<i>In vitro</i> Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of <i>Helichrysum italicum</i> (Roth) G. Don Growing in Central Dalmatia (Croatia)

Staver, Mladenka Malenica; Gobin, Ivana; Ratkaj, Ivana; Petrović, Marinko; Vulinovic, Andrea; Dinarina-Sablic, Marija; Broznić, Dalibor

doi:10.1080/0972060x.2018.1433071

Cited by 33 publications

(36 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The common problem of water system contamination highlights the need to develop simple and effective strategies to counteract both NTMs and FLA, which motivated us to perform this study using the EOs isolated from juniper and immortelle plants, both known for their strong antimicrobial properties (18,27). The use of nontoxic bioactive compounds represents a promising approach to reducing the NTM and FLA burden, considering that most of them do not cause detrimental health effects at effective concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…", Zagreb, Croatia. The same batch had already been used in our earlier studies, which show their chemical composition (11,18,19).…”

Section: Essential Oilsmentioning

confidence: 99%

“…Substances with biological activity in juniper (Juniperus communis L.) EO are α-and β-pinene, β-myrcene, sabinene, limonene, terpinen-4-ol, and β-caryophyllene (11)(12)(13)(14), while immortelle (Helichrysum italicum Roth G. Don fil.) EO contains α-pinene, neryl acetate, β-curcumene, γ-curcumene, β-caryophyllene, limonene, and geranyl acetate (15)(16)(17)(18). Antimicrobial potencies of EOs have widely been investigated so far, but not so many studies focused on proving their efficiency in the development of strategies for the maintenance of water supply systems.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii

Peruč

Tićac

Broznić

et al. 2020

Archives of Industrial Hygiene and Toxicology

Self Cite

View full text Add to dashboard Cite

Acanthamoeba is an opportunistic protozoon, widespread in the aquatic environment, where it can be in endosymbiosis with over 30 pathogenic bacteria, including nontuberculous mycobacteria (NTM). Protozoa play a crucial role in mycobacterial pathogenesis and serve as a reservoir of infection. Since the first step in bacteria making contact with amoebae is adhesion, we were interested in investigating whether essential oils (EOs) can affect it. To that end we investigated the effects of juniper (Juniperus communis) and immortelle (Helichrysum italicum) EOs against Mycobacterium avium, M. intracellulare, and M. gordonae in tap water and against their adhesion to Acanthamoeba castellanii by combining them in synergistic EO concentrations. M. avium and M. intracellulare adhered to A. castellanii to a greater extent than M. gordonae. The adhesion of all NTMs was prevented by the subinhibitory concentrations of EOs. When comparing the effect of synergistic combinations of EOs and the effect of a single concentration from a combination, a higher percentage of adhesion inhibition in all synergistic combinations observed, except against M. gordonae. Neither oil was cytotoxic to A. castellanii. Our findings suggest that the EOs or their components weaken the contact of environmental NTMs and free-living amoebae and indirectly diminish their pathogenic potential, which could be of value in developing strategies for maintenance of water supply systems.

show abstract

Section: Discussionmentioning

confidence: 99%

“…", Zagreb, Croatia. The same batch had already been used in our earlier studies, which show their chemical composition (11,18,19).…”

Section: Essential Oilsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii

Peruč

Tićac

Broznić

et al. 2020

Archives of Industrial Hygiene and Toxicology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sastav EU-a može znatno varirati a time i njegov antimikrobni učinak. EU H. italicum s područja Hrvatske prema dosad dostupnim istraživanjima uobičajeno ima visok sadržaj α-pinena, γ-i α-kurkumena, α-i β-selinena i neril acetata 17,19,33 . Kako je α-pinen bio dominantna komponenta i EU-a plodova borovice (Juniperus communis) čiji smo učinak prema NTM-u testirali u prethodnom istraživanju, smatrali smo da je upravo ta komponenta odgovorna za antimikobakterijski učinak 36 .…”

