<i>In Vitro</i> Free Radical Scavenging Activity of a Wild Edible Mushroom, <i>Sparassis crispa</i> (Wulf.) Fr., from North Western Himalayas, India

Joshi, Madhavi; Sagar, Anand

doi:10.1155/2014/748531

Cited by 8 publications

(3 citation statements)

References 25 publications

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“…were found to be potent antioxidants in the concentration range of 40-60 μ g/mL and 10-70 μ g/mL, respectively. However, a previous study reported that the radical scavenging activity of mushroom extracts was shown at 2.11 mg/mL [26]. The possible reasons for the varied results of antioxidant activities between the present and previous study might be due to the different extraction conditions, and the difference in the presence of phenolic compounds and other secondary metabolites [27–29].…”

Section: Discussionmentioning

confidence: 53%

Investigation of Antioxidant and Antimicrobial Activities of Different Extracts of Auricularia and Termitomyces Species of Mushrooms

Gebreyohannes

Nyerere

Bii

et al. 2019

The Scientific World Journal

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Mushrooms produce a variety of bioactive compounds that are known to have a potential source of antioxidant and antimicrobial properties. Natural antioxidants can protect against free radicals without any side effects. The purpose of this study was to evaluate the antioxidant and antimicrobial activities of Auricularia and Termitomyces extracts. Specimens of Auricularia and Termitomyces spp. were collected from Kakamega National Reserve Forest in Kenya. Specimens were identified, extracted, and screened for their antioxidant and antimicrobial activities using stable free radical DPPH and colorimetric bioassay methods, respectively. The antimicrobial activity of the extracts was tested against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, MRSA, Candida albicans, and Candida parapsilosis. The maximum scavenging activity of hot water extract of Auricularia spp. was observed at 70.4% with the IC50 value of 40 μg/mL. Of the three extracts of Termitomyces spp., 70% ethanol extract has shown the highest scavenging activity (63%) with the IC50 value of 50 μg/mL. Chloroform and hot water extracts of Auricularia have shown statistically significantly different antifungal activities against C. parapsilosis (df = 2, F = 22.49, p ≤ 0.05). Of all the organisms, S. aureus was highly susceptible to 70% ethanol and hot water extracts of Termitomyces spp. with minimum inhibitory concentration values of 0.67±0.29 mg/mL. S. aureus and E. coli were the most susceptible and resistant bacteria to the hot water extract, respectively. In conclusion, the extracts of Auricularia spp. and Termitomyces spp. have shown promising antimicrobial and antioxidant activities.

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Section: Discussionmentioning

confidence: 53%

Investigation of Antioxidant and Antimicrobial Activities of Different Extracts of Auricularia and Termitomyces Species of Mushrooms

Gebreyohannes

Nyerere

Bii

et al. 2019

The Scientific World Journal

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“…All of the mushroom extracts in the current investigation had significant FRS activities in doses ranging from 10 g/mL to 70 g/mL. On the other hand, a prior investigation found that the FRS activity of mushroom extracts was 2.11 mg/mL [ 39 ]. The existence of numerous different secondary metabolites and differences in the quantity and potency of phenolic compounds are likely to be the primary causes of the inconsistent results of the free radical scavenging activities between the current report and earlier ones [ 40 – 42 ].…”

Section: Discussionmentioning

confidence: 75%

Wild Mushrooms: Potential Natural Sources of Antioxidant and Anti‐Quorum Sensing Bioactive Compounds for Medical Applications

