<i>In Vitro</i>Application of Langmuir Monolayer Model to Study<i>In Vivo</i>Biological Systems

Tsanova, Asya; Georgiev, Georgi; Lalchev, Zdravko

doi:10.5504/50yrtimb.2011.0034

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…The multilayered structure of meibum facilitates the quick rearrangement of molecules during blinking allowing the tear film lipid layer to withstand the high pressures of blinking. The non-linear dependence of surface pressure on reciprocal compressibility modulus is indicative of the existence of a multicomponent viscoelastic film of Meibomian lipids [ 41 ]. The fact that meibum is viscoelastic and its molecular structure governs its viscoelasticity has been substantiated by rheological studies [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…The values of reciprocal compressibility modulus of meibum samples observed in our study are representative of physiological values because we used a physiologically relevant temperature, 35 °C, pH, 7.4, and salt composition (artificial tears). The variations in other studies are likely to be due to different experimental conditions used such as a different meibum source (bovine), lower temperatures, 25 °C or 28 °C, and a non-physiological subphase of saline [ 36 , 41 ] or tris-buffered saline [ 50 ]. In particular, temperature is a major factor that influences the behavior of meibum [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

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Insights into Tear Film Stability from Babies and Young Adults: A Study of Human Meibum Lipid Conformation and Rheology

Mudgil

Borchman

Ramasubramanian

2018

IJMS

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Babies have the most stable tears and people with dry eye have the least stable tears. Meibum may contribute to tear film stability, so in this study, the hydrocarbon chain conformation and rheology of meibum from babies was studied for the first time. Infrared spectroscopy was used to measure lipid phase transitions. Rheology was measured using Langmuir film technology. Meibum from 25 donors 1 to 13 years old was compared with meibum from 18 donors 13 to 25 years old. The phase transition temperature and lipid order (stiffness) increased with increasing age from 1 to 25 years. The increase in meibum lipid order from 1 to 25 years of age may contribute to the instability of the tear film with age and contribute to films with a higher reciprocal compressibility modulus that are not as compressible and not as viscoelastic. Changes in the lipid phase transition parameters of meibum lipid with dry eye are an exacerbation of the changes observed with age. The lower reciprocal compressibility moduli of meibum films from children and babies compared with meibum from adults reiterates higher stability in their films which spread better, resist deformation, and facilitates their ability to be quickly restored after blinking.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Insights into Tear Film Stability from Babies and Young Adults: A Study of Human Meibum Lipid Conformation and Rheology

Mudgil

Borchman

Ramasubramanian

2018

IJMS

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Lipid Membrane Models for Biomembrane Properties’ Investigation

Sebaaly¹,

Greige‐Gerges²,

Charcosset³

2019

Current Trends and Future Developments on (Bio-) Membranes

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Applications of Brewster angle microscopy from biological materials to biological systems

Daear¹,

Mahadeo²,

Prenner³

2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Brewster angle microscopy (BAM) is a powerful technique that allows for real-time visualization of Langmuir monolayers. The lateral organization of these films can be investigated, including phase separation and the formation of domains, which may be of different sizes and shapes depending on the properties of the monolayer. Different molecules or small changes within a molecule such as the molecule's length or presence of a double bond can alter the monolayer's lateral organization that is usually undetected using surface pressure-area isotherms. The effect of such changes can be clearly observed using BAM in real-time, under full hydration, which is an experimental advantage in many cases. While previous BAM reviews focused more on selected compounds or compared the impact of structural variations on the lateral domain formation, this review provided a broader overview of BAM application using biological materials and systems including the visualization of amphiphilic molecules, proteins, drugs, extracts, DNA, and nanoparticles at the air-water interface.

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In VitroApplication of Langmuir Monolayer Model to StudyIn VivoBiological Systems

Cited by 4 publications

References 21 publications

Insights into Tear Film Stability from Babies and Young Adults: A Study of Human Meibum Lipid Conformation and Rheology

Insights into Tear Film Stability from Babies and Young Adults: A Study of Human Meibum Lipid Conformation and Rheology

Lipid Membrane Models for Biomembrane Properties’ Investigation

Applications of Brewster angle microscopy from biological materials to biological systems

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