<i>In vitro</i> Cultures of <i>Bituminaria bituminosa:</i> Pterocarpan, Furanocoumarin and Isoflavone Production and Cytotoxic Activity Evaluation

D’Angiolillo, Francesca; Pistellia, Laura; Noccioli, Cecilia; Ruffoni, B.; Piaggi, Simona; Scarpato, Roberto; Pistelli, Luisa

doi:10.1177/1934578x1400900411

Cited by 9 publications

(21 citation statements)

References 28 publications

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“…During the vegetative stage, mycorrhizal plants contained significantly higher amounts of angelicin and psoralen compared with control plants, where these molecules were not detectable; such compounds represent the typical furanocoumarins in Fabaceae, in particular angelicin, which has been reported as the major component of furanocumarins in wild‐grown B. bituminosa leaves sampled in Elba Island (D'Angiolillo et al . ). During the flowering stage, AMF inoculation decreased the production of bitucarpin A and erybraidin C, the latter representing the major component of pterocarpans in wild‐grown B. bituminosa leaves (D'Angiolillo et al .…”

Section: Discussionmentioning

confidence: 97%

“…During the flowering stage, AMF inoculation decreased the production of bitucarpin A and erybraidin C, the latter representing the major component of pterocarpans in wild‐grown B. bituminosa leaves (D'Angiolillo et al . ). The only data available on the role of AMF in the development and growth of B. bituminosa concern its ability to survive in polluted sites, which increased when inoculated with AMF (Alguacil et al .…”

Section: Discussionmentioning

confidence: 97%

“…A modified extraction procedure was followed in this work to analyse specific phytochemicals and avoid any acidic treatment, to exclude the alteration of secondary metabolites present in the homogenous samples, according to D'Angiolillo et al . (). Leaves were extracted with chloroform (2 × 0.25 l, 1 week).…”

Section: Methodsmentioning

confidence: 97%

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Arbuscular mycorrhizal fungi alter the content and composition of secondary metabolites in Bituminaria bituminosa L.

et al. 2017

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Secondary metabolites may be affected by arbuscular mycorrhizal fungi (AMF), which are beneficial symbionts associated with the roots of most plant species. Bituminaria bituminosa (L.) C.H.Stirt is known as a source of several phytochemicals and therefore used in folk medicine as a vulnerary, cicatrising, disinfectant agent. Characteristic metabolites found in B. bituminosa are furanocoumarins and pterocarpans, which are used in cosmetics and as chemotherapeutic agents. Here we address the question whether AMF inoculation might affect positively the synthesis of these phytochemicals. B. bituminosa plants were inoculated with different AMF and several metabolites were assessed during full vegetative stage and flowering phase. Pigments (chlorophylls and carotenoids), polyphenols and flavonoids were spectrophotometrically determined; specific isoflavones (genistein), furanocoumarins (psoralene and angelicin), pterocarpans (bitucarpin A and erybraedin C) and plicatin B were assessed with HPLC; leaf volatile organic compounds were analysed using SPME and identified by GC-MS. During the vegetative stage, the inoculated plants had a high amount of furanocoumarins (angelicin and psoralen) and pterocarpans (erybraedin C and bitucarpin A). The analysis of volatile organic compounds of inoculated plants showed different chemical composition compared with non-mycorrhizal plants. Given the important potential role played by furanocoumarins and pterocarpans in the pharmaceutical industry, AMF inoculation of B. bituminosa plants may represent a suitable biotechnological tool to obtain higher amounts of such metabolites for pharmaceutical and medicinal purposes.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

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Arbuscular mycorrhizal fungi alter the content and composition of secondary metabolites in Bituminaria bituminosa L.

et al. 2017

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“…However, the antitumor effects of angelicin in human cervical carcinoma, as well as the mechanisms underlying its actions, are largely unknown. D'Anqiolillo et al (24) reported that angelicin exerts cytotoxic activity on HeLa cells, but did not elucidate the exact mechanism by which this occurs (24). Therefore, the aim of the present study was to assess the effects of angelicin on the human cervical carcinoma cell lines, HeLa and SiHa, and to investigate the molecular mechanisms underlying its action.…”

