<i>In Vitro</i> Cytotoxicity of Self-Adhesive Dual-Cured Resin Cement Polymerized Beneath Three Different Cusp Inclinations of Zirconia

Zhang, Chang-Yuan; Cheng, Yi-ling; Tong, Xin-Wen; Yu, Hao; Cheng, Hui

doi:10.1155/2019/7404038

Cited by 7 publications

(9 citation statements)

References 44 publications

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“…It should be noted that there was a significant reduction in cell viability in the TPP-10 group on day 7 compared with the negative control. However, the cell viability of the TPP-10 group at day 7 was nearly 75% relative to the negative control, which means that it was acceptable for cell proliferation according to the biological Evaluation Standards of Medical Devices of China (GB/T16886.5.2003). , All these results demonstrated that telmisartan-loaded PCL/PVP scaffolds had good biocompatibility and were feasible for further functional evaluation.…”

Section: Resultsmentioning

confidence: 81%

Immunomodulation of Telmisartan-Loaded PCL/PVP Scaffolds on Macrophages Promotes Endogenous Bone Regeneration

et al. 2022

ACS Appl. Mater. Interfaces

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Biomaterial–immune system interactions play an important role in postimplantation osseointegration to retain the functionality of healthy and intact bones. Therefore, appropriate osteoimmunomodulation of implants has been considered and validated as an efficient strategy to alleviate inflammation and enhance new bone formation. Here, we fabricated a nanostructured PCL/PVP (polycaprolactone/polyvinylpyrrolidone) electrospinning scaffold for cell adhesion, tissue ingrowth, and bone defect padding. In addition, telmisartan, an angiotensin 2 receptor blocker with distinct immune bioactivity, was doped into PCL-/PVP-electrospun scaffolds at different proportions [1% (TPP-1), 5% (TPP-5), and 10% (TPP-10)] to investigate its immunomodulatory effects and osteoinductivity/conductivity. Telmisartan-loaded scaffolds displayed in vitro anti-inflammatory bioactivity on lipopolysaccharide-induced M1 macrophages by polarizing them to an M2-like phenotype and exhibited pro-osteogenic properties on bone marrow-derived mesenchymal stem cells (BMSCs). Histological analysis and micro-CT results of a rat skull defect model also showed that the telmisartan-loaded scaffolds induced a higher M2/M1 ratio, less inflammatory infiltration, and better bone regenerative patterns. Furthermore, activation of the BMP2 (bone morphogenetic protein-2)-Smad signaling pathway was found to be dominant in telmisartan-loaded scaffold-mediated macrophage–BMSC interactions. These findings indicate that telmisartan incorporation with PCL/PVP nanofibrous scaffolds is a potential therapeutic strategy for promoting bone healing by modulating M1 macrophages to a more M2 phenotype at early stages of postimplantation.

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Section: Resultsmentioning

confidence: 81%

Immunomodulation of Telmisartan-Loaded PCL/PVP Scaffolds on Macrophages Promotes Endogenous Bone Regeneration

et al. 2022

ACS Appl. Mater. Interfaces

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“…It contains WST‐8, a water‐soluble tetrazolium salt, which, in viable cells, is reduced to a colored formazan dye by dehydrogenase activity (Ishiyama et al, 1997 ). It represents a well‐established and reproducible method for the quantification of cell viability and cell proliferation (Camassa et al, 2021 ; Schubert et al, 2019 ; Zhang et al, 2019 ; Zhu et al, 2019 ), and it is more sensitive than other colorimetric assays (Failli et al, 2013 ; Miyamoto et al, 2002 ).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of 3D‐printed, milled, and conventional oral splint resins to L929 cells and human gingival fibroblasts

