<i>In vitro</i>evaluation of functionalized decellularized muscle scaffold for<i>in situ</i>skeletal muscle regeneration

Shapiro, Lindsey; Elsangeedy, Ebrahim; Lee, Hyeongjin; Atala, Anthony; Yoo, James J.; Lee, Sang Jin; Ju, Young Min

doi:10.1088/1748-605x/ab229d

Cited by 21 publications

(11 citation statements)

References 59 publications

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“…Decellularised scaffolds are derived from xenogeneic, allogenic or autogenic skeletal muscle tissue [24][25][26]. Once deceullularisation has removed cellular material, the remaining ECM retains the native 3D microstructure, molecular composition and growth factors that support skeletal muscle regeneration [25].…”

Section: Decellularised Scaffoldsmentioning

confidence: 99%

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Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

Langridge

Butler

2021

J Mater Sci: Mater Med

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Skeletal muscle is capable of regeneration following minor damage, more significant volumetric muscle loss (VML) however results in permanent functional impairment. Current multimodal treatment methodologies yield variable functional recovery, with reconstructive surgical approaches restricted by limited donor tissue and significant donor morbidity. Tissue-engineered skeletal muscle constructs promise the potential to revolutionise the treatment of VML through the regeneration of functional skeletal muscle. Herein, we review the current status of tissue engineering approaches to VML; firstly the design of biocompatible tissue scaffolds, including recent developments with electroconductive materials. Secondly, we review the progenitor cell populations used to seed scaffolds and their relative merits. Thirdly we review in vitro methods of scaffold functional maturation including the use of three-dimensional bioprinting and bioreactors. Finally, we discuss the technical, regulatory and ethical barriers to clinical translation of this technology. Despite significant advances in areas, such as electroactive scaffolds and three-dimensional bioprinting, along with several promising in vivo studies, there remain multiple technical hurdles before translation into clinically impactful therapies can be achieved. Novel strategies for graft vascularisation, and in vitro functional maturation will be of particular importance in order to develop tissue-engineered constructs capable of significant clinical impact.

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Section: Decellularised Scaffoldsmentioning

confidence: 99%

“…Murine C2C12 are immortalised myoblasts derived from murine satellite cells and are widely used in vitro [24,43,69,[80][81][82][83][84]. They readily proliferate and differentiate under differing serum conditions and so are a useful Fig.…”

Section: Progenitor Cells Populations For Skeletal Muscle Tissue Engineeringmentioning

confidence: 99%

Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

Langridge

Butler

2021

J Mater Sci: Mater Med

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“…FGF, PDGF, and HGF promote the activation and proliferation of myogenic progenitor cells [ 101 ]. Insulin-like growth factor (IGF-I) plays an important role in all phases of satellite cell myogenesis by controlling the migration, proliferation, and differentiation of muscle satellite/progenitor cells [ 102 ].…”

Section: Customized Functionalization By Click Chemistry Of Composmentioning

confidence: 99%

Smart ECM-Based Electrospun Biomaterials for Skeletal Muscle Regeneration

et al. 2020

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The development of smart and intelligent regenerative biomaterials for skeletal muscle tissue engineering is an ongoing challenge, owing to the requirement of achieving biomimetic systems able to communicate biological signals and thus promote optimal tissue regeneration. Electrospinning is a well-known technique to produce fibers that mimic the three dimensional microstructural arrangements, down to nanoscale and the properties of the extracellular matrix fibers. Natural and synthetic polymers are used in the electrospinning process; moreover, a blend of them provides composite materials that have demonstrated the potential advantage of supporting cell function and adhesion. Recently, the decellularized extracellular matrix (dECM), which is the noncellular component of tissue that retains relevant biological cues for cells, has been evaluated as a starting biomaterial to realize composite electrospun constructs. The properties of the electrospun systems can be further improved with innovative procedures of functionalization with biomolecules. Among the various approaches, great attention is devoted to the “click” concept in constructing a bioactive system, due to the modularity, orthogonality, and simplicity features of the “click” reactions. In this paper, we first provide an overview of current approaches that can be used to obtain biofunctional composite electrospun biomaterials. Finally, we propose a design of composite electrospun biomaterials suitable for skeletal muscle tissue regeneration.

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“…MnPNH 2 purity was determined by high performance liquid chromatography (HPLC), ultraviolet-visible (UV-Vis) spec troscopy, flame atomic absorption spectroscopy (FAAS), and mass spectroscopy. HPLC was run using a continuous ramping protocol with a gradient mix of ACN to 10mM NH 4…”

Section: Characterization Of Mnpnhmentioning

confidence: 99%

MRI method for labeling and imaging decellularized extracellular matrix scaffolds for tissue engineering

Szulc

Ahmadipour

Aoki

et al. 2019

Magnetic Resonance in Med

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Purpose To develop a facile method for labeling and imaging decellularized extracellular matrix (dECM) scaffolds intended for regenerating 3D tissues. Methods A small molecule manganese porphyrin, MnPNH2, was synthesized and used to label dECM scaffolds made from porcine bladder and trachea and murine whole lungs. The labeling protocol was optimized on bladder dECM, and imaging on a 3T clinical scanner was performed to assess reductions in T1 and T2 relaxation times. In vivo MRI was performed on dECM injected in the rat dorsum to verify sensitivity of detection. Toxicity assays for cell viability, metabolism, and proliferation were performed on human umbilical vein endothelial cells. The incorporation of MnPNH2 and its long‐term retention in dECM were assessed on transmission electron microscopy and ultraviolet absorbance of eluted MnPNH2 over time. Results All tissues, including thick whole 3D organs, were uniformly labeled and demonstrated high signal‐to‐noise on MRI. A nearly 10‐fold reduction in T1 was consistently obtained at a labeling dose of 0.4 mM, and even 0.2 mM provided sufficient contrast in vivo and ex vivo. No toxicity was observed up to 0.4 mM, the maximum tested. Binding studies suggested nonspecific association, and retention studies in the labeled whole decellularized lungs revealed less than 20% MnPNH2 loss over 30 days, the majority occurring in the first 3 days after labeling. Conclusion The proposed labeling method is the first report for visualizing dECM on MRI and has the potential for long‐term monitoring and optimization of dECM‐based organ tissue engineering.

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In vitroevaluation of functionalized decellularized muscle scaffold forin situskeletal muscle regeneration

Cited by 21 publications

References 59 publications

Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

Smart ECM-Based Electrospun Biomaterials for Skeletal Muscle Regeneration

MRI method for labeling and imaging decellularized extracellular matrix scaffolds for tissue engineering

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