<i>In Vitro</i>
            Evaluation of Antimicrobial Peptides from the Black Soldier Fly (
            <i>Hermetia Illucens</i>
            ) against a Selection of Human Pathogens

Moll, Laurence Van; Smet, Jeroen De; Paas, Anne; Tegtmeier, Dorothee; Vilcinskas, Andreas; Cos, Paul; Campenhout, Leen Van

doi:10.1128/spectrum.01664-21

Cited by 36 publications

(41 citation statements)

References 81 publications

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“…The hemolysis analysis confirmed the non-toxicity suggested by HemoPred online software [ 153 ]. Similarly, the hemolytic results of cecropins are in close agreement with previous conclusions from this web server [ 154 ]. In silico screening of the properties of peptides from the genome of Lactobacillus casei HZ1, including bioinformatics analysis by HemoPI, allowed the identification of a highly active AMP against S. aureus [ 155 ] and an anticancer sequence [ 156 ], both with low hemolytic effects.…”

Section: Scheme and Scope Of Hemolytic Classifierssupporting

confidence: 91%

Traditional and Computational Screening of Non-Toxic Peptides and Approaches to Improving Selectivity

et al. 2022

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Peptides have positively impacted the pharmaceutical industry as drugs, biomarkers, or diagnostic tools of high therapeutic value. However, only a handful have progressed to the market. Toxicity is one of the main obstacles to translating peptides into clinics. Hemolysis or hemotoxicity, the principal source of toxicity, is a natural or disease-induced event leading to the death of vital red blood cells. Initial screenings for toxicity have been widely evaluated using erythrocytes as the gold standard. More recently, many online databases filled with peptide sequences and their biological meta-data have paved the way toward hemolysis prediction using user-friendly, fast-access machine learning-driven programs. This review details the growing contributions of in silico approaches developed in the last decade for the large-scale prediction of erythrocyte lysis induced by peptides. After an overview of the pharmaceutical landscape of peptide therapeutics, we highlighted the relevance of early hemolysis studies in drug development. We emphasized the computational models and algorithms used to this end in light of historical and recent findings in this promising field. We benchmarked seven predictors using peptides from different data sets, having 7–35 amino acids in length. According to our predictions, the models have scored an accuracy over 50.42% and a minimal Matthew’s correlation coefficient over 0.11. The maximum values for these statistical parameters achieved 100.0% and 1.00, respectively. Finally, strategies for optimizing peptide selectivity were described, as well as prospects for future investigations. The development of in silico predictive approaches to peptide toxicity has just started, but their important contributions clearly demonstrate their potential for peptide science and computer-aided drug design. Methodology refinement and increasing use will motivate the timely and accurate in silico identification of selective, non-toxic peptide therapeutics.

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Section: Scheme and Scope Of Hemolytic Classifierssupporting

confidence: 91%

Traditional and Computational Screening of Non-Toxic Peptides and Approaches to Improving Selectivity

et al. 2022

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“…Moreover, insects can also feed on substances with different levels of contaminations, so they synthesize AMPs to fight such infections and survive in dangerous conditions [ 66 ]. AMPs extracted from insects have the potential to fight the microorganisms that act as hazards to human health [ 66 , 67 ]. One of the most interesting insect species is Diptera Hermetia illucens , which is able to produce a number of AMPs, far superior to that of other insects [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of the Antibacterial Activity of the Peptide Fractions Extracted from the Hemolymph of Hermetia illucens (Diptera: Stratiomyidae)

Scieuzo

Giglio

Rinaldi

et al. 2023

Insects

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Antimicrobial peptides (AMPs) are a chemically and structurally heterogeneous family of molecules produced by a large variety of living organisms, whose expression is predominant in the sites most exposed to microbial invasion. One of the richest natural sources of AMPs is insects which, over the course of their very long evolutionary history, have adapted to numerous and different habitats by developing a powerful innate immune system that has allowed them to survive but also to assert themselves in the new environment. Recently, due to the increase in antibiotic-resistant bacterial strains, interest in AMPs has risen. In this work, we detected AMPs in the hemolymph of Hermetia illucens (Diptera, Stratiomyidae) larvae, following infection with Escherichia coli (Gram negative) or Micrococcus flavus (Gram positive) and from uninfected larvae. Peptide component, isolated via organic solvent precipitation, was analyzed by microbiological techniques. Subsequent mass spectrometry analysis allowed us to specifically identify peptides expressed in basal condition and peptides differentially expressed after bacterial challenge. We identified 33 AMPs in all the analyzed samples, of which 13 are specifically stimulated by Gram negative and/or Gram positive bacterial challenge. AMPs mostly expressed after bacterial challenge could be responsible for a more specific activity.

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“… A protected and distinct phenotype is adopted by some cells of biofilm. The response is biologically programmed and is not initiated due to limitations of nutrients [23].…”

Section: Antibiotics and Biofilmsmentioning

confidence: 99%

A review on the potential of antibacterial peptides to control biofilm resistance

Liaqat¹

2023

PAB

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Biofilms are comprised of communities of bacteria that are encased in the matrix. In biofilm, microbes become dormant and less sensitive to ordinary antibiotics. It is difficult for the antibiotics to penetrate and reach the depth of biofilm. These characteristics of biofilm make it 1000 times more resistant than planktonic microbes. Antibacterial peptides are used as an alternative of antibiotics to deal with the infections that are related to biofilm. Antibacterial peptides are small peptides that have broad action against Gram-positive and Gram-negative bacteria. Significant mechanisms of antibacterial peptides are: 1) degradation or disruption of the membrane potential of biofilms' embedded cell, 2) interruption of bacterial cells' signaling system, 3) degradation of polysaccharides and matrix of biofilm, 4) inhibition of the bacterial alarmone to avoid the stringent responses and 5) down-regulation of the genes that are responsible for biofilm formation and transportation of the binding proteins. The main purpose of this review article is to summarize the fresh material related to antibacterial peptides, their activity, and mechanism of action against the biofilm. Furthermore, health community and society can get new information regarding the suitable alternatives of ordinary antibiotics to control resistance in biofilms.

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In Vitro Evaluation of Antimicrobial Peptides from the Black Soldier Fly ( Hermetia Illucens ) against a Selection of Human Pathogens

Cited by 36 publications

References 81 publications

Traditional and Computational Screening of Non-Toxic Peptides and Approaches to Improving Selectivity

Traditional and Computational Screening of Non-Toxic Peptides and Approaches to Improving Selectivity

In Vitro Evaluation of the Antibacterial Activity of the Peptide Fractions Extracted from the Hemolymph of Hermetia illucens (Diptera: Stratiomyidae)

A review on the potential of antibacterial peptides to control biofilm resistance

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