Bürgers

Schubert

Müller

et al. 2022

Clinical & Exp Dental Res

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Objectives: Evidence on the biocompatibility of three-dimensional (3D)-printed and milled resins for oral splints is limited. This in vitro study assessed the influence of the manufacturing method on the cytotoxicity of oral splint resins on L929 cells and human gingival fibroblasts (GF1).Materials and Methods: Standardized specimens of four 3D-printed, two-milled, one-thermoformed, and one-pressed splint resin were incubated with L929 and GF1 cells for 24 h. Immunofluorescence and WST-8 assay were performed to evaluate cytotoxic effects. One-way analysis of variance and Tukey's multiple comparison test were applied with the variables "splint resin" and "manufacturing method" (p < .05).Results: Immunofluorescence showed attachment of L929 and GF1 cells to the splint resins. Irrespective of the manufacturing method, the WST-8 assay revealed significant differences between splint resins for the viability of L929 and GF1 cells.L929 cells generally showed lower viability rates than GF1 cells. The evaluation of cell viability by the manufacturing method showed no significant differences between 3D printing, milling, and conventional methods. Conclusions:The cytotoxic effects of 3D-printed, milled, and conventional oral splint resins were similar, indicating minor influence of the manufacturing method on biocompatibility. Cytotoxicity of the resins was below a critical threshold in GF1 cells. The chemical composition might be more crucial than the manufacturing method for the biocompatibility of splint resins.

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“…The remaining 29 articles [1][2][3][4][5][6][7][8][9][10]14,22,[25][26][27][28]31,[33][34][35][36][37][38][39][40][41][42][43][44] were selected for the quality analysis. Manual research also retrieved three studies [30,45,46], three randomized clinical trials [24,47,48], six reviews [15,23,29,[49][50][51], and three meta-analyses [11,12,20], which were used to broaden the introduction and discussion sessions. The selection process agreed with the PRISMA Statement, as shown in Figure 1.…”

Section: General Aspectsmentioning

confidence: 99%

“…Despite being more straightforward, professionals must know that problems can occur during cementation with SARC. Lack of polymerization efficiency, with the potential release of unreacted cytotoxic and genotoxic monomers [30], induces expansion of the cement layer, with polymerization shrinkage strain and high stresses caused by hygroscopic expansion, with possible crack formation and restoration failure [26,28]. An evenly distributed cement layer with low internal gap values is essential for correct seating and better mechanical properties, but also for a low-space volume of the cement and porosities inside the luting agent [5].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, differences in humidity, pH, and oral cavity temperature cause changes in dental materials [31]. SARCs exhibit good biocompatibility and marginal integrity, low microleakage [6], mechanical quality, and esthetic properties, being the most commonly used cements for the bonding of a restoration [30]. The adhesive strength of CAD-CAM ceramics to tooth substrates also depends on the type of ceramic, resin-matrix cement, the functional monomer used, and patient-related factors such as dentin thickness, occlusal loading, dental age, and proper oral hygiene [27].…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of Self-Adhesive Resin Luting Cement in CAD-CAM Blocks—A Systematic Review and Meta-Analysis

et al. 2023

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Self-adhesive resin cements (SARCs) are used because of their mechanical properties, ease of cementation protocols, and lack of requirements for acid conditioning or adhesive systems. SARCs are generally dual-cured, photoactivated, and self-cured, with a slight increase in acidic pH, allowing self-adhesiveness and increasing resistance to hydrolysis. This systematic review assessed the adhesive strength of SARC systems luted to different substrates and computer-aided design and manufacturing (CAD/CAM) ceramic blocks. The PubMed/MedLine and Science Direct databases were searched using the Boolean formula [((dental or tooth) AND (self-adhesive) AND (luting or cement) AND CAD-CAM) NOT (endodontics or implants)]. Of the 199 articles obtained, 31 were selected for the quality assessment. Lava Ultimate (resin matrix filled with nanoceramic) and Vita Enamic (polymer-infiltrated ceramic) blocks were the most tested. Rely X Unicem 2 was the most tested resin cement, followed by Rely X Unicem > Ultimate > U200, and μTBS was the test most used. The meta-analysis confirmed the substrate-dependent adhesive strength of SARCs, with significant differences between them and between SARCs and conventional resin-based adhesive cement (α < 0.05). SARCs are promising. However, one must be aware of the differences in the adhesive strengths. An appropriate combination of materials must be considered to improve the durability and stability of restorations.

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In Vitro Cytotoxicity of Self-Adhesive Dual-Cured Resin Cement Polymerized Beneath Three Different Cusp Inclinations of Zirconia

Cited by 7 publications

References 44 publications

Immunomodulation of Telmisartan-Loaded PCL/PVP Scaffolds on Macrophages Promotes Endogenous Bone Regeneration

Immunomodulation of Telmisartan-Loaded PCL/PVP Scaffolds on Macrophages Promotes Endogenous Bone Regeneration

Cytotoxicity of 3D‐printed, milled, and conventional oral splint resins to L929 cells and human gingival fibroblasts

Effectiveness of Self-Adhesive Resin Luting Cement in CAD-CAM Blocks—A Systematic Review and Meta-Analysis

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