<i>In Vitro</i> Differentiation of Osteocytes and Adipocytes from Human Placenta-Derived Cells

Sun, NZ; Ji, HS

doi:10.1177/147323001204000242

Cited by 8 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, according to our observations, the untreated SLCs expressed neural markers and could differentiate into neural lineage after a shorter induction period as compared with NTERA-2 cells. To investigate mesodermal differentiation of SCLs, an osteogenic medium, containing dexamethasone, ascorbate-2-phosphate and b-glycerol phosphate, was used based on several reports and our previous experience (Covas et al 2003;Ahmadian Kia et al 2011;Sun and Ji 2012). Differentiation of SLCs toward osteocytes was confirmed by their mineralized matrix stained with Alizarin Red S. RT-PCR studies also indicated the expression of Ocn and Opn in differentiated SLCs, quite similar to induced rMSCs.…”

Section: Discussionmentioning

confidence: 99%

Blastema cells derived from New Zealand white rabbit’s pinna carry stemness properties as shown by differentiation into insulin producing, neural, and osteogenic lineages representing three embryonic germ layers

et al. 2014

View full text Add to dashboard Cite

Stem cells (SCs) are known as undifferentiated cells with self-renewal and differentiation capacities. Regeneration is a phenomenon that occurs in a limited number of animals after injury, during which blastema tissue is formed. It has been hypothesized that upon injury, the dedifferentiation of surrounding tissues leads into the appearance of cells with SC characteristics. In present study, stem-like cells (SLCs) were obtained from regenerating tissue of New Zealand white rabbit's pinna and their stemness properties were examined by their capacity to differentiate toward insulin producing cells (IPCs), as well as neural and osteogenic lineages. Differentiation was induced by culture of SLCs in defined medium, and cell fates were monitored by specific staining, RT-PCR and flow cytometry assays. Our results revealed that dithizone positive cells, which represent IPCs, and islet-like structures appeared 1 week after induction of SLCs, and this observation was confirmed by the elevated expression of Ins, Pax6 and Glut4 at mRNA level. Furthermore, SLCs were able to express neural markers as early as 1 week after retinoic acid treatment. Finally, SLCs were able to differentiate into osteogenic lineage, as confirmed by Alizarin Red S staining and RT-PCR studies. In conclusion, SLCs, which could successfully differentiate into cells derived from all three germ layers, can be considered as a valuable model to study developmental biology and regenerative medicine.

show abstract

Section: Discussionmentioning

confidence: 99%

Blastema cells derived from New Zealand white rabbit’s pinna carry stemness properties as shown by differentiation into insulin producing, neural, and osteogenic lineages representing three embryonic germ layers

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Early studies expected the hPSCs to serve as building blocks of damaged tissues [ 182 , 187 , 188 , 189 , 190 , 191 , 192 , 193 ], but they seem to have limited differentiation potential [ 120 , 126 , 137 , 148 , 181 , 184 , 194 , 195 , 196 ]. The activity of hPSCs in allogeneic and xenogeneic administration seems to derive from their rich secretome, activated in response to a wide range of stress signals [ 114 , 130 , 180 , 197 , 198 , 199 , 200 , 201 , 202 , 203 ].…”

Section: Application Of Hpscs From Mature Placentae For Tissue Regeneration and Immune Modulationmentioning

confidence: 99%

Allogenic Use of Human Placenta-Derived Stromal Cells as a Highly Active Subtype of Mesenchymal Stromal Cells for Cell-Based Therapies

Gorodetsky

Aicher

2021

IJMS

View full text Add to dashboard Cite

The application of mesenchymal stromal cells (MSCs) from different sources, including bone marrow (BM, bmMSCs), adipose tissue (atMSCs), and human term placenta (hPSCs) has been proposed for various clinical purposes. Accumulated evidence suggests that the activity of the different MSCs is indirect and associated with paracrine release of pro-regenerative and anti-inflammatory factors. A major limitation of bmMSCs-based treatment for autologous application is the limited yield of cells harvested from BM and the invasiveness of the procedure. Similar effects of autologous and allogeneic MSCs isolated from various other tissues were reported. The easily available fresh human placenta seems to represent a preferred source for harvesting abundant numbers of human hPSCs for allogenic use. Cells derived from the neonate tissues of the placenta (f-hPSC) can undergo extended expansion with a low risk of senescence. The low expression of HLA class I and II on f-hPSCs reduces the risk of rejection in allogeneic or xenogeneic applications in normal immunocompetent hosts. The main advantage of hPSCs-based therapies seems to lie in the secretion of a wide range of pro-regenerative and anti-inflammatory factors. This renders hPSCs as a very competent cell for therapy in humans or animal models. This review summarizes the therapeutic potential of allogeneic applications of f-hPSCs, with reference to their indirect pro-regenerative and anti-inflammatory effects and discusses clinical feasibility studies.

