<i>In Vitro</i>
            Evaluation of Biofilm Dispersal as a Therapeutic Strategy To Restore Antimicrobial Efficacy

Roizman, Dan; Vidaillac, Céline; Givskov, Michael; Yang, Liang

doi:10.1128/aac.01088-17

Cited by 21 publications

(17 citation statements)

References 15 publications

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“…In the present study, IPM was the most effective in S. putrefaciens and K. pneumoniae biofilm mass removal, however, total removal was not achieved, which is a critical aspect in biofilm control. In fact, antibiotics can promote significant killing of biofilm cells without causing their dispersal/removal ( Roizman et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from wild animals

Días

Borges

Oliveira

et al. 2018

PeerJ

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BackgroundThe “One Health” concept recognizes that human health and animal health are interdependent and bound to the health of the ecosystem in which they (co)exist. This interconnection favors the transmission of bacteria and other infectious agents as well as the flow of genetic elements containing antibiotic resistance genes. This problem is worsened when pathogenic bacteria have the ability to establish as biofilms. Therefore, it is important to understand the characteristics and behaviour of microorganisms in both planktonic and biofilms states from the most diverse environmental niches to mitigate the emergence and dissemination of resistance.MethodsThe purpose of this work was to assess the antibiotic susceptibility of four bacteria (Acinetobacter spp., Klebsiella pneumoniae, Pseudomonas fluorescens and Shewanella putrefaciens) isolated from wild animals and their ability to form biofilms. The effect of two antibiotics, imipenem (IPM) and ciprofloxacin (CIP), on biofilm removal was also assessed. Screening of resistance genetic determinants was performed by PCR. Biofilm tests were performed by a modified microtiter plate method. Bacterial surface hydrophobicity was determined by sessile drop contact angles.ResultsThe susceptibility profile classified the bacteria as multidrug-resistant. Three genes coding for β-lactamases were detected in K. pneumoniae (TEM, SHV, OXA-aer) and one in P. fluorescens (OXA-aer). K. pneumoniae was the microorganism that carried more β-lactamase genes and it was the most proficient biofilm producer, while P. fluorescens demonstrated the highest adhesion ability. Antibiotics at their MIC, 5 × MIC and 10 × MIC were ineffective in total biofilm removal. The highest biomass reductions were found with IPM (54% at 10 × MIC) against K. pneumoniae biofilms and with CIP (40% at 10 × MIC) against P. fluorescens biofilms.DiscussionThe results highlight wildlife as important host reservoirs and vectors for the spread of multidrug-resistant bacteria and genetic determinants of resistance. The ability of these bacteria to form biofilms should increase their persistence.

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Section: Discussionmentioning

confidence: 99%

Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from wild animals

Días

Borges

Oliveira

et al. 2018

PeerJ

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“…However, later investigations revealed that biofilm dispersal could induce by the availability of nutrient, oxygen limitations, and the presence of several chemicals 6 . New studies are beginning to address biofilm dispersal as a highly regulated active process for start of infection at new sites 7 .…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, typical in vitro studies suggest strategies to increase the efficacy of antibiotics against biofilms involves induction of biofilm dispersal 6 . This led to explore biofilm control by using dispersal in P. aeruginosa as well.…”

Section: Introductionmentioning

confidence: 99%

Comparison of biofilm dispersal approaches in Pseudomonas aeruginosa and evaluation of dispersed cells in acute infection mouse model

