<i>In‐vitro</i>antimicrobial susceptibility of Australian isolates of<i>Treponema hyodysenteria</i>

Sc, Smith; Muir, T; Holmes, Matthew; Pj, Coloe

doi:10.1111/j.1751-0813.1991.tb03115.x

Cited by 11 publications

(12 citation statements)

References 6 publications

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“…When compared with the MIC 90 of S. hyodysenteriae strains against tiamulin, the North American isolates of S. pilosicoli were similar. 2,15,16,20,21,29,30,38 Thus, as previously shown for S. hyodysenteriae, 8,[15][16][17]20,21,29,30,38 S. pilosicoli is highly sensitive to tiamulin.…”

Section: Discussionsupporting

confidence: 53%

“…However, when compared with S. hyodysenteriae, the MIC 90 of North American S. pilosicoli isolates against lincomycin and gentamicin were similar. 2,[14][15][16]20,21,29,30,38 Diarrheal disease and reduced performance associated with S. pilosicoli have been reported from all major swine producing countries of North America, Europe, and Australia. 1,5,6,9,10,12,19,[24][25][26][27][32][33][34][35][36][37] The prevalence of S. pilosicoli infection in finisher farms of a multisite production system in the United States was estimated recently to be approximately 50%.…”

Section: Discussionmentioning

confidence: 99%

“…2,8,9,[14][15][16][17][20][21][22]29,30,38,39 Because S. pilosicoli was recognized only recently, little information is available about the in vitro antimicrobial susceptibility of this spirochete. 8,9,14,21,29,30 The results of the present in vitro study indicated that 100% of the isolates were susceptible to carbadox and tiamulin, whereas the suceptibility to lincomycin and gentamicin differed among the isolates. None of the isolates were resistant to all 4 antimicrobial agents, but isolates UNL-53 and UNL-54, both from the same sampling on the same farm, were resistant to both lincomycin and gentamicin.…”

Section: Discussionmentioning

confidence: 99%

“…There have been several reports on the in vitro susceptibility of S. hyodysenteriae 2,8,9,14-17,20 -22,29,30,38,39 and some other uncharacterized WBHIS 8,9,14,21,29,30 to selected antimicrobial agents. Because S. pilosicoli is a newly recognized intestinal spirochete, there is a need to determine the in vitro antimicrobial susceptibility of this organism so that disease-specific control strategies can be implemented.…”

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confidence: 99%

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In Vitro Activity of Four Antimicrobial Agents against North American Isolates of Porcine Serpulina Pilosicoli

et al. 1998

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Abstract. Porcine colonic spirochetosis is a nonfatal diarrheal disease that affects pigs during the growing and finishing stages of production. The disease is caused by Serpulina pilosicoli, a newly recognized species of pathogenic intestinal spirochete. Antimicrobial therapy aimed at reducing the infection may be helpful in controlling spirochetal diarrhea. In this study, the in vitro antimicrobial susceptibilities of the reference isolate S. pilosicoli P43/6/78 from the United Kingdom and 19 field isolates obtained from pigs in Canada (n ϭ 5) and the United States (n ϭ 14) were determined against the antimicrobial agents carbadox, gentamicin, lincomycin, and tiamulin, all of which are commonly used for control of the related pathogenic intestinal spirochete S. hyodysenteriae. Additionally, the susceptibility or resistance of each isolate against each antimicrobial agent was estimated on the basis of available data on the in vitro antimicrobial susceptibility breakpoints of S. hyodysenteriae. Each isolate was identified on the basis of phenotypic and genotypic markers, and the minimum inhibitory concentration of each antimicrobial agent was determined by the agar-dilution method. All the isolates were susceptible to carbadox and tiamulin. The percentages of isolates susceptible, intermediate, and resistant to lincomycin were 42.1%, 42.1%, and 15.8%, respectively. Slightly less than half of the isolates (47.4%) were susceptible to gentamicin, and the remainder (52.6%) were resistant. Implementation of rational control measures to reduce infection by S. pilosicoli should improve overall health and productivity in swine herds.Until recently, porcine hemolytic intestinal spirochetes consisted of 2 taxonomically recognized species in the genus Serpulina: the strongly ␤-hemolytic spirochete Serpulina hyodysenteriae, the agent of swine dysentery, and the weakly ␤-hemolytic intestinal spirochete (WBHIS) S. innocens, a nonpathogenic spirochete of the swine colon. 7,13,31 However, an association between a WBHIS, recently classified as S. pilosicoli, and a nonfatal diarrheal disease of growing pigs, called porcine intestinal spirochetosis or porcine colonic spirochetosis (PCS), has been recognized. 1,[5][6][7][8][9][10][11][12]19,[24][25][26][27][28][32][33][34][35][36][37] With more intensive swine production, where the efficiency of weight gain is closely monitored, PCS has been recognized as a major contributing factor to reduced performance in growing and finishing pigs. 5,6,8,9,10,12,34 Koch's postulates for S. pilosicoli have been fulfilled using gnotobiotic pigs 25 and conventional pigs. 3,5,[33][34][35] Conventional pigs challenge exposed with S. pilosicoli have diarrhea and reduced performance as a result of colonization and inflammation of the cecum and colon. 3,5,[33][34][35] Clinical signs of S. pilosicoli infection consist of diarrhea, which is transient to persistent. The feces from diarrheic pigs are watery to mucoid in consistency,

