In vitrobulblet regeneration from bulbscale explants ofLilium japonicumThunb. Effect of plant growth regulators and culture environment

In vitro regeneration and bulblet growth from lily bulbscale explants as affected by retardants, sucrose and irradiance

“…Incorporation of sucrose at various concentrations in the medium enhanced bulblet size. Sucrose at higher concentration was more effective in increasing average fresh mass and diameter of in vitro bulblets confirming the previous studies (Klerk et al 1992, Bonnier and Van Tuyl 1997, Marinengeli and Curvetto 1997. The increase in bulblet size in high concentration of sucrose was mainly due to an increase in starch and total carbohydrates (LangensGerrits et al 1997).…”

“…The difference was also observed in the growth habit of the bulblets, in dark, only fewer bulblets produced leaves. Maesato et al (1994) reported that the number of bulblets per explant was lower in dark. It could be expected that competition for nutrients among bulblets would be lower in dark (as only few bulblets were formed in dark) than in the bulblets formed in light.…”

“…Several attempts have been made to multiply lily through tissue culture. However, the bulblets raised through tissue culture, although have shown high multiplication rate remained small in size for commercial use (Niimi and Onozawa 1979, Tanaka et al 1991, Maesato et al 1994, Niimi 1995, Kumar et al 2001. In bulbous crops, vigour and growth of the plants are directly correlated with size of the underground organs.…”

“…Certain minimum size of the bulb is essential for the plant to flower. Plant growth regulators, sucrose as well as various environmental factors are known to play an important role on in vitro bulblet size and rate of multiplication in various bulbous crops (Kim and De Hertogh 1995, Maesato et al 1994, Lian et al 2003. In the present investigation an attempt has been made to study the effects of growth retardants, sucrose and irradiance on bulblet size and plant regeneration from bulbscale explants in oriental lily hybrid Star Gazer.…”

Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis.

“…Typically, in most of these protocols, combinations of auxins and cytokinins were used. Among cytokinins, BAP has been extensively used due to its pronounced effect on the adventitious bulblet formation (Takayama and Misawa, 1979;Maesato et al, 1994;Kędra and Bach, 2005). Rybczyński and Gomolińska (1989) described the influence of BAP at low concentration on bulblet formation in L. martagon.…”