In vitro biocontrol of Fusarium oxysporum with antagonistic microorganisms and In vivo effect on Solanum lycopersicum L.

Rubio-Tinajero, Sarahi; Osorio-Hernández, Eduardo; Drouaillet, Benigno Estrada; Silva-Espinosa, José Hugo Tomás; Martínez, Rafael; Espinoza-Ahumada, César Alejandro; Rios‐Velasco, Claudio; Torres-Castillo, Jorge Ariel

doi:10.1080/03601234.2022.2093590

Cited by 3 publications

(3 citation statements)

References 39 publications

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“…The role of T. asperellum as antagonistic against different plant fungi pathogens such as A. solani (Ronnie-Gakegne and Martínez-Coca, 2018), Fusarium oxysporum (Hao et al, 2022, Rubio-Tinajero et al, 2022Sharma et al, 2022), Sclerotinia sclerotiorum, A. solani, Phytophthora sphave (Sharma et al, 2022), Puccinia striiformis (Esmail et al, 2022) have been demonstrated. Alternaria comprise a group of species causing several damages on different plants, including apple trees, to which they can induce leaf blotch and fruit spot (Cabrefiga et al, 2023).…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

Application of Trichoderma asperellum in apple trees as a growth regulator and antagonist for the control of Alternaria sp.

MADRID-MOLINA

Pérez-Álvarez

Escobedo-Bonilla

et al. 2023

Not Bot Horti Agrobo

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Chihuahua state is the main apple producer in Mexico. The present study aimed to evaluate the effectiveness of Trichoderma asperellum as a plant growth regulator and antagonist against Alternaria sp. Three treatments were used: T1 = control, T2 = 200 mL of T. asperellum per tree; T3 = 100 mL of T. asperellum per tree. Agronomic variables were evaluated including number of leaves, shoots and flowers, disease incidence, and trunk thickness. Alternaria sp. was isolated from apple leaves at the experimental site in Guerrero County, Chihuahua, Mexico, and it was grown on solid PDA medium for morphological characterization. The molecular characterization was done by PCR using primers ITS1 and ITS4 producing products of 700 bp which were sequenced, submitted to GenBank (acc. no. OQ344593) and used for further phylogenetic analysis through Bayesian inference approach. Three clades were identified and the polytome topography recovered from clade 2 indicates a high genetic similarity with A. tenuissima (100% similarity according to BLAST). The analysis of T. asperellum as growth regulator only showed significant differences in trunk thickness and displayed higher values with T3 (p≤0.05). The presence of A. tenuissima was only observed in the control, which indicated the ability of Trichoderma to control the fungus. In this study T. asperellum was not an efficient plant growth regulator, but it was a good antagonist, and hence it can be recommended to control A. tenuissima. This is the first record of A. tenuissima in apple trees in Mexico. These results indicate that T. asperellum showed no benefit as plant growth regulator when applied to apple trees of the ‘Granny Smith’ variety.

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Section: Discussion Discussion Discussionmentioning

confidence: 99%

Application of Trichoderma asperellum in apple trees as a growth regulator and antagonist for the control of Alternaria sp.

MADRID-MOLINA

Pérez-Álvarez

Escobedo-Bonilla

et al. 2023

Not Bot Horti Agrobo

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“…Other research has reported higher values of fungal inhibition of encapsulated microorganisms. Rubio-Tinajero et al [23] evaluated the in vitro antagonistic effect of alginate microcapsules containing Trichoderma spp. and Bacillus spp., which inhibited Fusarium oxysporum by 84.7% and 83.7%, respectively.…”

Section: In Vitro Antifungal Activity Against the Mycelium Of Fungimentioning

confidence: 99%

“…In addition to maintaining viability, encapsulation has positive effects on the antifungal activity of the encapsulated bacteria [23,24]. We previously isolated two LAB strains, Lactiplantibacillus plantarum TEP15 and Lactiplantibacillus pentosus TEJ4, which inhibit the development of Colletotrichum gloeosporioides [25]; however, when applied in situ, these strains exhibit reduced antifungal activity.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Capacity of Microcapsules Containing Lactiplantibacillus plantarum TEP15 or Lactiplantibacillus pentosus TEJ4