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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In Vitro Activity of Four Antimicrobial Agents against North American Isolates of Porcine Serpulina Pilosicoli

et al. 1998

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“…To determine susceptibility of B. hyodysenteriae to doxycycline, the ADM was used. In most laboratories, quantitative susceptibility testing of brachyspiras is commonly done by diluting the antibacterial substance in agar media (Kitai et al 1987;Ronne and Szancer 1990;Smith et al 1991;Trott et al 1996;D uhamel et al 1998;Hommez et al 1998;Fossi et al 1999) or by the broth dilution method (Hayashi et al 1988;Buller and Hampson 1994;Karlsson et al 2002). Apart from applying the agar dilution method, which has been used by our laboratory on a daily basis for several years, a semi-quantitative method, namely the Epsilometer test for B. hyodysenteriae susceptibility to doxycycline, was analyzed.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Brachyspira hyodysenteriae Isolates to Doxycycline using Agar Dilution and Epsilometer Test

Lobová¹,

Čížek²

2004

Acta Vet. Brno

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Lobová D., A. âíÏek: Susceptibility of Brachyspira hyodysenteriae Isolates to Doxycycline using Agar Dilution and Epsilometer Test. Acta Vet. Brno 2004, 73: 329-333.The agar dilution method and the Epsilometer test were used to evaluate antibacterial susceptibility of 49 Brachyspira hyodysenteriae field isolates to doxycycline. A comparison of results obtained by the two methods revealed agreement for 84% of B. hyodysenteriae isolates; with 16% of isolates the Minimal Inhibitory Concentration values differing by two and more dilutions. Out of the total of B. hyodysenteriae isolates tested, 36 (73%) were classified as sensitive, 13 (27%) as intermediary, and none of the strains were found doxycycline-resistant. The demonstrated susceptibility of B. hyodysenteriae isolates to doxycycline suggests the possibility of using it for combination therapy with pleuromutilins of swine dysentery. The Epsilometer test has proved a practical alternative to dilution methods of B. hyodysenteriae susceptibility testing.

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Comparison of methods for antimicrobial susceptibility testing and MIC values for pleuromutilin drugs for Brachyspira hyodysenteriae isolated in Germany

Rohde¹,

Keßler²,

Baums³

et al. 2004

Veterinary Microbiology

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In‐vitroantimicrobial susceptibility of Australian isolates ofTreponema hyodysenteria

Cited by 11 publications

References 6 publications

In Vitro Activity of Four Antimicrobial Agents against North American Isolates of Porcine Serpulina Pilosicoli

In Vitro Activity of Four Antimicrobial Agents against North American Isolates of Porcine Serpulina Pilosicoli

Susceptibility of Brachyspira hyodysenteriae Isolates to Doxycycline using Agar Dilution and Epsilometer Test

Comparison of methods for antimicrobial susceptibility testing and MIC values for pleuromutilin drugs for Brachyspira hyodysenteriae isolated in Germany

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