In vitroelimination of onion yellow dwarf and shallot latent viruses in shallots (Allium cepavar.ascalonicumL.)

Fletcher, P. J.; Fletcher, J. D.; Lewthwaite, S. L.

doi:10.1080/01140671.1998.9514035

Cited by 18 publications

(6 citation statements)

References 8 publications

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“…In fact, the principal target of antiviral activity of these molecules is the inosine monophosphate dehydrogenase, an enzyme that catalyzes the conversion of inosine 5'-monophosphate (IMP) in xantosine 5'-monophosphate and is able to alter the pathway for the production of guanosine mono-di-and triphosphate. The presence of its inhibitor acts on this Weiland et al, 2004;Panattoni et al, 2006Panattoni et al, , 2007aSkiada et al, 2009bJames et al, 1997;Cieslinska, & Zawadzka, 1999;Cieslinska, 2002;O'Herlihy et al, 2003Janeckova, 1993Gabova, 1995;Chen & Li, 2001;Chen & Lu, 2000Kolbanova et al, 2004Iwanami & Ieki, 1994Cieslinska, 2003Conrad, 1991Green et al, 1992;Park et al, 1994;Kim et al, 1996;Truskinov & Rogozina, 1997;Faccioli & Zoffoli, 1998;Horackova, 1998;Nascimento et al, 2003;Fang et al, 2005Loi et al, 1991Schulze & Kluge, 1994Kim et al, 1994Xu & Niimi, 1999;Xu et al, 2000Ram et al, 2005Bertaccini et al, 2004Ramírez-Malagón et al, 2006Xu et al, 2004Lim et al, 1993;Toussaint et al, 1993;Fletcher et al, 1998;Freitas & Rezende, 1998;Yap et al, 1999;Fletcher & Fletcher, 200...…”

Section: Mechanism Of Actionmentioning

confidence: 99%

Review. Elimination of viruses in plants: twenty years of progress

Panattoni

Luvisi

Triolo

2013

Span. j. agric. res.

144

112

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To shed light on trends about elimination of viruses from plants, a bibliographic research was conducted to identify thermotherapy, chemotherapy and tissue culture trials published from 1991 through 2010. Among woody plants, grapevine, apple and peach are the most frequent targets of sanitation protocols because their health status is strictly regulated. Even if thermotherapy represents the preferred method for the host, grapevine viruses can also be eliminated with chemotherapy and tissue culture; apple viruses respond to chemotherapy as well. Although a similar trend was reported among herbaceous plants, chemotherapy was the most frequently used technique in potato. With regard to virus, thermotherapy was successfully applied against viruses belonging to 13 families and an unassigned genus. Instead, chemotherapy and tissue culture techniques eradicated viruses belonging to fewer families (nine). An interpretation of thermotherapy effects considers the new metabolic “pathways” triggered by the natural antiviral response emitted by the infected plant, with particular reference to virus-induced gene silencing. With regard to chemotherapy, several groups of antiviral drugs belong to inosine monophosphate dehydrogenase inhibitors, S-adenosylhomocysteine hydrolase inhibitors, neuraminidase inhibitors. Tissue culture, usually adopted to regenerate plantlets in biotechnological breeding programs, represents the less used tool for eliminate viruses from plants.

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Section: Mechanism Of Actionmentioning

confidence: 99%

Review. Elimination of viruses in plants: twenty years of progress

Panattoni

Luvisi

Triolo

2013

Span. j. agric. res.

144

112

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“…The use of virus-free propagating material has long been the most effective method for controlling viral diseases in vegetatively propagated plants (Conci, Perotto, Cafrune, & Lunello, 2005;Salomon, 2002). Meristem culture (Walkey, Webb, Bolland, & Miller, 1987), combining thermotherapy with meristem culture (Walkey et al, 1987;Sulistio, Sulistyaningsih, & Subandiyah, 2015), and combining chemotherapy with thermotherapy (Fletcher, Fletcher, & Lewthwaite, 1998) were reported to eradicate OYDV and SLV from shallot. Combining thermotherapy with meristem culture was also found to eradicate OYDV and SLV from garlic (Conci et al, 2005;Conci & Nome, 1991;Perotto, Conci, Cafrune, Alochis, & Bracamonte, 2003;Ramírez-Malagón, Pérez-Moreno, Borodanenko, Salinas-González, & Ochoa-Alejo, 2006).…”

