In vitro evidence for a neurite growth‐promoting activity in trembler mouse serum

Abstract

Basal lamina components, such as heparan sulfate proteoglycan (HSPG) and laminin play an important role in neuritic outgrowth for CNS and PNS neurons in culture. The mutant mouse 'Trembler' is characterized by hypomyelinization and production of an excess of basal lamina layers around Schwann cells in peripheral nerves. In order to analyse whether or not the serum of the mutant animals contains neurite outgrowth-promoting factors, we cultured rat spinal cord neurons in the presence of Trembler serum. Under these conditions, the outgrowth of neurites was increased approx. 2 times as compared to control serum. Trembler serum induces neuritic outgrowth characterized both by an increase in number of primary neurites emerging from the nerve cell body as well as by an increase in peripheral branching of neurites. To characterize the factors implicated in this increase we added antibodies directed against HSPG or laminin to the mutant serum. As a result, the increase in neuritic outgrowth was reduced or abolished in both cases. Trembler effect on neurite growth disappeared when the number of the non-neuronal cells was reduced, suggesting that the mutant serum did not act directly on neurons but by the intermediary action of non-neuronal cells.

In vivo Proliferative Pattern of Trembler Hypomyelinating Schwann Cells Is Modified in Culture: An Experimental Analysis

“…Trembler serum, in contrast with normal mouse serum, contained, as we have previously suggested [19], a powerful mitosis-stimulating activity which rapidly in creased the proportion of dividing Schwann cells.…”

“…In a previous paper, we have suggested that the in crease of neurite outgrowth initiated by Trembler serum in embryonic rat spinal cord cultures is enhanced by the stimulation of nonneuronal cells, fibroblasts and mainly glial cells, which multiply initially [19].…”