<i>In vitro</i> characteristics of human fetal cells obtained from chorionic villus sampling and amniocentesis

Chang, Hsiao-Chen; Jones, Oliver W.

doi:10.1002/pd.1970080507

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…Secondary cultures. Because of the large numbers of cells needed for the experiments, primary cultures, established from 20-30 AF samples at 15-20 weeks' gestation, were subcultured by trypsin-EDTA (GIBCO,, and combined to grow in MEM-alpha medium supplemented with 20 per cent FBS, penicillin G (50 units/ml), streptomycin (50 pg/ml), fungizone (0.12 pg/ml), and glutamine (2 mM) in a 5 per cent CO, incubator at 37°C (Chang and Jones, 1985;1988). The same amount of single cell suspension (1 ml per plate) prepared from these subcultures was then seeded onto uncoated or coated (either by A F or by FBS) plates.…”

Section: Amniotic Fluid Cell Culturementioning

confidence: 99%

Enhancement of amniocyte growth on a precoated surface

Chang

Jones

1991

Prenatal Diagnosis

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This study investigates whether cell-free amniotic fluid facilitates cell attachment to the surface of culture plates and thereby promotes rapid amniocyte growth. Isolated or pooled cell-free amniotic fluid samples at different volumes were added to culture plates. Trypsinized subcultures, grown in Eagle's minimum essential alpha medium supplemented with fetal bovine serum (4-20 per cent), were monitored by cell counts. The results demonstrated growth stimulation on culture plates precoated with amniotic fluid. The minimal time for coating the culture plates was 6 h. Maximal coating was observed after an overnight incubation with 2-3 ml of the fluid per culture vessel. No synergistic effect from addition of fetal bovine serum to amniotic fluid was observed. A freshly coated surface provided the best amniocyte growth. When primary cultures are grown on a precoated surface, there is an increase in colony counts in 80 per cent of the samples tested. This method may be used to improve amniocyte growth, especially in samples with relatively small numbers of cells.

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Section: Amniotic Fluid Cell Culturementioning

confidence: 99%

Enhancement of amniocyte growth on a precoated surface

Chang

Jones

1991

Prenatal Diagnosis

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“…Redman et al (1984) showed a difference in Class I expression between extra-villous and villous trophoblast, the former being positive and the latter negative. These various data may be resolved by more refined methods to define, separate, and culture the cell types in chorionic villi (Niazi et al, 1981;Loke et al, 1986;Chang and Jones, 1988). Table 2 summarizes the characteristics of chorionic villus and amniotic fluid cultures as defined in this laboratory during the past 12 years.…”

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Differentiation in human chorionic villus cultures: hCG and hla expression

et al. 1989

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The present report describes methods to separate, culture, and study syncytio-cytotrophoblast and mesenchymal core of the first-trimester human chorionic villus. The cultured outer layer cells (syncytio-cytotrophoblast) are multinucleated, pleomorphic, and active in the formation of human chorionic gonadotrophin (hCG). The mesenchymal core cells are more fibroblast-like in appearance, do not show multinucleation, and have less hCG in their culture media. Both cultured cell types express HLA (ABC) Class I histocompatibility antigens but not HLA (DR) Class II antigens. These and previous studies from this laboratory postulate different embryonic origins: (1) Syncytio-cytotrophoblast cultures of chorionic villus derive from differentiated trophoblast and preserve multinucleation as well as hCG hormone function. (2) Cells cultured from the chorionic villus core originate from extraembryonic mesenchyme. (3) Amniocytes (AF cells) cultured from amniotic fluid resemble the multipotential and early-stage trophoblast, retaining pleomorphism, multinucleation, and lacunae formation as well as production of hCG, progesterone, oestrogen, basement membrane glycoprotein, and Type IV collagen. These cell types cultured from the chorionic villus and amniotic fluid provide a means for in vitro study of specific embryonic cell lineages.

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Amniotic Fluid Constituents, Cell Culture, and Neural Tube Defects

Dyke

Milunsky

2015

Genetic Disorders and the Fetus

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In vitro characteristics of human fetal cells obtained from chorionic villus sampling and amniocentesis

Cited by 6 publications

References 23 publications

Enhancement of amniocyte growth on a precoated surface

Enhancement of amniocyte growth on a precoated surface

Differentiation in human chorionic villus cultures: hCG and hla expression

Amniotic Fluid Constituents, Cell Culture, and Neural Tube Defects

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