Ruhal

Winter

et al. 2019

Preprint

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Biofilm dispersal is biologically significant process to fully understand its consequences during biofilm based infections. The mechanism for biofilm dispersal may involve response to environmental cues and changes in intracellular secondary messengers. Considering range of cues for biofilm dispersal, it is significant to study if dispersed cells generated by different methods have similar phenotype. In the present study, we have compared four type of biofilm dispersal in P. aeruginosa based in response to environment cues (starvation and nutrient rich), c-di-GMP knockdown and signaling molecule NO. We have determined their dispersal efficiency, susceptibility of dispersed cells for antibiotics, their transcriptomic changes compared to planktonic and biofilm. We also determined if dispersed cells can cause acute infections in mouse models. In vitro experiments showed that NO and c-di-GMP based dispersal methods had high biofilm reduction efficiency of 50 and 75% respectively, however, the nutrient induced dispersion showed low efficiency (≈30%) and more tolerance to colistin.We also showed that in vitro dispersed cells induced >10-fold transcriptomic expression of genes (at significance level of p < 0.005) related to efflux pumps (mexCD-oprJ), antibiotic resistance (arnDET) in dispersed cells induced by NO and nutrient change, while denitrification pathway and virulence (T3SS, VreI, T2SS) genes in all dispersed cells compared to planktonic and biofilm state. When the ability of the dispersed cells was tested in mouse model of lung infection, c-di-GMP and NO based dispersed cells displayed enhanced infections and haematogenous spread to liver and spleen and higher mortality. Although degree of immune response (cytokines) does not differ based on phenotypes inoculated in our experimental conditions. Based on our data most efficient dispersal methods increase murine mortality. This indicates their capability of making biofilm associated infection more complicated. Our data encourage to be more careful for studies suggesting biofilm dispersal during treatment of biofilm infections.

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“…The objectives of this study were to 1) assess the potential for synergy of the unconventional antimicrobial combinations and 2) evaluate the potential of c-di-GMP mediated biofilm dispersal to increase antimicrobial efficacy; against biofilms of colistin susceptible and resistant strains of P. aeruginosa. Dispersal of biofilm utilizing this specific cellular mechanism has proven to be a promising strategy to treat biofilms of susceptible strains (Roizman, Vidaillac et al 2017). The question here is whether this strategy is effective against biofilms of resistant bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the publication "In-vitro evaluation of biofilm dispersal as a therapeutic strategy to restore antimicrobial efficacy" (Roizman, Vidaillac et al 2017).…”

Section: Dispersal As a Strategy To Restore Antibiotic Efficacymentioning

confidence: 99%

An in-vitro evaluation of C-di-GMP mediated biofilm dispersal as a potential strategy to restore antimicrobial efficacy

Roizman¹

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My time spent in graduate school was a memorable undertaking, full of adventure, excitement, ups and downs, and mainly, a tremendous learning experience. In that sense, first and foremost, I would like to thank my supervisor, Assoc. Prof. Yang Liang, for showing me the path to do great science, for his guidance and his encouragement. I would like to thank my mentor, Assoc. Prof. Scott Rice, for his important advice. I would also like to thank my co-supervisor Assoc. Prof. Tan Choon Hong for his assistance along the way. I am especially grateful to Dr. Céline Vidaillac. Words cannot express my sincere, heartfelt gratitude for all of her efforts to assist me in my scientific endeavor and my career. I would like to thank her for her guidance and inspiration, for being a beacon in stormy seas. I thank the director of SCELSE, Prof. Staffan Kjelleberg, for his vision, managing a unique and unusual interdisciplinary center, and for the opportunity to take part in cutting-edge science. Thanks to SCELSE graduate programme director, Assoc. Prof. Sanjay Swarup, for his continued support and confidence in me. I also thank my former supervisor, Prof. Yehuda Cohen, for providing me the opportunity to partake in this research experience. Thanks to all the people at IGS for supporting my research and me. I would like to thank all of Prof. Yang's group members, my fellow Ph.D. students and predecessors. A special thank you to Chin Joyce and Seng Zi Jing, for their continued assistance to my research. Thanks to my dear friends, II colleagues and former colleagues in SCELSE: To Dr. Damien Keogh, also for taking the time and proofreading my thesis. Asst. Prof. Enrico Marsili, Dr.

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In Vitro Evaluation of Biofilm Dispersal as a Therapeutic Strategy To Restore Antimicrobial Efficacy

Cited by 21 publications

References 15 publications

Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from wild animals

Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from wild animals

Comparison of biofilm dispersal approaches in Pseudomonas aeruginosa and evaluation of dispersed cells in acute infection mouse model

An in-vitro evaluation of C-di-GMP mediated biofilm dispersal as a potential strategy to restore antimicrobial efficacy

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