<i>In vitro</i>culture of raspberry (<i>Rubus ideaus</i>) for mass propagation

Welander, Margareta

doi:10.1080/14620316.1985.11515656

Cited by 21 publications

(10 citation statements)

References 6 publications

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“…In this study we found that the most effective way to reduce the incidence of chlorosis was to reduce the light intensity under which cultures were maintained, and supplementing a third strength of micro-and macro elements MT solution with IBA and AC. The effectiveness of this procedure could be due to a combination of the IBA and AC reducing mineral toxicity in the cultures (Anderson 1980;Snir 1981;Welander 1985) and improving root primordium formation. Inclusion of AC in the culture medium reduced the light intensity at the cutting surface and may have improved rooting ability.…”

Section: Discussionmentioning

confidence: 97%

“…This is because of the rapid development of shoot and leaf chlorosis and subsequent dieback in vitro (Snir 1981) or difficulties in getting shoots efficiently elongated (Welander 1985). Consequently, significant effort has been put into overcoming this problem, including manipulation of the composition and strength of the culture medium (Anderson 1980;James et al 1980;Welander 1985;Zawadzka and Orlikowska 2006). In this study we found that the most effective way to reduce the incidence of chlorosis was to reduce the light intensity under which cultures were maintained, and supplementing a third strength of micro-and macro elements MT solution with IBA and AC.…”

Section: Discussionmentioning

confidence: 99%

“…Some Rubus species and cultivars are reported to be extremely difficult to culture, with low micropropagation rates. This is because of the rapid development of shoot and leaf chlorosis and subsequent dieback in vitro (Snir 1981) or difficulties in getting shoots efficiently elongated (Welander 1985). Consequently, significant effort has been put into overcoming this problem, including manipulation of the composition and strength of the culture medium (Anderson 1980;James et al 1980;Welander 1985;Zawadzka and Orlikowska 2006).…”

Section: Discussionmentioning

confidence: 99%

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Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus

Miller

Hall³

et al. 2009

Plant Cell Tiss Organ Cult

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Factors affecting micropropagation efficiency of 32 selections of Rubus, including the pre-treatment and initiation culture stages, were investigated. Chilling at 4°C for 6 weeks as a pre-treatment significantly promoted in vitro initiation culture; up to 65% of initiation cultures post chilling were successful. The cytokinin 6-benzyladenine (BA) was the most effective of the three tested for promotion of multiple shoot development in culture, with an average of three to seven shoots or plantlets developed from each single node. Alternating the concentration of BA between 4.44 and 13.31 lM from sub-culture to subculture improved recalcitrant Rubus micropropagation, and reduced the problems associated with long-term culture in the presence of high concentrations of BA. Reduction of the strength of macro-and micro-elements in the basal medium from half to one-third, with addition of 0.49 lM indole-3-butyric acid and 0.05% activated charcoal, and placing the cultures under reduced light intensity (17 lmol m -2 s -1 ), was found to alleviate chlorosis and improve micropropagation with high quality Rubus plantlets. Response to in vitro culture differed greatly and consequently different methods of micropropagation were required by different genotypes. From these selections, three types of micropropagation including micro-cutting, micro-shoots and multi-shoots were observed, and their efficiency was characterized.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus

Miller

Hall³

et al. 2009

Plant Cell Tiss Organ Cult

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show abstract

“…Welander (1985) reported that meristem growth of six raspberry genotypes improved if the concentration of NH4N03 and KN03 in MS medium was reduced to half strength, whereas rooting was enhanced by one fifth dilution of macroelements. Welander (1985) reported that meristem growth of six raspberry genotypes improved if the concentration of NH4N03 and KN03 in MS medium was reduced to half strength, whereas rooting was enhanced by one fifth dilution of macroelements.…”

Section: Culture Mediamentioning

confidence: 99%

Meristem and Shoot Tip Culture: Requirements and Applications

Nehra¹,

Kartha²

1994

Plant Cell and Tissue Culture

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“…That is why the method of clonal micropropagation (6,8,11,12,13,14) of a number of fruit cultures, including berry species was studied. Its practical application for propagation of some species became possible because of the higher multiplication coefficient compared to the traditional method of propagation.…”

Section: Introductionmentioning

confidence: 99%