<i>In Vitro</i> Biocompatibility and Macrophage Uptake Assays of Poly(Urea-Urethane) Nanoparticles Obtained by Miniemulsion Polymerization

Valério, Alexsandra; Feuser, Paulo Emílio; Bubniak, Lorena dos Santos; Santos-Silva, Maria Cláudia; Araújo, Pedro Henrique Hermes de; Sayer, Cláudia

doi:10.1166/jnn.2017.13434

Cited by 6 publications

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“…DETC was encapsulated in beeswax nanoparticles with and without copaiba oil obtained by the double emulsion technique using pharmaceutically acceptable (non-toxic) lipids and surfactants. When the focus is the biomedical application the study of the physico-chemical properties (size, morphology and zeta potential) of nanoparticles is extremely important, since these can have biological implications, as in the cellular uptake, biological processes and biocompatibility [33][34][35][36].…”

Section: Resultsmentioning

confidence: 99%

Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro

Mazur

Feuser

Valério

et al. 2019

Colloids and Surfaces B: Biointerfaces

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Leishmaniasis is considered a neglected tropical disease that represents a Public Health problem due to its high incidence. In the search of new alternatives for Leishmaniasis treatment diethyldithiocarbamate (DETC) has shown an excellent leishmanicidal activity and the incorporation into drug carrier systems, such as solid lipid nanoparticles (SLNs), is very promising. In the present work DETC loaded in beeswax nanoparticles containing copaiba oil were obtained by the double emulsion/melt technique. The nanoparticles were characterized and leishmanicidal activity against L. amazonensis promastigotes forms and cytotoxicity in murine macrophages were evaluated. SLNs presented size below 200 nm, spherical morphology, negative charge surface, high encapsulation efficiency, above 80%, and excellent stability. Moreover, Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) analyses were performed to evaluate the chemical structure and possible interactions between DETC and SLNs. SLNs provided a protection for DETC, decreasing its cytotoxic effects in macrophages, which led to an improvement in the selectivity against the parasites, which almost doubled from free DETC (11.4) to DETC incorporated in SLNs (18.2). These results demonstrated that SLNs had a direct effect on L. amazonensis promastigotes without affect the viability of macrophage cell, can be a promising alternative therapy for the cutaneous treatment of L. amazonensis

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Section: Resultsmentioning

confidence: 99%

Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro

Mazur

Feuser

Valério

et al. 2019

Colloids and Surfaces B: Biointerfaces

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“…Furthermore, the hydrolyzability can be regulated by changing the units of polyureas [5]. Due to their excellent hydrolyzability and biocompatibility, polyureas have been developed for biomedical applications, including drug delivery [6,7,8].…”

Section: Introductionmentioning

confidence: 99%

PEGylated Polyurea Bearing Hindered Urea Bond for Drug Delivery

Chen

Wang

et al. 2019

Molecules

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In recent years, polyureas with dynamic hindered urea bonds (HUBs), a class of promising biomedical polymers, have attracted wide attention as a result of their controlled hydrolytic properties. The effect of the chemical structures on the properties of polyureas and their assemblies has rarely been reported. In this study, four kinds of polyureas with different chemical groups have been synthesized, and the polyureas from cyclohexyl diisocyanate and tert-butyl diamine showed the fastest hydrolytic rate. The amphiphilic polyurea composed of hydrophobic cyclohexyl-tert-butyl polyurea and hydrophilic poly(ethylene glycol) (PEG) was synthesized for the controlled delivery of the antitumor drug paclitaxel (PTX). The PTX-loaded PEGylated polyurea micelle more effectively entered into the murine breast cancer 4T1 cells and inhibited the corresponding tumor growth in vitro and in vivo. Therefore, the PEGylated polyurea with adjustable degradation might be a promising polymer matrix for drug delivery.

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“…For this kind of application, gold, magnetic, or cerium oxide nanoparticles, carbon nanotubes, graphene oxide, or even polymers may be conjugated to the aptamers (Sharma et al 2015). These nanostructured supports have been commonly synthesized and applied (Almeida et al 2017;Valério et al 2017;Chiaradia et al 2018;Hoelscher et al 2018;Maass et al 2019).…”

Section: Aptamers Conjugationmentioning

confidence: 99%

An overview and future prospects on aptamers for food safety

Schmitz

Valério

Oliveira

et al. 2020

Appl Microbiol Biotechnol

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Introduction Many bacteria are responsible for infections in humans and plants, being found in vegetables, water, and medical devices. Most bacterial detection methods are time-consuming and take days to give the result. Aptamers are a promising alternative for a quick and reliable measurement technique to detect bacteria present in food products. Selected aptamers are DNA or RNA oligonucleotides that can bind with bacteria or other molecules with affinity and specificity for the target cells by the SELEX or cell-SELEX technique. This method is based on some rounds to remove the non-ligand oligonucleotides, leaving the aptamers specific to bind to the selected bacteria. Compared with conventional methodologies, the detection approach using aptamers is a rapid, low-cost form of analysis. Objective This review summarizes obtention methods and applications of aptamers in the food industry and biotechnology. Besides, different techniques with aptamers are presented, which enable more effective target detection. Conclusion Applications of aptamers as biosensors, or the association of aptamers with nanomaterials, may be employed in analyses by colorimetric, fluorescence, or electrical devices. Additionally, more efficient ways of sample preparation are presented, which can support food safety to provide human health, with a low-cost method for contaminant detection. Key points • Aptamers are promising for detecting contaminants outbreaks. • Studies are needed to identify aptamers for different targets.

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In Vitro Biocompatibility and Macrophage Uptake Assays of Poly(Urea-Urethane) Nanoparticles Obtained by Miniemulsion Polymerization

Cited by 6 publications

References 0 publications

Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro

Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro

PEGylated Polyurea Bearing Hindered Urea Bond for Drug Delivery

An overview and future prospects on aptamers for food safety

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