Gebreyohannes,

Sbhatu,

Nyerere

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Objective. This study was aimed at determining the antioxidant, anti-quorum sensing, and in vitro cytotoxic activities of five wild mushroom extracts. Methods. Wild mushrooms of Auricularia auricula-judae, Termitomyces umkowaani, Trametes elegans, Trametes versicolor, and Microporus xanthopus were collected from Arabuko-Sokoke and Kakamega National Forests, in Kenya. Specimens were identified and extracted using chloroform (CHL), 70% ethanol (Eth), and hot water (HW) solvents. Antioxidant and cytotoxic activities of the extracts were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Vero cell lines, respectively, while anti-quorum sensing activities were tested against Chromobacterium violaceum. All data were compared using relevant descriptive and inferential statistics at a significance level of p ≤ 0.05. Results. A total of 35 wild mushrooms were collected, identified, and classified into 14 genera. Among screened mycochemicals, fatty acids, flavonoids, polyphenols, and saponins were detected at higher concentrations. The highest free radical scavenging activities of A. auricula-judae, T. umkowaani, T. elegans, and T. versicolor were observed in 70% Eth extract with the percentage values of 76.40 ± 0.12%, 68.40 ± 0.01%, 62.40 ± 0.07%, and 66.40 ± 0.04%, respectively, whereas the HW extract of Microporus xanthopus showed free radical scavenging activity at 65.90 ± 0.02%. None of the extracts, at the tested concentrations (up to 1000 µg/mL), had shown cytotoxic activity against the Vero cell line. The HW extract of T. umkowaani and the 70% Eth extract of T. versicolor showed a statistically significant difference in the inhibitory activity of violacein production against C. violaceum at the concentration of 200 µg/mL. Conclusions. The antioxidant activity of wild mushrooms can help to tackle the diseases caused by free radicals. The anti-quorum sensing potential of wild mushrooms could also provide future alternatives to conventional drug therapies cost-effectively. Further detailed chemistry of the bioactive compounds and their possible mechanisms of action responsible for the observed antioxidant and anti-quorum sensing activities are needed.

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“…In particular, β-glucan can prevent and heal common health problems such as diabetes, cancer, wound healing, as well as immune system and cytokine induction [ 1 , 6 , 12 , 13 , 14 , 15 ]. In addition, phenolic compounds, anti-fungal substances, and other S. crispa extracts may be used as anti-oxidant or anti-fungal agents [ 3 , 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Sparassis crispa in Medical Therapeutics: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

Ngoc

Lee

2018

IJMS

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In this study, we investigated the therapeutic potential and medical applications of Sparassis crispa (S. crispa) by conducting a systematic review of the existing literature and performing a meta-analysis. The original efficacy treatment of the mushroom extract is considered primarily and searched in electronic databases. A total of 623 articles were assessed, 33 randomized controlled experiments were included after the manual screening, and some papers, review articles, or editorials that did not contain data were excluded. A comparative standard means difference (SMD) and a funnel plot between control and S. crispa groups were used as parameters to demonstrate the beneficial effects of S. crispa for diabetes and cancer treatment, as well as anti-inflammatory, anti-fungal and antioxidant activities. The meta-analysis was carried out using Review Manager 5.1 software. Although for therapeutic diabetes there was heterogeneity in the subgroup analysis (I2 = 91.9%), the overall results showed statistically significant SMDs in major symptoms that decreased serum insulin levels (SMD = 1.92, 95% CI (1.10, 2.75), I2 = 0%), wound rates (SMD = 3.55 (2.56, 4.54), I2 = 40%) and contributions to an increase in nutrient intake content (SMD = 0.32 (−0.15, 0.78), I2 = 0%). Simultaneously, the study confirmed the utility of S. crispa treatment in terms of not only anti-cancer activity (reduction of tumor activity and survival of cancer cells I2 = 42 and 34%, respectively) but also anti-inflammatory, anti-fungal and antioxidant activities (I2 = 50, 44, and 10%, respectively). Our findings suggest that S. crispa extracts are useful for prevention and treatment of human diseases and might be the best candidates for future medicines.

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In Vitro Free Radical Scavenging Activity of a Wild Edible Mushroom, Sparassis crispa (Wulf.) Fr., from North Western Himalayas, India

Cited by 8 publications

References 25 publications

Investigation of Antioxidant and Antimicrobial Activities of Different Extracts of Auricularia and Termitomyces Species of Mushrooms

Investigation of Antioxidant and Antimicrobial Activities of Different Extracts of Auricularia and Termitomyces Species of Mushrooms

Wild Mushrooms: Potential Natural Sources of Antioxidant and Anti‐Quorum Sensing Bioactive Compounds for Medical Applications

Effects of Sparassis crispa in Medical Therapeutics: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

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