In VitroEffects of Anti-Glaucomatous Eye Drops on Platelet-Activating Factor and its Metabolism

Abstract

This is the first study examine the relationship between PAF activity and glaucoma medication. Potency in PAF inhibition may be related to drop efficacy.

Anti-platelet effects of anti-glaucomatous eye drops: an in vitro study on human platelets

“…Our previous study determined the anti-aggregating effects of anti-glaucomatous drugs on washed rabbit platelets, and after stimulation by PAF only 17. Our results indicate that anti-glaucomatous drugs exhibit anti-platelet properties.…”

“…For the experiment, platelet-rich plasma (PRP) was isolated from healthy volunteers’ blood, and aggregation was induced in vitro using four known aggregating factors (platelet activating factor [PAF], adenosine diphosphate [ADP], thrombin receptor-activating peptide [TRAP], and arachidonic acid [AA]). In our previous study, we examined the inhibitory effects of a series of anti-glaucomatous eye drops on PAF-induced aggregation in washed rabbit platelets 17. To our knowledge, the current study is the first to investigate this effect on human platelets, and the first to test ADP, TRAP, and AA, as well.…”

Impact of prostaglandin glaucoma drops on platelet-activating factor action: an in vitro study

“…The sample was centrifuged at 630× g for 13 min at 25°C, and the supernatant was subsequently centrifuged at 1,400× g for 20 min at 25°C in order to obtain plasma (rich in platelets). The isolation of plasma and leukocytes from blood samples and the biological assay on washed rabbit platelets were carried out according to the methods described by Moschos et al16–19 The leukocytes were isolated after the sedimentation of the erythrocytes using 3.4 mL of dextran solution (3% dextran in 0.15 M NaCl) for 1 h at room temperature. The homogenates of leukocytes were aliquoted and stored at −80°C after protein determination by using Bradford method.…”

“…The extraction, purification, and determination of PAF were transacted according to the methods described previously 19–21. PAF was extracted according to the Bligh–Dyer method and was separated by a thin-layer chromatography (TLC) on silica gel G-coated plates with a development system consisting of chloroform/methanol/acetic acid/water (100:57:16:8, v/v/v/v).…”