Section: Discussionmentioning

confidence: 99%

Angelicin inhibits the malignant behaviours of human cervical cancer potentially via inhibiting autophagy

Chen

Liang

et al. 2019

Exp Ther Med

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Angelicin is an active compound isolated from the Chinese herb Angelica archangelica, which has been reported to exert antitumor effects by inhibiting malignant behaviors in several types of tumor, including proliferation, colony formation, migration and invasion. However, the effects of angelicin on human cervical cancer cells is yet to be elucidated. The present study evaluated the antitumor effects of angelicin on cervical cancer cells. The results demonstrated that cervical cancer cells were more sensitive to angelicin than cervical epithelial cells. At its IC30, angelicin inhibited the proliferation of HeLa and SiHa cells by blocking the cell cycle at the G1/G0 phase and inhibiting other malignant behaviors, including colony formation, tumor formation in soft agar, migration and invasion. At the IC50, angelicin induced cell death potentially by promoting apoptosis. By identifying the hallmarks of autophagy, it was observed that angelicin treatment caused the accumulation of microtubule associated protein 1 light chain 3-β (LC3B) in the cytoplasm of HeLa and SiHa cells. Western blotting results demonstrated that cleaved LC3B-II and autophagy related proteins (Atg)3, Atg7 and Atg12-5 were upregulated following angelicin treatment. It was also determined that the phosphorylation of mTOR was induced by angelicin treatment. Furthermore, the inhibition of angelicin-induced mTOR phosphorylation did not disrupt its inhibitory effect on autophagy, indicating that angelicin inhibited autophagy in an mTOR-independent manner. Taken together, the present results suggested that angelicin regulated malignant behaviors in cervical cancer cells by inhibiting autophagy in an mTOR-independent manner. Findings suggested that autophagy might be a potential therapeutic target for cervical cancer.

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“…Further our observation of green spots on callus indicate the high callus growth rate as well callus ability to produce shoots. Researchers have succeeded in producing several valuable secondary phytochemicals in unorganized callus cultures [ 14 , 35 , 41 , 42 , 43 , 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Production of Echioidinin, 7-O-Methywogonin from Callus Cultures of Andrographis lineata and Their Cytotoxicity on Cancer Cells

et al. 2015

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Andrographis lineata is an herbal medicinal plant used in traditional medicine as a substitute for Andrographis paniculata. Here, using mature leaf explants of A. lineata we demonstrate for the first time the callus induction established on MS medium containing 1.0 mg l–1 IAA. Dried callus was subjected to solvent extraction with acetone. Further the acetone residue was separated by silica gel column chromatography, crystallized and characterized on the basis of nuclear magnetic resonance (proton and c13) and liquid chromatographic mass spectroscopy. This analysis revealed the occurrence of two known flavones namely, 7-O-methylwogonin (MW) and Echioidinin (ED). Furthermore, these compounds were tested for their cytotoxicity against leukemic cell line, CEM. We identify that ED and MW induced cytotoxicity in a time- and concentration-dependent manner. Further increase in the LDH release upon treatment with ED and MW further confirmed our cytotoxicity results against leukemic cell line. Strikingly, MW was more potent than ED when compared by trypan blue and MTT assays. Our results recapitulate the utility of callus cultures for the production of plant specific bioactive secondary metabolites instead of using wild plants. Together, our in vitro studies provide new insights of A. lineata callus cultures serving as a source for cancer chemotherapeutic agents.

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In vitro Cultures of Bituminaria bituminosa: Pterocarpan, Furanocoumarin and Isoflavone Production and Cytotoxic Activity Evaluation

Cited by 9 publications

References 28 publications

Arbuscular mycorrhizal fungi alter the content and composition of secondary metabolites in Bituminaria bituminosa L.

Arbuscular mycorrhizal fungi alter the content and composition of secondary metabolites in Bituminaria bituminosa L.

Angelicin inhibits the malignant behaviours of human cervical cancer potentially via inhibiting autophagy

In Vitro Production of Echioidinin, 7-O-Methywogonin from Callus Cultures of Andrographis lineata and Their Cytotoxicity on Cancer Cells

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