In vitroassessment of the anthelmintic activity ofHedysarum carnosumDesf. at different phenological stages and from six locations in Tunisia

Abstract

Gastrointestinal nematodes are compromising productivity of grazing sheep and goats. Therefore, scientists have been looking for cost-effective alternative options. Forage legumes (Fabacea Family) contain tannins that could improve livestock performance and their health as well. The present study aimed to (i) determine the in vitro anthelmintic (AH) activity of 19 acetonic extracts of Hedysarum carnosum Desf on Haemonchus contortus by a larval exsheathment assay (LEA); (ii) test the anthelmintic activity of condensed tannins using a deactivating reagent, polyvinylpolypyrrolidone (PVPP); (iii) study the effect of location and the phenological stage on the percentage of exsheathment. The LEA was used at different concentrations (150, 300, 600, 1200 µg mL-1 of acetonic extract/mL of purified buffer solution (PBS)). The larval exsheathment is concentration, location, phenological stage dependent. All extracts, caused a delay of the percentage of exsheathment over 50% so the AH activity of H. carnosum was confirmed. After addition of PVPP, the % exsheathment was similar to the 150 µg mL-1 concentration. The biplot showed that Loc1(S), Loc4(B), Loc 5(PF), Loc 6(BM) and Loc 6(PF) were isolated from other plant extract sample. Our in vitro study showed that H. carnosum seems to be a promising alternative to AH drugs.

Natural Variation in Physiological Responses of Tunisian Hedysarum carnosum Under Iron Deficiency

“…As an extremophile, H. carnosum also grows on Tunisian saline sodic soils, represented especially by Chotts and Sebkhas ( Dallali et al, 2012 ). This species plays important roles in animal feed due to its high protein contents and tannins ( Aissa et al, 2016 ). H. carnosum is a non-model crop and molecular investigation is difficult because of the lack of molecular data and gene sequences.…”