In vitro biocompatibility evaluation of poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate) copolymer in fibroblast cells

Abstract

Among the various biomaterials available for tissue engineering and therapeutic applications, microbial polyhydroxyalkanoates offer the most diverse range of thermal and mechanical properties. In this study, the biocompatibility of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB); containing 50 mol % of 4-hydroxybutyrate] copolymer produced by Delftia acidovorans was evaluated. The cytotoxicity, mode of cell death, and genotoxicity of P(3HB-co-4HB) extract against V79 and L929 fibroblast cells were assessed using MTT assay, acridine orange/propidium iodide staining, and alkaline comet assay, respectively. Our results demonstrate that P(3HB-co-4HB) treated on both cell lines were comparable with clinically-used Polyglactin 910, where more than 60% of viable cells were observed following 72-h treatment at 200 mg/mL. Further morphological investigation on the mode of cell death showed an increase in apoptotic cells in a time-dependent manner in both cell lines. On the other hand, P(3HB-co-4HB) at 200 mg/mL showed no genotoxic effects as determined by alkaline comet assay following 72-h treatment. In conclusion, our study indicated that P(3HB-co-4HB) compounds showed good biocompatibility in fibroblast cells suggesting that it has potential to be used for future medical applications.

Amphiphilic PHA–mPEG copolymeric nanocontainers for drug delivery: Preparation, characterization and in vitro evaluation

“…7A). Our findings are in agreement with those of cells incubated with higher concentration of P(3HB-co-4HB) polymer showing the biocompatibility of this polymer to the cells (Siew et al, 2007). A similar effect was observed when fibroblast cells were incubated with P(3HB-co-33 mol% 3HV)-mPEG and P(3HB-co-20 mol% 4HB)-mPEG nanoparticles (Fig.…”

Influence of poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate‐co‐3‐hydroxyhexanoate) on growth and osteogenic differentiation of human bone marrow‐derived mesenchymal stem cells

“…As a family of naturally occurring polyesters, polyhydroxyalkanoates (PHAs) are produced by many microorganisms under unbalanced growth conditions 1–3. PHA polyesters have adjustable mechanical properties, biodegradability and good biocompatibility, and have attracted increasing interest as tissue engineering materials 4–7. Among PHA family, many studies were performed on poly(3‐hydroxybutyrate) (PHB) and poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) (PHBV) as biomaterials for biomedical applications 8–11.…”

Nanofibrous Scaffolds of Bio-Polyesters: In Vitro and In Vivo Characterizations and Tissue Response

“…From the histological observations, the biocompatibility of the P(3HB-co-97mol%-4HB) nanofibrous scaffold is by far the most superior among all of the electrospun scaffolds investigated in this current work. In addition, the in vitro toxicity of the P(3HB-co-4HB) biosynthesized from D. acidovorans which was determined by Siew and co-workers also demonstrated that this copolymer has good biocompatibility (Siew et al, 2007). Their recent genotoxicity study also revealed encouraging results for this PHA copolymer (Siew et al, 2008).…”