<i>In vitro</i> expression of cytokeratin 18, 19 and tube formation of adipose‐derived stem cells induced by the breast epithelial cell line <scp>HBL</scp>‐100

Yang, Jie; Xiong, Lingyun; Wang, Rongrong; Yuan, Quan; Xia, Yun; Sun, Jiaming; Horch, Raymund E.

doi:10.1111/jcmm.12673

Cited by 20 publications

(15 citation statements)

References 25 publications

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“…Cell cytokeratin (CK) is a main cytoskeletal protein in the keratinocyte that is mainly distributed in the epithelial cells, maintaining the integrity and continuity of the epithelial tissue is the major function of such type of structural protein [ 11 , 12 ]. Studies have found that CK is highly conserved and differentiated, closely related to the proliferation and differentiation of epithelial cells [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of relevant prognostic values of cytokeratin 20 and cytokeratin 7 expressions in lung cancer

et al. 2017

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Lung cancer is one of the most common malignant tumors harmful to human health. Cytokeratin (CK) is highly conserved and differentiated related to the proliferation and differentiation of epithelial cells. The aim of the study was to explore expressions of CK20 and CK7 and corresponding prognostic values in patients with lung cancer. Our study included 258 cases of patients confirmed with lung cancer. Expressions of CK20 and CK7 mRNA and protein were detected using real-time quantitative PCR (qRT-PCR) and Western blot, respectively, followed by the performance of immunohistochemistry staining. Associations of CK20 and CK7 with the clinical parameters and prognosis of lung cancer patients were further analyzed. There were obvious differences regarding the positive expression of CK20 in different T stage, lymph node metastasis, invasion, size, and clinical stage subgroups; besides, significant differences in the positive expression of CK7 were also observed in subgroups of different sex, age, lymph node metastasis, invasion, and differentiation. Furthermore, effects of age, smoking, T stage, lymph node metastasis and invasion, size, and CK7 expressions were significant on the survival of patients (all P<0.05). Multivariate analysis revealed that lymph node metastasis, T stage, and CK7 expression were independent risk factors for poor prognosis of involved patients (all P<0.05), while age, smoking, and invasion had no marked relation to the survival time of patients with lung cancer (all P>0.05). Positive CK20 and CK7 expressions are detected in patients with lung cancer; positive expression of CK7 associated with pathological features of lymph node metastasis and T stage may be independent clinical parameters for poor prognosis of patients with lung cancer.

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Section: Introductionmentioning

confidence: 99%

Identification of relevant prognostic values of cytokeratin 20 and cytokeratin 7 expressions in lung cancer

et al. 2017

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“…HIF is activated as a result of a drop in pO 2 [24]. Cytokeratin (CK) is a marker for epithelial cells [25, 26]. The intestinal epithelial cells play an important critical role in the immune response within the intestinal tissue and they are involved in the responses to hypoxic situation [27].…”

Section: Introductionmentioning

confidence: 99%

Partial Oxygen Pressure Affects the Expression of Prognostic Biomarkers HIF‐1 Alpha, Ki67, and CK20 in the Microenvironment of Colorectal Cancer Tissue

Zhang

Xi³

et al. 2016

Oxidative Medicine and Cellular Longevity

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Hypoxia is prognostically important in colorectal cancer (CRC) therapy. Partial oxygen pressure (pO2) is an important parameter of hypoxia. The correlation between pO2 levels and expression levels of prognostic biomarkers was measured in CRC tissues. Human CRC tissues were collected and pO2 levels were measured by OxyLite. Three methods for tissue fixation were compared, including formalin, Finefix, and Finefix-plus-microwave. Immunohistochemistry (IHC) staining was conducted by using the avidin-biotin complex technique for detecting the antibodies to hypoxia inducible factor-1 (HIF-1) alpha, cytokeratin 20 (CK20), and cell proliferation factor Ki67. The levels of pO2 were negatively associated with the size of CRC tissues. Finefix-plus-microwave fixation has the potential to replace formalin. Additionally, microwave treatment improved Finefix performance in tissue fixation and protein preservation. The percentage of positive cells and gray values of HIF-1 alpha, CK20, and Ki67 were associated with CRC development (P < 0.05). The levels of pO2 were positively related with the gray values of Ki67 and negatively related with the values of HIF-1 alpha and CK20 (P < 0.05). Thus, the levels of microenvironmental pO2 affect the expression of predictive biomarkers HIF-1 alpha, CK20, and Ki67 in the development of CRC tissues.

