In vitro fermentation characteristics of diets with different forage/concentrate ratios: comparison of rumen and faecal inocula

Abstract

The results indicate that both faeces and rumen fluid from sheep have the potential to be used as inoculum for the in vitro gas production technique.

Influence of Yeast Fermented Cassava Chip Protein (YEFECAP) and Roughage to Concentrate Ratio on Ruminal Fermentation and Microorganisms Using <italic>In vitro</italic> Gas Production Technique

“…Similar to the current experiment, an enhanced propionate concentration was found in the rumen of cows fed high-grain as compared with high-forage diets (Sutton et al, 2003; Calabrò et al, 2008; Zicarelli et al, 2011). Surprisingly, Moorby et al (2006) reported linear increases in total VFA and butyrate concentrations and a decrease in acetate with increasing proportion of concentrate in dietary DM, but the concentration of propionate was not affected.…”

Effects of replacing soybean meal with dried rumen digesta on feed intake, digestibility of nutrients, rumen fermentation and nitrogen use efficiency in Thai cattle fed on rice straw

“…In this study, it could be related to the considerable amounts of semi-digested material and/or to unknown factors in DRD that enhanced rumen microorganisms (∅rskov, 1994;Liu et al, 2002). For example, the biological value of microorganisms in the DRD was found to be high, high minerals and B-vitamin and no anti-physiological factors (Okpanachi et al, 2010;Zicarelli et al, 2011) as well as end-products of rumen fermentation were contained in DRD, thus may result in efficient digestion of aNDF. The results are in agreement with previous studies in in vitro, supplementation of 8% DRD showed higher in vitro true digestibility (11.5%) than no DRD supplemented group (Cherdthong and Wanapat, 2013a).…”

Effect of Crude Protein Levels in Concentrate and Concentrate Levels in Diet on <i>In vitro</i> Fermentation

“…Pure chemicals of acetate, propionate and butyrate purchased from Sigma-Aldrich were used as external standards. The area of each VFA response was compared with those of the external standards (Zicarelli et al, 2011). …”

“…It can be determined by several methods such as in vivo , in situ , and in vitro techniques. In vivo method is laborious and requires a relatively large number of animals (Zicarelli et al, 2011). While in situ method has the advantage that it uses the actual rumen environment to measure feed digestion, it also has the disadvantage that it is expensive in terms of labour and analytical costs, and measures feed disappearance but not the actual amount of fermented substrate (Tagliapietra et al, 2011), primary and secondary losses of particulate matter as well as the differences between the level of microbial activity inside the in situ bags and that in the rumen (Tahir et al, 2013).…”