<i>In vitro</i> assessment of a collagen sponge for engineering urothelial grafts

Sabbagh,; Masters,; Duffy,; Herbage,; Brown,

doi:10.1046/j.1464-410x.1998.00828.x

Cited by 23 publications

(10 citation statements)

References 30 publications

(33 reference statements)

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“…The epithelium on vein graft was confirmed by IHC using pancytokeratin antibody. Pancytokeratin has been found to be a useful marker for the identification of urothelium [29] .…”

Section: Discussionmentioning

confidence: 99%

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Xu¹,

Shen

Liu

et al. 2017

Urol Int

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Objective: To investigate the efficacy of using everted saphenous vein graft for urethral reconstruction. Materials and Methods: Thirty-five adult male rabbits were divided into 7 groups randomly: experimental group A, B, C, D, E, stricture control group and normal control group (n = 5). In experimental groups and the stricture control group, a urethral mucosa defect (1.5 × 0.8 cm) was created in each rabbit. In experimental groups, a 2-cm long saphenous vein graft was harvested and incised longitudinally and urethral reconstruction was carried out using the everted saphenous vein patch. Rabbits in experimental group A-E were killed respectively at 1 week, 2 weeks, 1 month, 3 months, and 1 year postoperatively, and the specimens were obtained for histo-pathological examination. Retrograde urethrography was performed to evaluate urethral patency before sacrifice in group D and the stricture control group. Results: In the histo-pathological study, the vein grafts were visible within first week. The vein graft was completely covered by epithelium 1 month postoperatively. Retrograde urethrograms showed the urethral caliber of experimental rabbits were similar to those of normal. While the stricture control group showed a narrow urethral lumen and urothelium defect. Conclusions: For urethral reconstruction, everted saphenous vein graft can be an ideal substitute material because of its longer survival time and rapid epithelization capacity.

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“…The epithelium on vein graft was confirmed by IHC using pancytokeratin antibody. Pancytokeratin has been found to be a useful marker for the identification of urothelium [29] .…”

Section: Discussionmentioning

confidence: 99%

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Xu¹,

Shen

Liu

et al. 2017

Urol Int

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“…Fibroblasts grow for 7 weeks on dermal collagen preparations from pigs, forming confluent layers with migration of cells into the matrix [12] . Sabbagh et al [13] seeded urothelial cells on collagen films and observed multiple cell layers with a maximal cell proliferation between 5 and 10 days. Further supporting factors of cell growth on collagen scaffolds are growth factor such as epidermal growth factor [14] .…”

Section: Discussionmentioning

confidence: 99%

Different Types of Scaffolds for Reconstruction of the Urinary Tract by Tissue Engineering

et al. 2007

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Introduction: Tissue engineering is an important and expanding field in reconstructive surgery. The ideal biomaterial for urologic tissue engineering should be biodegradable and support autologous cell growth. We examined different scaffolds to select the ideal material for the reconstruction of the bladder wall by tissue engineering. Materials and Methods: We seeded mouse fibroblasts and human keratinocytes in a co-culture model on 13 different scaffolds. The cell-seeded scaffolds were fixed and processed for electron microscopy, hematoxylin and eosin stain, and immunohistochemistry. Cell density and epithelial cell layers were evaluated utilizing a computer-assisted optical measurement system. Results: Depending on the growth pattern, scaffolds were classified into the following three distinct scaffold types: carrier-type scaffolds with very small pore sizes and no ingrowth of the cells. This scaffold type induces a well-differentiated epithelium. Fleece-type scaffolds with fibers and huge pores. We found cellular growth inside the scaffold but no epithelium on top of it. Sponge-type scaffolds with pores between 20 and 40 µm. Cellular growth was observed inside the scaffold and well-differentiated epithelium on top of it. Conclusion: To our knowledge, this is the first time three distinct scaffold types have been reported. All types supported the cell growth. The structure of the scaffolds affects the pattern of cell growth.

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“…Collagen-GAG sponges were used in the present study because variants of these sponges are now widely adapted as clinically useful forms of dermal substitute (produced commercially as Integra TM ) or as tissue engineering cell supports. [40][41][42] These are relatively dense, stable, and mechanically stiff, porous matrices, containing small amounts of the GAG chondroitin sulfate. 43,44 They have previously been shown to support fibroblast force generation in three-dimensional culture, 15,45 and are claimed to reduce contraction clinically, making them good candidate cell substrates to study in this model.…”

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confidence: 99%

Evidence for sequential utilization of fibronectin, vitronectin, and collagen during fibroblast‐mediated collagen contraction

Sethi

Yannas

Mudera

et al. 2002

Wound Repair Regeneration

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Contraction plays a major role in wound healing and is inevitably mediated through the mechanical interaction of fibroblast cytoskeleton and integrins with their extracellular matrix ligands. Cell-matrix attachment is critical for such events. In human dermal fibroblasts most such interactions are mediated by the beta1-type integrins. This study investigated the role played by key components in this system, notably fibronectin, vitronectin, and integrin subcomponents alpha2 and alpha5, which recognize collagen and fibronectin. Inhibition of adhesion through these ligands was studied either by antibody blocking or with fibronectin and/or vitronectin depletion. Functional effects of inhibition were monitored as force generation in collagen-glycosaminoglycan (IntegraTM) sponges, over 20 hours using a culture force monitor. Dose and time-course inhibition studies indicated that initial attachment and force generation (approx. 0-5 hours postseeding) was through fibronectin receptors and this was followed by vitronectin ligand and receptor utilization (4 hours onward). Utilization of the collagen integrin subcomponent alpha2 appeared to be increasingly important between 6 and 16 hours and dominant thereafter. Additionally, there was evidence for functional interdependence between the three ligand systems fibronectin, vitronectin, and collagen. We propose that there is a short cascade of sequential integrin-ligand interactions as cells attach to, extend through, and eventually contract their matrix. (WOUND REP REG 2002;10:-408)

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In vitro assessment of a collagen sponge for engineering urothelial grafts

Cited by 23 publications

References 30 publications

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Different Types of Scaffolds for Reconstruction of the Urinary Tract by Tissue Engineering

Evidence for sequential utilization of fibronectin, vitronectin, and collagen during fibroblast‐mediated collagen contraction

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