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“…Aufgrund ihrer Multipotenz, d. h. ihrer Differenzierungsfähigkeit in unterschiedliche Zelllinien, gelten sie als potente Kandidaten für eine Vielzahl von Anwendungen in der Regenerativen Medizin, wie u. a. im Knochen [51] oder Muskel Tissue Engineering [52]. Weiterhin können sie für die Hautgewebszüchtung eingesetzt werden [53] und können durch die Kokultivierung mit Mammaepithelzellen epithelial differenziert werden [54].…”

Section: Diskussionunclassified

Entwicklung eines neuen Zellisolationsverfahrens zur Erforschung der Mammakarzinompathogenese und -angiogenese für experimentelle in vitro und in vivo Assays

Weigand

Tasbihi

Strissel

et al. 2017

Handchir Mikrochir plast Chir

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Breast cancer is the world's most common cancer among women. Autologous lipotransfer is increasingly used for breast reconstruction following surgical removal of the tumour. In cell-assisted lipotransfer, the transplant is enriched with stem cells from adipose tissue (ADSC). Despite positive clinical results, there are some concerns regarding oncological safety due to transplanted stem cells. To date there are only a few breast cancer studies using primary cells from the same patient to enable further investigation into the complexity of cell-cell interactions in breast cancer in an experimental setting. We performed literature research on the topic of autologous lipotransfer. 5 different cell types (epithelial, mesenchymal cells, ADSC, endothelial cells, endothelial progenitor cells) were isolated from mammary (carcinoma) tissue or blood and were subsequently characterised for gene and protein expression as well as functional properties. The arteriovenous (AV) loop model in the rat was evaluated as a possible in vivo model for breast cancer pathogenesis and angiogenesis in this study. The literature provided evidence for an in-vitro interaction between ADSC and cells of the mammary (carcinoma) tissue. In some clinical studies, certain subgroups of patients appeared to be exposed to an increased risk of tumour recurrence after lipotransfer, but in most studies no correlation between lipotransfer and tumour recurrence was found. Different cell populations, which differed significantly in terms of surface markers, gene expression and functional properties, were isolated from tissue of the same patient. Axial vascularised tissue was successfully generated in the AV loop model. In this study we were able to isolate different cell populations from the same patient, which reflect the heterogeneity of the tumour tissue. This enables a precise analysis of cell-cell interactions and their effects on tumour angiogenesis and pathogenesis in breast cancer. In combination with the AV loop model, this offers new possibilities to generate vascularised mammary carcinoma tissue as well as healthy mammary gland tissue in vivo as an optimal model for the clinical setting.

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In vitro expression of cytokeratin 18, 19 and tube formation of adipose‐derived stem cells induced by the breast epithelial cell line HBL‐100

Cited by 20 publications

References 25 publications

Identification of relevant prognostic values of cytokeratin 20 and cytokeratin 7 expressions in lung cancer

Identification of relevant prognostic values of cytokeratin 20 and cytokeratin 7 expressions in lung cancer

Partial Oxygen Pressure Affects the Expression of Prognostic Biomarkers HIF‐1 Alpha, Ki67, and CK20 in the Microenvironment of Colorectal Cancer Tissue

Entwicklung eines neuen Zellisolationsverfahrens zur Erforschung der Mammakarzinompathogenese und -angiogenese für experimentelle in vitro und in vivo Assays

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