionmentioning

confidence: 99%

See 2 more Smart Citations

Metagenomic Analysis of in Vitro Ruminal Fermentation Reveals the Role of the Copresent Microbiome in Plant Biomass Degradation

Shi

Zhang

Wang

et al. 2022

J. Agric. Food Chem.

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“…However, the control of fermentation environment in vitro is one of the main factors leading to various results. Otherwise, it was confirmed that the rumen fermentation efficiency of cows was higher than that of goats, there were still follow-up studies that obtained the opposite results and it was difficult to give a reasonable explanation 30 . It could be seen that a standardized in vitro ruminal fermentation system and experimental process are urgently needed to develop rumen microflora as effective resources for degrading cellulose.…”

Section: Rusitecmentioning

confidence: 98%

Copresent microbiome and short-chain fatty acids profiles of plant biomass utilization in rumen simulation technique system

Shi

Zhang

Wang

et al. 2022

Preprint

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Rumen contents have considerable utility in converting plant biomass to short-chain fatty acids (SCFAs) when used as inoculum for in vitro fermentation. To better understand the microbial communities and their functions when in vitro ruminal fermentation, the microbiome and SCFAs production were investigated using rumen simulation technique (RUSITEC) system which was inoculated/co-inoculated with rumen contents from goat and cow. This study reconstructed 1677 microbial metagenome-assembled genomes (MAGs) from metagenomic sequencing. The copresent microbiome containing 298 MAGs were found in metagenomic data of these contents and previous ruminal representative samples. These copresent MAGs were overrepresented in decomposing various substrates, especially pectin and xylan. Additionally, the SCFAs productions in RUSITEC were linked with copresent MAGs. Copresent MAGs obtained from this study shows promise to point out the direction for further research on in vitro ruminal fermentation, and enables a better understanding of rumen microbiotal structures and functions under in vitro condition.

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A new equipment for continuous measurement of methane production in a batch in vitro rumen system

Braidot

Sarnataro

Romanzin

et al. 2022

Animal Physiology Nutrition

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A new rumen batch fermentation system that allows continuous measures of total gas (GP) and methane production (MP) was tested. The fermentation system is composed of glass bottles connected to gas counters (Ritter Apparatebau GmbH & Co. KG) and an infrared gas analyser that measures the methane concentration. The system allows direct and continuous measurement of GP and MP for accurate kinetic studies. The aim of the work was to test the rumen fermentation system and compare the GP and MP kinetics obtained. Barley meal (BM), alfalfa hay (AH), corn silage (CS), and soya bean hulls (SH) were used as substrates in four consecutive fermentation runs. Cumulative volumes of GP and MP and the percentage of methane on total GP were recorded continuously until 48 h and average values at 1 h intervals were fitted with an exponential model with a lag phase reaching a good fit (R2 > 0.992). GP and MP reached the highest plateau levels for SH (1836 and 370 ml, respectively; p < 0.01) and the lowest for AH (1000 and 233 ml, respectively). The remaining substrates showed intermediate values. MP kinetics showed a discrete lag phase (from 0.09 to 1.12 h), whereas it was equal to zero for the total GP (except for SH). The methane concentration in gas flowing increased rapidly at the beginning of fermentation (from 0.35 to 0.95 h−1) and reached a plateau after approximately 8–12 h. In conclusion, the rumen fermentation system evaluated generates methane data comparable to those reported in the literature and allows simple continuous measurement of methane release throughout fermentation.

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In vitro methane and gas production with inocula from cows and goats fed an identical diet

Abstract: Goat inoculum showed higher fermentative activity with a concomitant higher CH production compared to cows. This difference highlights the ability of goats to better utilise browse species and other roughage types. © 2017 Society of Chemical Industry.

Cited by 3 publications

References 34 publications

Metagenomic Analysis of in Vitro Ruminal Fermentation Reveals the Role of the Copresent Microbiome in Plant Biomass Degradation

Metagenomic Analysis of in Vitro Ruminal Fermentation Reveals the Role of the Copresent Microbiome in Plant Biomass Degradation

Copresent microbiome and short-chain fatty acids profiles of plant biomass utilization in rumen simulation technique system

A new equipment for continuous measurement of methane production in a batch in vitro rumen system

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