Section: Uvodunclassified

“…volumen osnovne suspenzije koji treba razrijediti da bismo dobili izvornu (stock) koncentraciju od 200 mg/ml. Malenica Staver i suradnici odredili su kemijski sastav ovog EU-a smilja metodom plinske kromatografije i spektrometrije masa u kojem najveći udio čine α-pinen (21,6 %) i γ-kurkumen (21,6 %), slijedi β-selinen (6,5 %), isoitalicen (5,4 %), β-kariofilen (4,9 %) i α-kurkumen (4,5 %)19 .…”

unclassified

Influence of essential oil Helichrysum italicum (Roth) G. Don on the formation of non-tuberculous mycobacterial biofilm

et al. 2018

Self Cite

View full text Add to dashboard Cite

Sažetak. Cilj: Ispitati antimikrobni i antioksidacijski učinak te sposobnost inhibicije biofilma eteričnog ulja (EU) smilja [Helichrysum italicum (Roth) G. Don (H. italicum)] prema netuberkuloznim mikobakterijama M. avium i M. intracellulare. Materijali i metode: Za određivanje minimalne inhibicijske koncentracije (MIK) i minimalne baktericidne koncentracije (MBK) EU H. italicum prema M. avium i M. intracellulare korištena je metoda mikrodilucije u bujonu. Preživljavanje mikobakterija u Middlebrook 7H9 bujonu pod utjecajem EU-a smilja praćeno je subkultivacijom suspenzija nulti, 1., 4. i 8. dan. Za određivanje inhibicije stvaranja biofilma u vodi primijenjena je metoda bojenja biofilma s kristal violetom. Antioksidacijski učinak MIK koncentracije EU-a određen je DPPH (2,2-difenil-1-pikril-hidrazil) metodom. Rezultati: MIK i MBK vrijednost EU-a smilja iznosila je 3,2 mg/ml. Koncentracija ½ MIK vrijednosti dovela je nakon prvog dana do redukcije vijabilnosti M. avium za približno 2 log 10 odnosno 2,5 log 10 za M. intracellulare, dok je četvrti dan zabilježena potpuna inhibicija porasta obiju vrsti mikobakterija. M. avium pokazao je veću tendenciju stvaranja biofilma. Koncentracija od ¼ i ½ MIK vrijednosti dovela je do statistički značajne inhibicije stvaranja biofilma ispitivanih mikobakterija. MIK koncentracija EU H. italicum je nakon 60 minuta inhibirala 38,2 % DPPH radikala. Zaključak: Subinhibitorne koncentracije EU-a smilja značajno reduciraju vijabilnost M. avium i M. intracellulare i dovode do značajne inhibicije stvaranja biofilma ispitivanih mikobakterija u vodi, što bi omogućilo primjenu niskih efektivnih koncentracija ovog eteričnog ulja, koje nemaju citotoksični učinak, kao prirodnog dezinficijensa u vodi. Abstract. Aim: To investigate the antimicrobial and antioxidative effect of the essential oil (EO) of immortelle [Helichrysum italicum (Roth) G. Don (H. italicum)] and its ability to inhibit biofilm of nontuberculous mycobacteria, M. avium and M. intracellulare. Materials and methods: a broth microdilution method was used to determine the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of the H. italicum EO. Survival of mycobacteria in Middlebrook 7H9 broth under the influence of H. italicum EO was monitored by subcultivation of suspension of 0, 1, 4 and 8 days. For determination of the inhibition of biofilm formation in water, the biofilm coloring method with crystal violet was applied. The antioxidant effect of MIC concentrations of H. italicum EO was determined by DPPH (2,2-diphenyl-1-picrylhydrazil) method. Results: MIC and MBC value of immortelle EO was 3.2 mg/ml. Treatment of mycobacteria with H. italicum EO at one half times the MIC resulted in approximately 2 log 10 reduction of M. avium and 2.5 log 10 M. intracellulare respectively over 24 h, and complete reduction of both mycobacteria on the fourth day. M. avium showed a greater tendency to form biofilms. The concentration of one-quarter and one-half of the MIC values resulted in statistically significan...

show abstract

Chemical Characterization of Rare Unifloral Honeys of Ailanthus (Ailanthus altissima), Fennel (Foenicum vulgare), and Raspberry (Rubus idaeus) and their Antimicrobial and Antioxidant Activity

Saftić Martinović,

Birkic,

Pavlešić

et al. 2024

Agric Res

View full text Add to dashboard Cite

In vitro Antiproliferative and Antimicrobial Activity of the Essential Oil from the Flowers and Leaves of Helichrysum italicum (Roth) G. Don Growing in Central Dalmatia (Croatia)

Cited by 33 publications

References 34 publications

Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii

Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii

Influence of essential oil Helichrysum italicum (Roth) G. Don on the formation of non-tuberculous mycobacterial biofilm

Chemical Characterization of Rare Unifloral Honeys of Ailanthus (Ailanthus altissima), Fennel (Foenicum vulgare), and Raspberry (Rubus idaeus) and their Antimicrobial and Antioxidant Activity

Contact Info

Product

Resources

About