In VitroCytokine Expression andIn VivoHealing and Inflammatory Response to a Collagen-Coated Synthetic Bone Filler

Abstract

The goal of the present work was to investigate the relationship between in vivo healing and inflammatory response and in vitro cytokine expression by macrophages of a synthetic bone filler (25% hydroxylapatite-75% β-tricalcium phosphate) bearing a surface nanolayer of collagen. A clinically accepted, state-of-the-art xenograft material was used as a “negative control,” that is, as a material that provides the correct clinical response for the intended use. In vitro data show that both materials exert a very low stimulation of proinflammatory cytokines by macrophages, and this was confirmed by the very mild inflammatory response detected in in vivo tests of local response in a rabbit model. Also, in vitro findings suggest a different mechanism of healing for the test and the control material, with a higher regenerative activity for the synthetic, resorbable filler, as confirmed by in vivo observation and literature reports. Thus, the simple in vitro model adopted provides a reasonable forecast of in vivo results, suggesting that new product development can be guided by in vitro tuning of cell-materials interactions.

Micro-Raman Spectroscopy of Dental Implants Subjected to Different Surface Treatments

“…From Brånemark's initial studies, titanium represents the most-used material, although not the only one, for the production of endosseous dental implants [1][2][3]. Success rates with titanium implants are high in both the short and long term; moreover, they are indicated in many particular situations [4][5][6][7][8][9][10].…”

Nanofeatured Titanium Surfaces for Dental Implantology: Biological Effects, Biocompatibility, and Safety

“…Similarly, in a human experimental study, results indicated that a significantly greater proportion of oral mucosa came into contact with the nanoporous TiO 2 surface than that with the unmodified surfaces [92]. This technique is also used to coat bone substitutes with a nanolayer of osteoconductive material [93].…”

Long-term results of autologous periosteum-derived micro-grafts with poly(lactic-go-glycolic acid) in sinus lift augmentation surgeries: A 7-years follow-up observational study

“…Nowadays, the demand for new and more efficient and performing materials to be applied in the dental field is growing [3] , [4] , [5] , [6] , [7] . The latest trends indicate that the use of a hybrid material, consisting of a scaffold and a biological component, is the future of dental bone tissue engineering [8] .…”