show abstract

“…Numerous studies have demonstrated the differential potential and multilineage capacity of the placental derived stem cells through in vitro characterization and differentiation experiments (Sabapathy et al, 2012;Sun and ji, 2012;Susman et al, 2010;Chien et al, 2006). Recently, it has been reported that the placental derived stem cells differentiate into osteocytes, chondrocytes, adipocytes, hepatocytes and cardiomyocytes under specific culture conditions (Sabapathy et al, 2012;Sun and ji, 2012;Susman et al, 2010;Chien et al, 2006;Okamoto et al, 2007).We have selected differentiation pathways which are more important for the future program of stem cell transplantation at our institute -one is the use of cardiomyocytes in myocardial infarction and the another one is the use of osteocytes for bone disorders and bone tissue engineering (Sharma et al, 2013 (In press); Gupta et al, 2013 (In press); Potdar and S Kumar. 2013;Potdar and Prasanan, 2013).…”

Section: Expression Of Cardiomyocytes and Osteocytes Genes In Fetal Amentioning

confidence: 99%

“…All these markers are indications of specific phenotypes of stem cells. Differential potential of placental stem cells in to cardiomyocytes and osteocytes can be confirmed by expression of cardiac β heavy chain myosin, cardiac troponin and cardiac actin and ALP and OCN markers respectively (Sabapathy et al, 2012;Sun and ji, 2012;Susman et al, 2010;Chien et al, 2006;Okamoto et al, 2007). Few studies have shown an importance of some of the genes such as CD73, CD45, CD100, Vimentin and Ki67 in maintenance of human pregnancy.…”

Section: Introductionmentioning

confidence: 97%

Development and Molecular Characterization of Human Placental Mesenchymal Stem Cells from Human Aborted Fetal Tissue as a Model to Study Mechanism of Spontaneous Abortion

Potdar¹,

Chaugule²

2014

ASC

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have become an important component in stem cell therapies and can be isolated from various adult tissues. The human placenta is a fetomaternal organ having a high population of multipotent stem cells with better plasticity and differential potential. It is interesting to see the molecular phenotypes of these stem cells in placenta obtained from aborted fetus to understand the mechanism of spontaneous abortion. In the present study, we have proposed to isolate multipotent stem cells from human aborted placental tissue and characterized them by using molecular and morphological analysis. Our study has shown that the placenta derived Mesenchymal stem cells (hPMSCs) have elongated epithelioid like morphology and showed high pluripotency potential by expressing Oct4, Nanog and Sox2 along with expression of Mesenchymal, hematopoietic, cytokine and chemokine markers. We have further shown that hPMSCs cells have the capacity for spontaneous differentiation into cardiomyocytes and osteocytes which have confirmed the expression of specific cardiac and osteogenic markers. Interestingly, this study has clearly shown that there is an abnormal expression of CD 73, CD 100, CD 45, Ki67 and Vimentin in aborted fetal tissue thus indicates some role of these genes in the maintenance of pregnancies. Thus, this study concludes that hPMSCs cells can be used as an in vitro model system for the evaluation of the mechanism of spontaneous abortion. Similarly, due to their multipotent characteristic they can be used for future regenerative therapies for various disorders.

show abstract

In Vitro Differentiation of Osteocytes and Adipocytes from Human Placenta-Derived Cells

Abstract: Placenta-derived adherent cells can differentiate into either osteocytes or adipocytes in vitro. The human placenta may provide an alternative source of mesenchymal stem cells for basic research and clinical use.

Cited by 8 publications

References 25 publications

Blastema cells derived from New Zealand white rabbit’s pinna carry stemness properties as shown by differentiation into insulin producing, neural, and osteogenic lineages representing three embryonic germ layers

Blastema cells derived from New Zealand white rabbit’s pinna carry stemness properties as shown by differentiation into insulin producing, neural, and osteogenic lineages representing three embryonic germ layers

Allogenic Use of Human Placenta-Derived Stromal Cells as a Highly Active Subtype of Mesenchymal Stromal Cells for Cell-Based Therapies

Development and Molecular Characterization of Human Placental Mesenchymal Stem Cells from Human Aborted Fetal Tissue as a Model to Study Mechanism of Spontaneous Abortion

Contact Info

Product

Resources

About