<i>In Vitro</i> Cultures for the Production of Secondary Metabolites

Scarpa, Grazia Maria; Prota, Vanda; Schianchi, Nicola; Manunta, Federica

doi:10.5772/intechopen.101880

Cited by 4 publications

(3 citation statements)

References 94 publications

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“…In vitro culture is an efficient alternative method for obtaining medicinally significant plant metabolites [ 31 ]. This study aimed to investigate the impact of in vitro-cultivated calluses of A. modesta on the production of secondary metabolites, volatile compounds, and antibacterial and antioxidant activities in comparison to the leaves of the parent plant.…”

Section: Resultsmentioning

confidence: 99%

Variation of antibacterial and antioxidant secondary metabolites and volatiles in leaf and callus extracts of Phulai (Acacia Modesta Wall.)

Hagaggi,

Abdul-Raouf,

Radwan

2024

BMC Plant Biol

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Background Acacia species are economically significant as medicinal plants that have been utilized since ancient times. Acacia modesta has been reported to possess potent antibacterial and antioxidant properties, but its growth rate is slow. In this study, we hypothesized that inducing callus in vitro from A. modesta could enhance the production of antibacterial and antioxidant secondary metabolites, thereby circumventing the issues of slow growth and excessive harvesting of the plant. Results The callus was induced from axillary buds on MS medium supplemented with 1 mg/L of 2,4-D and 1 mg/L of BAP. The secondary metabolites, volatile compounds, antibacterial activity, and antioxidant activity of the callus and parent plant leaf extracts were evaluated. The results revealed that the content of phenolics and flavonoids, the number of volatile compounds, and the antibacterial and antioxidant activities of the callus extract were significantly enhanced (P ≤ 0.05) compared to the leaf extract. The antibacterial and antioxidant effects were strongly correlated with the total phenolic and flavonoid content in the extracts. Conclusions Our findings suggest that in vitro callus culture increases the production of phenolics, flavonoids, and volatile compounds. This subsequently enhances the antibacterial and antioxidant properties of A. modesta.

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Section: Resultsmentioning

confidence: 99%

Variation of antibacterial and antioxidant secondary metabolites and volatiles in leaf and callus extracts of Phulai (Acacia Modesta Wall.)

Hagaggi,

Abdul-Raouf,

Radwan

2024

BMC Plant Biol

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“…The traditional production system where plants are collected from nature has also many disadvantages such as the inability to obtain metabolites in standard quantity and quality, scarcity of pure and standardized plant material, dependence on the season, and geographical restrictions. To avoid these disadvantages, in vitro secondary metabolite production (SMP) has been considered as an alternative method for obtaining valuable metabolites from plants (Scarpa et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…The main disadvantage of in vitro SMP is low productivity. Therefore, various approaches can be employed to increase low productivity, such as changing the nutrient medium and cultural conditions, applying growth regulators, and adding precursors or intermediate metabolites to the culture media (Scarpa et al, 2022). Elicitor applications, which stimulate the enzymes and genes involved in secondary metabolite synthesis, are also one of the methods to increase SMP under in vitro conditions.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of in vitro production of tropane alkaloids and phenolic compounds in Hyoscyamus niger by culture types and elicitor treatments

ALBAYRAK,

DEMIRCI,

BAYDAR

2023

Preprint

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This research aimed to identify the influences of 24-epibrasinolide (EBL) and methyl jasmonate (MJ) applications on growth parameters and secondary metabolite syntheses in adventitious root and cell suspension cultures of Hyoscyamine niger. Therefore, EBL at different concentrations (0.5, 1, and 2 mg L− 1), individually and together with 1 mM MJ were implemented in root and cell suspension cultures. In root cultures, the optimum concentration for achieving the highest root growth parameters was found to be 2 mg L− 1 of EBL. On the other hand, in cell suspension cultures, the best cell growth was observed when 1 mg L− 1 of EBL was applied. In root cultures, the highest scopolamine and hyoscyamine amounts were obtained when 1 mM MJ was applied in combination with EBL at concentrations of 1 and 2 mg L− 1, respectively. In cell cultures, 2 mg L− 1 EBL for scopolamine and 0.5 mg L− 1 EBL for hyoscyamine were found to be the best among all applications. Compared to the control, elicitor applications also stimulated phenolic production in both roots and cells, depending on their combinations and concentrations. Additionally, it was also found that the metabolite production performance of adventitious roots was higher than that of cells. In conclusion, it was suggested that the use of MJ and EBL could be a promising strategy for enhancing the accumulation of scopolamine, hyoscyamine, and phenolic substances in root and cell cultures of H. niger.

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Enhancement of in vitro production of tropane alkaloids and phenolic compounds in Hyoscyamus niger by culture types and elicitor treatments

Albayrak,

Demirci,

Baydar

2024

Plant Cell Tiss Organ Cult

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This study aimed to determine the effects of 24-epibrasinolide (EBL) and methyl jasmonate (MJ) treatments on growth parameters and secondary metabolite synthesis in adventitious root and cell suspension cultures of Hyoscyamus niger. Therefore, different concentrations (0.5, 1 and 2 mg L−1) of EBL alone and combined with 224.3 mg L−1 (1 mM) MJ were applied to root and cell suspension cultures. 2 mg L−1 and 1 mg L−1 EBL were determined as the treatments in which the highest values were obtained in terms of growth criteria in root and cell cultures, respectively. In root cultures, the highest scopolamine accumulation (2.57 mg g−1) was obtained from the combination of 2 mg L−1 EBL and MJ, while the highest value (0.66 mg g−1) for hyoscyamine was observed in the roots treated with 1 mg L−1 EBL and MJ. In cell cultures, 2 mg L−1 EBL for scopolamine and 0.5 mg L−1 EBL for hyoscyamine were found to be the best applications and calculated as 0.51 µg g−1 and 0.28 µg g−1, respectively. EBL and MJ treatments also stimulated total phenolic content (TPC). The highest TPC in root cultures was detected as 18.01 mg g−1 with the combination of MJ while in cell cultures, maximum TPC was observed in cells applied with 2 mg L−1 EBL and MJ as 11.56 mg g−1. When EBL and MJ were applied to root and cell suspension cultures, significant changes occurred in the amount of phenolic compounds. Co-application of EBL and MJ significantly increased the amount of gallic acid, catechin, epicatechin, cinnamic acid and chlorogenic acid in root cultures. The application of 2 mg L−1 EBL was determined as the most suitable application for gallic acid, catechin, epicatechin, p-coumaric acid, and caffeic acid in cell cultures. It was also found that the metabolite production performance of adventitious roots was higher than that of cells. In conclusion, it was suggested that the use of MJ and EBL may be a promising strategy to enhance the accumulation of scopolamine, hyoscyamine and phenolics in root and cell cultures of H. niger.

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In Vitro Cultures for the Production of Secondary Metabolites

Cited by 4 publications

References 94 publications

Variation of antibacterial and antioxidant secondary metabolites and volatiles in leaf and callus extracts of Phulai (Acacia Modesta Wall.)

Variation of antibacterial and antioxidant secondary metabolites and volatiles in leaf and callus extracts of Phulai (Acacia Modesta Wall.)

Enhancement of in vitro production of tropane alkaloids and phenolic compounds in Hyoscyamus niger by culture types and elicitor treatments

Enhancement of in vitro production of tropane alkaloids and phenolic compounds in Hyoscyamus niger by culture types and elicitor treatments

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