In vitroantioxidant and anti-cholinesterase activities ofRhizophora mucronata

Abstract

Context: Rhizophora mucronata Lam. (Rhizophoraceae), commonly known as Asiatic mangrove, has been used traditionally among Asian countries as folk medicine. Objective: This study investigates the cholinesterase inhibitory potential and antioxidant activities of R. mucronata. Materials and method: Rhizophora mucronata leaves were successively extracted using solvents of varying polarity and a dosage of 100-500 mg/ml were used for each assay. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities were assessed according to the method of Ellman. In vitro antioxidant activity was assessed using free radical scavenging, reducing power, and metal-chelating activity (duration -3 months). Total phenolic and flavonoid content were quantified spectrophotometrically. Compound characterization was done using column chromatography, NMR, FTIR, and LC-MS analysis. Results: Methanolic leaf extract (500 mg/ml) exhibited the highest inhibitory activity against AChE (92.73 ± 0.54%) and BuChE (98.98 ± 0.17%), with an IC 50 value of 59.31 ± 0.35 and 51.72 ± 0.33 mg/ml, respectively. Among the different solvent extracts, methanolic extract exhibited the highest antioxidant activity with an IC 50 value of 47.39 ± 0.43, 401.45 ± 18.52, 80.23 ± 0.70, and 316.47 ± 3.56 mg/ml for DPPH, hydroxyl, nitric oxide radical, and hydrogen peroxide, respectively. Total polyphenolic and flavonoid contents in methanolic extract were observed to be 598.13 ± 1.85 mg of gallic acid equivalent and 48.85 ± 0.70 mg of rutin equivalent/ mg of extract. Compound characterization illustrated (+)-catechin as the bioactive compound responsible for cholinesterase inhibitory and antioxidant activities. Conclusion: The presence of rich source of flavonoids, in particular catechin, might be responsible for its cholinesterase inhibitory and antioxidant activities.

Phylogenetic diversity and antioxidant activities of culturable fungal endophytes associated with the mangrove species Rhizophora stylosa and R. mucronata in the South China Sea

“…R . mucronata has been shown to have highly effective scavenging towards DPPH, hydroxyl, nitric oxide radicals, and hydrogen peroxide radicals [ 44 , 45 ]. To efficiently utilize the mangrove fungal sources available from a single fungal strain, we attempted to explore the antioxidants of isolated fungi by changing the cultivation media.…”

Diversity and Characterization of Endophytic Fungi Isolated From the Tropical Mangrove Species, Rhizophora mucronata, and Identification of Potential Antagonists Against the Soil-Borne Fungus, Fusarium solani

“…Different parts of R . mucronata such as the bark and leaves have been used traditionally as astringent and antiseptic, and have been shown to possess activities against bacteria, ulcers, and inflammation (Kokpol et al, 1990 ; Suganthy and Pandima Devi, 2016 ). Furthermore, pharmacological studies have shown that the leaf exhibits antiviral, anti-inflammatory, and anti-diabetic activities (Gaffar et al, 2011 ).…”

Estado Da Arte Nos Estudos Relacionados À Problemática Dos Terremotos

“…A figura 1 representa estudos destacando as atividades biológicas de cada gênero; Avicennia, Conocarpus, Laguncularia e Rhizophora, encontrados no manguezal brasileiro. ABDEL-HAMEED; BAZAID, 2013) (9) ,(ODA, 2017) (10) , (RAZA et al, 2016) (11) ; Atividades biológicas Rhizophora (KUSUMA; KUMAR; BOOPALAN, 2011) (12) , (MELCHOR et al, 2001;FERREIRA et al, 2011;MUSTHAFA et al, 2013) (13) , (SUGANTHY;PANDIMA DEVI, 2016;BERENGUER et al, 2006) (14) , (RAMALINGAM; RAJARAM, 2018) (15) , (CHAKRABORTY; RAOLA, 2017) (16) .…”