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confidence: 99%

Combining thermotherapy with meristem culture for improved eradication of onion yellow dwarf virus and shallot latent virus from infected in vitro‐cultured shallot shoots

Wang

Hamborg

Blystad

et al. 2020

Annals of Applied Biology

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The present study described a combining thermotherapy with meristem culture for improved eradication of onion yellow dwarf virus (OYDV) and shallot latent virus (SLV) from co-infected in vitro-cultured shallot shoots. In vitro-cultured shoots infected with OYDV and SLV were thermo-treated at a constant temperature of 36 C for 0, 2 and 4 weeks, and then meristems (0.5 mm) containing 1-2 leaf primordia were excised and cultured for shoot regrowth. Meristem culture without thermotherapy produced much higher levels of survival (100%) and shoot regrowth (55%) than those (62% survival and 32% shoot regrowth) produced by the procedure combining 4 weeks of thermotherapy with meristem culture. However, much higher virus-free frequencies (70% for OYSV, 80% for SLV and 50% for both viruses) were obtained in the latter than those (10% for OYSV, 15% for SLV and 10% for both viruses) obtained in the former. Histological and subcellular studies showed that thermotherapy imposed stress or damage to the cells of meristems, thus resulting in reduced meristem survival and shoot regrowth. Studies on virus location revealed considerable alternations of virus distribution patterns in the thermo-treated meristems. The results of histological and subcellular studies and analysis of virus distribution pattern added valuable experimental data in the combining thermotherapy with meristem culture for virus eradication. These data provided explanations as to why combining thermotherapy with meristem culture improved the eradication of OYDV and SLV from the virus-infected in vitro shallot shoots.

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“…Plants that are propagated vegetative virus diseases are need to be solved [3,[5][6][7]. The way to eliminate it by chemotherapy treatment, with the added of antiviral Ribavirin in aseptic growing media The failure of meristematic tissue to grow and develop can be also caused by inaccuracies in explant selection or inappropriate composition of growth media.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Antiviral Ribavirin, Explant Size, Varieties on Growth and Development in Potato Meristematic

Karjadi

Gunaeni

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Potato (Solanum tuberosum L) is included in the genus Solanaceae, which were propagated by vegetative through tubers. Viral systemic disease is an important seed-borne disease, with tissue culture technique combined with explant size, added antiviral Ribavirin, could be used for eliminated diseases in growth and development. The aim of this study was to determine the effect of antiviral Ribavirin concentrations, explant size and varieties on growth and development of meristematic. The treatment was concentration antiviral Ribavirin (0, 15, 20 mg/l), in MS media, explants size (meristems, shoot tips), varieties (Granola, Medians, Atlantic). The research was conducted from March to September 2019 in the IVEGRI tissue culture laboratory. The results were concluded as follow 1). the percentage of explant shoot tip contamination were higher than the meristem. 2) the average number of shoots, nodes and roots per plantlet from the treatment of varieties, explant size and the added of antiviral Ribavirin has an effect, where the average number of shoot tips were higher than the meristem. 3) The percentage of plantlets abnormal up to 12 WAP was 20 to 50%. 4) The results of the serology DAS ELISA test for both varieties, the added of antiviral Ribavirin and explant meristem has been decreased the number of PLRV infected.

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In vitroelimination of onion yellow dwarf and shallot latent viruses in shallots (Allium cepavar.ascalonicumL.)

Cited by 18 publications

References 8 publications

Review. Elimination of viruses in plants: twenty years of progress

Review. Elimination of viruses in plants: twenty years of progress

Combining thermotherapy with meristem culture for improved eradication of onion yellow dwarf virus and shallot latent virus from infected in vitro‐cultured shallot shoots

The Effect of Antiviral Ribavirin, Explant Size, Varieties on Growth and Development in Potato Meristematic

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