<i>In Vitro</i> Evaluation of Antimicrobial Activity and Cytotoxicity of Different Nanobiotics Targeting Multidrug Resistant and Biofilm Forming Staphylococci

Mohamed, Mennatallah; Nasr, Maha; Elkhatib, Walid F.; Eltayeb, Wafaa N

doi:10.1155/2018/7658238

Cited by 35 publications

(22 citation statements)

References 50 publications

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“…A significant reduction in cell viability was detected at high concentration (5 and 10 mg) and high temperature (900 and 1000 ℃). It has been reported that increased biocidal activity could reduce cell viability because undesirable conditions around live cells are created 33 . Notably, calcined oyster shells (2.5 mg) treated at 750℃ showed good human body compatibility with higher number of live cells, indicating that the calcined oyster shells at this level and temperature can be an alternative antimicrobial agent.…”

Section: Resultsmentioning

confidence: 99%

Effects of calcination temperature on morphological and crystallographic properties of oyster shell as biocidal agent

Park

Sadeghi

Thanakkasaranee

et al. 2020

Int J Applied Ceramic Tech

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To develop biocompatible antimicrobial agent, oyster shell wastes were thermally calcined at different temperatures ranging from 300 to 1000 °C. The chemical compositions and properties of oyster shells were characterized. As such, crystallographic analysis presented that oyster shells had a hexagonal crystalline shape, and calcination process reduced their crystalline size, volume (grain dimension), and bond length, which strongly affected antimicrobial efficacy. Results showed that the main components of uncalcined and calcined oyster shells were CaCO3 and CaO, by which CaO was found to be the main antimicrobial component. Notably, calcined oyster shells showed antimicrobial potency against both Gram‐negative (Escherichia coli) and Gram‐positive (Staphylococcus aureus). Furthermore, cytotoxicity analysis proved that calcined oyster shells had good cell viability and low cytotoxicity. Results highlighted that calcined oyster shells, particularly those treated at 750°C, could be a biocompatible alternative to synthetic biocidal and antimicrobial agents using in food packaging, biomedical, and cosmetic industries.

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Section: Resultsmentioning

confidence: 99%

Effects of calcination temperature on morphological and crystallographic properties of oyster shell as biocidal agent

Park

Sadeghi

Thanakkasaranee

et al. 2020

Int J Applied Ceramic Tech

View full text Add to dashboard Cite

show abstract

“…The conversion of MTT into purple-colored formazan by viable cells would be the indicator, with the percentage cell viability measured with microplate reader at 570 nm and 630 nm. The lighter the purple color in the results after adding the MTT reagent following incubation for 4 h, the more cells were alive after the treatment [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Development of In-Situ Spray for Local Delivery of Antibacterial Drug for Hidradenitis Suppurativa: Investigation of Alternative Formulation

et al. 2021

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Hidradenitis suppurativa (HS) has been considered an orphan disease with limited treatments available. The available topical treatment for this condition is clindamycin lotion; however, short retention and frequent application are the main setbacks. Thus, the present study aimed to attain an optimized antibacterial in situ spray formulation for the hidradenitis suppurativa skin condition, which gels once in contact with the skin surface at around 37 °C and possesses bioadhesion as well as sustained-release properties of the incorporated drug. Different concentrations of thermo-reversible gelling polymer, Pluronic F-127, were investigated along with the selected bioadhesive polymers, HPMC and SA. The optimized formulation F3 consisting of 18% Pluronic F-127 with 0.2% HPMC and 0.2% SA was characterized based on various physicochemical properties. The gelation temperature of F3 was found to be 29.0 ± 0.50 °C with a gelation time of 1.35 ± 0.40 min and a pH of 5.8. F3 had the viscosity of 178.50 ± 5.50 cP at 25 °C and 7800 ± 200 cP at 37 °C as the gel set. The optimized formulation was found to be bioadhesive and cytocompatible. Cumulative drug release was 65.05% within the time-frame of 8 h; the release pattern of the drug followed zero-order kinetics with the Higuchi release mechanism. The average zone of inhibition was found to be 43.44 ± 1.34 mm. The properties of F3 formulation reflect to improve residence time at the site of application and can enhance sustained drug release. Therefore, it could be concluded that optimized formulation has better retention and enhanced antimicrobial activity for superior efficacy against HS.

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“…In several previous studies, the antibacterial activity of DOXY has been reported to be enhanced by encapsulation in PNPs [ 44 ]. However, similar effect on DOXY’s antitumor activity has not been tested before.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Antitumor Activity of Doxycycline Polymeric Nanoparticles: Effect on Tumor Apoptosis in Solid Ehrlich Carcinoma

et al. 2020

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Objectives: The aim of this study was to prepare doxycycline polymeric nanoparticles (DOXY-PNPs) with hope to enhance its chemotherapeutic potential against solid Ehrlich carcinoma (SEC). Methods: Three DOXY-PNPs were formulated by nanoprecipitation method using hydroxypropyl methyl cellulose (HPMC) as a polymer. The prepared DOXY-PNPs were evaluated for the encapsulation efficiency (EE%), the drug loading capacity, particle size, zeta potential (ZP) and the in-vitro release for selection of the best formulation. PNP number 3 was selected for further biological testing based on the best pharmaceutical characters. PNP3 (5 and 10 mg/kg) was evaluated for the antitumor potential against SEC grown in female mice by measuring the tumor mass as well as the expression and immunohistochemical staining for the apoptosis markers; caspase 3 and BAX. Results: The biological study documented the greatest reduction in tumor mass in mice treated with PNP3. Importantly, treatment with 5 mg/kg of DOXY-PNPs produced a similar chemotherapeutic effect to that produced by 10 mg/kg of free DOXY. Further, a significant elevation in mRNA expression and immunostaining for caspase 3 and BAX was detected in mice group treated with DOXY-PNPs. Conclusions: The DOXY-PNPs showed greater antitumor potential against SEC grown in mice and greater values for Spearman’s correlation coefficients were detected when correlation with tumor mass or apoptosis markers was examined; this is in comparison to free DOXY. Hence, DOXY-PNPs should be tested in other tumor types to further determine the utility of the current technique in preparing chemotherapeutic agents and enhancing their properties.

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In Vitro Evaluation of Antimicrobial Activity and Cytotoxicity of Different Nanobiotics Targeting Multidrug Resistant and Biofilm Forming Staphylococci

Cited by 35 publications

References 50 publications

Effects of calcination temperature on morphological and crystallographic properties of oyster shell as biocidal agent

Effects of calcination temperature on morphological and crystallographic properties of oyster shell as biocidal agent

Development of In-Situ Spray for Local Delivery of Antibacterial Drug for Hidradenitis Suppurativa: Investigation of Alternative Formulation

Synthesis and Antitumor Activity of Doxycycline Polymeric Nanoparticles: Effect on Tumor Apoptosis in Solid Ehrlich Carcinoma

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