Pérez-Ruiz,

Mejía-Reyes,

Rosas-Quijano

et al. 2024

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The use of lactic acid bacteria (LAB) for the biocontrol of fungi that cause fruit and vegetable deterioration is a highly promising strategy. However, one of the main challenges lies in maintaining both viability and antifungal activity even in conditions that are unfavorable for LAB. The microencapsulation of LAB can minimize the impact of environmental conditions, helping to maintain viability, but there is still little information on what occurs with respect to antifungal activity. Therefore, the objective of this study was to evaluate the effect of microencapsulation with several polymer blends on the viability and antifungal activity of Lactiplantibacillus plantarum TEP15 and L. pentosus TEJ4. Sodium alginate (2%), sodium alginate–gum arabic (2%:1%), sodium alginate–k-carrageenan (2%:0.05%), and sodium alginate–whey protein (0.75%:1.5%) were used as encapsulating polymers. After processing (day 0), as well as after 14 and 28 days of storage, the encapsulation efficiency, moisture content, bead size, and survival were evaluated. Likewise, the encapsulated bacteria were subjected to in vitro tests against Colletotrichum gloeosporioides, Penicillium AT21H10, and Fusarium sp. Capsules with sizes ranging from 1.47 mm to 1.88 mm were obtained, and all the wall materials tested had more than 85% encapsulation efficiency and allowed survival rates greater than 90% and 80% to be maintained after 14 and 28 days of storage, respectively. The encapsulated LAB inhibited the growth of mycelia by up to 100%, but, against spores, the greatest inhibition was 22.08% for all the fungi tested.

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In vitro biocontrol of phytopathogenic fungi isolated from the rhizosphere of multiple crops by a native Trichoderma strain

Santos Villegas,

Jiménez-Juárez,

Rosas Morales

et al. 2024

J Biol Res

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Phytopathogenic fungi associated with roots and leaves can cause significant losses in crops of commercial interest due to alterations in the growth and development of the host plants. In addition, they could contaminate fruits in the postharvest stage, provoking significant economic damage. In this regard, biocontrol by antagonistic fungi such as Trichoderma sp. (Peerson, 1974) has been shown as a viable eco-friendly solution. Accordingly, in this study, four genera of native phytopathogenic fungi, namely Fusarium (Link, 1809), Botrytis (Micheli & Peersoon, 1729), Alternaria (Nees, 1817), and Colletotrichum (Corda, 1831)], as well as of native postharvest fungi, namely Rhizopus (Anton de Bary, 1886), Mucor (Saccardo, 1887), Penicillium (Friedrich, 1809), and Aspergillus (Micheli, 1728), were isolated and identified from the rhizosphere of multiple crops of an unstudied autochthonous region in Puebla, Mexico. The isolated phytopathogens were tested in dual confrontation assays against a native Trichoderma strain with presumable antagonistic activity, finding a significant growth inhibition, reported for the first time. For the phytopathogenic fungi, the highest percentage of inhibition of radial growth (PIRG) was observed in Fusarium sp., followed by Alternaria sp., and Colletotrichum sp.; for the post-harvesting fungi, the best PIRG was found in Penicillium sp. (2), followed by Aspergillus sp., Rhizopus sp., Mucor sp., and Penicillium sp. (1).

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In vitro biocontrol of Fusarium oxysporum with antagonistic microorganisms and In vivo effect on Solanum lycopersicum L.

Cited by 3 publications

References 39 publications

Application of Trichoderma asperellum in apple trees as a growth regulator and antagonist for the control of Alternaria sp.

Application of Trichoderma asperellum in apple trees as a growth regulator and antagonist for the control of Alternaria sp.

Antifungal Capacity of Microcapsules Containing Lactiplantibacillus plantarum TEP15 or Lactiplantibacillus pentosus TEJ4

<i>In vitro</i> biocontrol of phytopathogenic fungi isolated from the rhizosphere of multiple crops by a native <i>Trichoderma</i> strain

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