<i>In vitro</i> evaluation of poly (lactic‐<i>co</i>‐glycolic acid)/polyisoprene fibers for soft tissue engineering

Marques, Douglas Ramos; Santos, Luís Alberto Loureiro dos; O’Brien, Marie; Cartmell, Sarah H.; Gough, Julie E.

doi:10.1002/jbm.b.33796

Cited by 14 publications

(4 citation statements)

References 71 publications

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“…The impact of antibody conjugation on the properties of the nanoparticles was further assessed by FTIR, TGA, and polarity measurements. The FTIR spectrum for bare PLGA nanoparticles is shown in Figure 1 D and is similar to results from previous studies 25 , 26 . After antibody conjugation, a peak appeared at 1600 cm -1 to 1730 cm -1 from the carbonyl groups of DSPE-PEG-maleimide and C-S bonding was observed at 500 cm -1 to 600 cm -1 .…”

Section: Resultssupporting

confidence: 86%

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation

et al. 2021

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To improve the treatment of psoriasiform inflammation, we developed actively targeted nanocarriers loaded with the phosphodiesterase 4 inhibitor AN2728. Methods: Phospholipid-poly(lactic- co -glycolic acid) nanohybrids were prepared and conjugated with monovalent anti-desmoglein 3 antibody to bind keratinocytes. Results: The actively targeted nanohybrids were 229 nm in mean size with a nearly neutral surface charge. Flow cytometry and confocal microscopy showed a 9-fold increase in keratinocyte uptake of targeted nanohybrids relative to non-targeted nanoparticles. The nanoparticles localized mainly in lysosomes after internalization. AN2728-loaded antibody-conjugated nanocarriers inhibited cytokine/chemokine overexpression in activated keratinocytes without affecting cell viability. The targeted nanohybrids also suppressed neutrophil migration by reducing CXCL1 and CXCL2 release from keratinocytes. Following subcutaneous administration in mice, the nanohybrids distributed to the epidermis and hair follicles. In a psoriasis-like skin mouse model, the actively targeted nanoparticles were superior to free drug and non-targeted nanoparticles in mitigating skin inflammation. Intervention with the targeted nanosystem reduced the epidermal thickness of the psoriasiform lesion from 191 to 42 µm, decreased the Psoriasis Area Severity Index by 74%, restored barrier function, and returned chemokine levels to baseline. Conclusions: Our developed nanosystem was safe and demonstrated efficient targeting properties for the treatment of cutaneous inflammation.

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Section: Resultssupporting

confidence: 86%

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation

et al. 2021

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“…El trabajo desarrollado por Schopf et al evalua los stents en forma de espiral y por fragmentos a base PLGA y poli-isopreno en un modelo animal de conejos adultos blancos (11). Este estudio corrobora histológicamente los resultados obtenidos por Marques et al sobre el uso experimental de fibras de la misma mezcla de polímeros en ingeniería de tejidos (11,18). En el trabajo de Schopf et al se identifica la importancia de reducir el espesor de los stents bioabsorbibles a fin de disminuir las manifestaciones clínicas y la respuesta inflamatoria asociada a la implantación del stent (11).…”

Section: Medicinaunclassified

Stents bronquiales bioabsorbibles y factores que afectan su velocidad de degradación

Colmenares-Roldan

Palacio

2018

CES Med

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Las causas que pueden conducir a la obstrucción de la vía aérea central pueden ser de origen funcional, por obstrucción de la luz, por lesión orgánica parietal o compresión extrínseca; a su vez, también pueden agruparse en obstrucciones malignas y no malignas. Cuando una obstrucción reduce el 50 % de la luz de la vía aérea causa síntomas debilitantes y es una de las indicaciones para implantar un stent bronquial. Los stents bronquiales actualmente disponibles son una solución incompleta para las obstrucciones de las vías aéreas. Por otra parte, un stent bronquial ideal debe cumplir con muchas características, tales como ser biocompatible, en muchos casos bioabsorbible, radio opaco, que no genere reacción inflamatoria, tener características similares a las de la vía aérea para disminuir la acumulación de secreciones, entre otras. Por esta razón los stents bronquiales bioabsorbibles se presentan como una alternativa atractiva que ofrece ciertas ventajas, aunque aún se encuentran en desarrollo. El presente artículo busca describir los avances alrededor de los stents bronquiales bioabsorbibles y los factores que afectan la degradación de los polímeros con los cuales se han fabricado.

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“…Studies have shown that the combination of the two materials increases the mechanical strength of the polymer when compared to PLGA fibers alone and has been used successfully. 8,10,11 However, the addition of spherical microparticles to CaP, despite increasing its macroporosity, does not allow a pattern of channels and interconnectivity between the pores, necessary for better tissue penetration and new bone formation. 12 Studies have incorporated resorbable polymer fibers, such as polyglactin, 13 poly(epsilon-caprolactone) and polylactic acid, 5 with the aim of increasing the mechanical resistance and the amount of interconnected macropores, which are important characteristics when it is used in medical and dental reconstructions, in mechanical stress places, in large bone reconstructions and sites of thin and delicate bone structure.…”

Section: Introductionmentioning

confidence: 99%

“…Studies have shown that the combination of the two materials increases the mechanical strength of the polymer when compared to PLGA fibers alone and has been used successfully. 8,10,11…”

Section: Introductionmentioning

confidence: 99%

Histological and structural evaluation of growth hormone and PLGA incorporation in macroporous scaffold of α-tricalcium phosphate cement

et al. 2018

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An in vivo study was conducted to evaluate the effects of the incorporation of fibers of poly(lactic acid-co-glycolic acid, PLGA) and poly(isoprene) blend and recombinant human growth hormone (rhGH) in a macroporous scaffold of a-tricalcium phosphate cement (a-TCP) samples inserted into calvarial defects (8 mm in diameter) of 48 Wistar rats. The samples of a-TCP þ PLGA/poly(isoprene) blend fibers were also submitted to a mechanical test of flexural strength. The animals of the different experimental groups [1] a-TCP (n ¼ 6); [2] a-TCP þ PLGA/poly(isoprene) blend fibers (n ¼ 6); [3] a-TCP þ rhGH, (n ¼ 6) and [4] a-TCP þ PLGA/poly(isoprene) blend fibers þ rhGH, (n ¼ 6) (the numbers within square brackets identify the experimental groups), after two weeks (subdivision "a") and four weeks (subdivision "b"), were euthanized and the implants removed for histological analysis. There was no statistical difference (p > 0.05) between the samples with and without fibers in the mechanical test. Light microscopy revealed good integration of the material in the host tissue, represented by tissue penetration into the macropores and adequate angiogenesis. In the two-week period, the groups [3a] and [4a] were significantly superior (p < 0.05) to the other groups with regard to angiogenesis and bone neoformation. In the four-week period, the group [3b] was significantly superior (p < 0.05) to the other groups with regard to bone neoformation. We conclude that the macroporous a-TCP scaffold used in this study has low mechanical resistance, is biocompatible and has significantly improved the osteoconductive capacity when rhGH is incorporated into its structure.

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In vitro evaluation of poly (lactic‐co‐glycolic acid)/polyisoprene fibers for soft tissue engineering

Abstract: Abstract:The polymeric blend of Poly (Lactic-co-Glycolic Acid) (PLGA) and Polyisoprene

Cited by 14 publications

References 71 publications

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation

Stents bronquiales bioabsorbibles y factores que afectan su velocidad de degradación

Histological and structural evaluation of growth hormone and PLGA incorporation in macroporous scaffold of α-tricalcium phosphate cement

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