<i>In vitro</i> dipeptidyl peptidase IV inhibitory activity and <i>in situ</i> insulinotropic activity of milk and egg white protein digests

Santos-Hernández, Marta; Cermeño, Maria; Recio, Isidra; Fitzgerald, Richard J.

doi:10.1039/d1fo00641j

Cited by 9 publications

(3 citation statements)

References 33 publications

(56 reference statements)

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“…This is in keeping with the results obtained by Nongonierma et al, which evidenced the positive impact of the SGID on the DPP-IV inhibitory activity of a whey protein hydrolysate, for which the IC 50 measured in vitro after SGID was closed (1.02 ± 0.05 mg.mL -1 ) of the value obtained here for dWPH (1.28 ± 0.38 mg.mL -1 ) (44). The IC 50 values obtained here for the digested whey protein isolate (1.36 ± 0.55 mg.mL -1 ) with the intestinal cell-based in situ method are comparables with those we obtained (1.64 ± 0.11 mg mL −1 ) for dWPI in a precedent study and slightly higher than those obtained by Santos-Hernández et al (1.17 ± 0.09 mg mL −1 ) for a digested whey protein concentrate (45).…”

Section: Food and Function Accepted Manuscriptsupporting

confidence: 90%

“…44 The IC 50 values obtained here for the digested whey protein isolate (1.36 ± 0.55 mg mL −1 ) with the intestinal cell-based in situ method are comparable with those we obtained (1.64 ± 0.11 mg mL −1 ) for dWPI in a precedent study and slightly higher than those obtained by Santos-Hernández et al (1.17 ± 0.09 mg mL −1 ) for a digested whey protein concentrate. 45…”

Section: Discussionmentioning

confidence: 99%

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In vitro comparison of whey protein isolate and hydrolysate for their effect on glucose homeostasis markers

et al. 2023

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Section: Food and Function Accepted Manuscriptsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

In vitro comparison of whey protein isolate and hydrolysate for their effect on glucose homeostasis markers

et al. 2023

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“…In comparison, Caron et al [ 42 ] found an IC 50 for hemoglobin protein (1.6 mg·mL −1 ), which is relatively close to the IC 50 found in this study (2.8 ± 0.4 mg·mL −1 ). Similarly, Theysgeur et al [ 33 ] reported an IC 50 of 3.7 mg·mL −1 for a hydrolyzed fish by-product, while Santos-Hernández et al [ 50 ] found an IC 50 value of 1.17 mg·mL −1 for whey protein digests, 0.87 mg·mL −1 for casein digests, and 1.75 mg·mL −1 for egg white digests using the same cell-based method. Rivero-Pino et al [ 27 ] found an IC 50 of 2.93 mg·mL −1 for pea protein using an in vitro model.…”

Section: Discussionmentioning

confidence: 96%

In Vivo and In Vitro Comparison of the DPP-IV Inhibitory Potential of Food Proteins from Different Origins after Gastrointestinal Digestion

Fleury

Deracinois

Dugardin

et al. 2022

IJMS

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Dipeptidyl-peptidase IV (DPP-IV) plays an essential role in glucose metabolism by inactivating incretins. In this context, food-protein-derived DPP-IV inhibitors are promising glycemic regulators which may act by preventing the onset of type 2 diabetes in personalized nutrition. In this study, the DPP-IV-inhibitory potential of seven proteins from diverse origins was compared for the first time in vitro and in vivo in rat plasma after the intestinal barrier (IB) passage of the indigested proteins. The DPP-IV-inhibitory potentials of bovine hemoglobin, caseins, chicken ovalbumin, fish gelatin, and pea proteins were determined in rat plasma thirty minutes after oral administration. In parallel, these proteins, together with bovine whey and gluten proteins, were digested using the harmonized INFOGEST protocol adapted for proteins. The DPP-IV half-maximal inhibitory concentration (IC50) was determined in situ using Caco-2 cells. The DPP-IV-inhibitory activity was also measured after IB passage using a Caco2/HT29-MTX mixed-cell model. The peptide profiles were analyzed using reversed-phase high-performance liquid chromatography tandem mass spectrometry (RP-HPLC-MS/MS) with MS data bioinformatics management, and the IC50 of the identified peptides was predicted in silico. The in vitro and in vivo DPP-IV-inhibitory activity of the proteins differed according to their origin. Vegetable proteins and hemoglobin yielded the highest DPP-IV-inhibitory activity in vivo. However, no correlation was found between the in vivo and in vitro results. This may be partially explained by the differences between the peptidome analysis and the in silico predictions, as well as the study complexity.

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Impact of growth conditions on the nitrogen, protein, colour and amino acid profiles of the cultured macroalga, Palmaria palmata

Idowu,

Amigo-Benavent,

Santos-Hernández

et al. 2023

J Appl Phycol

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The impact of growth conditions on the nitrogen, protein, colour and amino acid profiles of Palmaria palmata (from 2 locations) were investigated. Laboratory cultures of P. palmata were grown over a period of 18 days with and without nutrient addition, i.e., with culture medium (F/2) and urea (0.05 or 0.10 g L−1). The total nitrogen (TN) content of cultured P. palmata originating from longlines harvested from Cork increased with the addition of culture medium (F/2) and urea (0.05 g L−1) in comparison to the initial sample (4.15 ± 0.05 vs 1.73 ± 0.03%) while biomass from Galway increased with addition of culture medium (F/2) and urea (0.10 g L−1) in comparison to the initial sample (3.72 ± 0.05 vs 2.43 ± 0.06%). The protein nitrogen (PN) and non-protein nitrogen (NPN) of cultured P. palmata originating from Galway also increased when grown with the addition of nutrients. Nutrient enrichment during growth influenced the colour characteristics (lightness L*, redness a*, yellowness b* and colour difference ΔE) and the electrophoretic protein profile. Correlations between TN and ΔE (r = 0.945) for Cork originated sample, PN and ΔE (r =-0.944) and NPN and ΔE (r = 0.734) for Galway originated sample were observed. In-gel digestion followed by UPLC-MS/MS demonstrated that RuBiSCo, phycoerythrin and allophycocyanin expression in P. palmata was enhanced with nutrient addition. Amino acid profile and score analysis showed variation as a function of nutrient addition. Overall, nutrient inclusion in the growth medium provides a strategy for the enhanced production of high-quality protein by P. palmata.

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In vitro dipeptidyl peptidase IV inhibitory activity and in situ insulinotropic activity of milk and egg white protein digests

Abstract: Gastrointestinal digests of milk and egg white proteins inhibited DPP-IV activity. The absorbable fraction from the intestinal digests induced insulin secretion in BRIN-BD11 cells.

Cited by 9 publications

References 33 publications

In vitro comparison of whey protein isolate and hydrolysate for their effect on glucose homeostasis markers

In vitro comparison of whey protein isolate and hydrolysate for their effect on glucose homeostasis markers

In Vivo and In Vitro Comparison of the DPP-IV Inhibitory Potential of Food Proteins from Different Origins after Gastrointestinal Digestion

Impact of growth conditions on the nitrogen, protein, colour and amino acid profiles of the cultured macroalga, Palmaria palmata

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